BioPAX pathway converted from "DNA replication initiation" in the Reactome database.DNA replication initiationDNA replication initiationThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>2.7.7.6The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer at the originThe primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer at the originThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Converted from EntitySet in ReactomeReactome DB_ID: 305951nucleoplasmGO0005654NTP [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityGTP [nucleoplasm]CTP [nucleoplasm]UTP [nucleoplasm]ATP [nucleoplasm]Reactomehttp://www.reactome.orgChEBI15996ChEBI17677ChEBI15713ChEBI30616Reactome DB_ID: 101887161DNA polymerase alpha:primase:DNA polymerase alpha:origin complex [nucleoplasm]DNA polymerase alpha:primase:DNA polymerase alpha:origin complexReactome DB_ID: 101886961DNA polymerase alpha:primase [nucleoplasm]DNA polymerase alpha:primaseReactome DB_ID: 101886841UniProt:B0S687pola2Danio rerioNCBI Taxonomy7955UniProtB0S687Chain Coordinates1EQUAL598EQUALReactome DB_ID: 101886801UniProt:E7FB68pola1UniProtE7FB681EQUAL1462EQUALReactome DB_ID: 101886941PRIM1:PRIM2 [nucleoplasm]PRIM1:PRIM2Reactome DB_ID: 101886921UniProt:B8JIP2prim2UniProtB8JIP21EQUAL509EQUALReactome DB_ID: 101886881UniProt:F1QKT6prim1UniProtF1QKT61EQUAL420EQUALReactome Database ID Release 7510188694Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188694ReactomeR-DRE-96688121Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-9668812.1Reactome Database ID Release 7510188696Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188696ReactomeR-DRE-685071Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68507.1Reactome DB_ID: 101887141DNA polymerase epsilon:origin complex [nucleoplasm]DNA polymerase epsilon:origin complexReactome DB_ID: 684191origin of replication [nucleoplasm]origin of replicationARSautonomously replicating sequenceoriginReactome DB_ID: 101887121DNA polymerase epsilon [nucleoplasm]DNA polymerase epsilonReactome DB_ID: 101887021Ghost homologue of POLE2 [nucleoplasm]Ghost homologue of POLE2Reactome DB_ID: 101887061UniProt:Q6PBL4pole3UniProtQ6PBL42EQUAL147EQUALReactome DB_ID: 101887101UniProt:F1RCI6pole4UniProtF1RCI62EQUAL117EQUALReactome DB_ID: 101887001UniProt:B0V351poleUniProtB0V3511EQUAL2286EQUALReactome Database ID Release 7510188712Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188712ReactomeR-DRE-684831Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68483.1Reactome Database ID Release 7510188714Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188714ReactomeR-DRE-684851Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68485.1Reactome Database ID Release 7510188716Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188716ReactomeR-DRE-685101Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68510.1Reactome DB_ID: 101887181RNA primer:origin duplex:DNA polymerase alpha:primase complex [nucleoplasm]RNA primer:origin duplex:DNA polymerase alpha:primase complexReactome DB_ID: 684221RNA primer [nucleoplasm]RNA primerReactome DB_ID: 101886961Reactome DB_ID: 684191Reactome Database ID Release 7510188718Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188718ReactomeR-DRE-684231Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68423.1Reactome DB_ID: 101887121PHYSIOL-LEFT-TO-RIGHTACTIVATIONReactome DB_ID: 10188716GO0003899GO molecular functionReactome Database ID Release 7510188719Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188719Reactome Database ID Release 7510188721Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188721ReactomeR-DRE-689131Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68913.1At the beginning of this reaction, 1 molecule of 'DNA polymerase alpha:primase:DNA polymerase alpha:origin complex', and 1 molecule of 'NTP' are present. At the end of this reaction, 1 molecule of 'DNA polymerase epsilon', and 1 molecule of 'RNA primer:origin duplex:DNA polymerase alpha:primase complex' are present.<br><br> This reaction takes place in the 'nucleus' and is mediated by the 'DNA-directed RNA polymerase activity' of 'DNA polymerase alpha:primase'.<br>6693436Pubmed1984DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha.Wang, TSHu, SZKorn, DJ Biol Chem 259:1854-65inferred by electronic annotationIEAGOIEA2.7.7.7The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer at the originThe polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer at the originThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 296041dATP [ChEBI:16284]dATP2'-deoxyadenosine 5'-triphosphateDeoxyadenosine triphosphateDeoxyadenosine 5'-triphosphateChEBI16284Reactome DB_ID: 301851dCTP [ChEBI:16311]dCTP2'-Deoxycytidine 5'-triphosphateDeoxycytidine triphosphatedeoxycytidine 5'-triphosphateChEBI16311Reactome DB_ID: 298921dGTP [ChEBI:16497]dGTPDeoxyguanosine 5'-triphosphate2'-deoxyguanosine 5'-triphosphateDeoxyguanosine triphosphateChEBI16497Reactome DB_ID: 301871dTTP [ChEBI:18077]dTTPDeoxythymidine triphosphateTTPDeoxythymidine 5'-triphosphateChEBI18077Reactome DB_ID: 101887181Reactome DB_ID: 101888741RNA primer-DNA primer:origin duplex [nucleoplasm]RNA primer-DNA primer:origin duplexReactome DB_ID: 101887181Reactome DB_ID: 684241DNA primer [nucleoplasm]DNA primerReactome Database ID Release 7510188874Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188874ReactomeR-DRE-684251Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68425.1PHYSIOL-LEFT-TO-RIGHTACTIVATIONReactome DB_ID: 10188696GO0003887GO molecular functionReactome Database ID Release 7510188875Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188875Reactome Database ID Release 7510188877Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10188877ReactomeR-DRE-689501Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68950.1At the beginning of this reaction, 1 molecule of 'dTTP', 1 molecule of 'dGTP', 1 molecule of 'dATP', 1 molecule of 'RNA primer:origin duplex:DNA polymerase alpha:primase complex', and 1 molecule of 'dCTP' are present. At the end of this reaction, 1 molecule of 'RNA primer-DNA primer:origin duplex' is present.<br><br> This reaction takes place in the 'nucleus' and is mediated by the 'DNA-directed DNA polymerase activity' of 'DNA polymerase alpha:primase'.<br>6094569Pubmed1984Purification of a DNA polymerase-DNA primase complex from calf thymus glandsChang, L MRafter, EAugl, CBollum, F JJ. Biol. Chem. 259:14679-87inferred by electronic annotationIEAGOIEAReactome Database ID Release 7510302827Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10302827ReactomeR-DRE-689521Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-68952.1GO0006270GO biological processDNA polymerases are not capable of de novo DNA synthesis and require synthesis of a primer, usually by a DNA-dependent RNA polymerase (primase) to begin DNA synthesis. In eukaryotic cells, the primer is synthesized by DNA polymerase alpha:primase. First, the DNA primase portion of this complex synthesizes approximately 6-10 nucleotides of RNA primer and then the DNA polymerase portion synthesizes an additional 20 nucleotides of DNA (Frick & Richardson 2002; Wang et al 1984).11395402Pubmed2002DNA primases.Frick, DNRichardson, CCAnnu Rev Biochem 70:39-80inferred by electronic annotationIEAGOIEA