BioPAX pathway converted from "p53-Independent G1/S DNA damage checkpoint" in the Reactome database. p53-Independent G1/S DNA damage checkpoint p53-Independent G1/S DNA damage checkpoint This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> p53-Independent DNA Damage Response p53-Independent DNA Damage Response This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> Ubiquitin Mediated Degradation of Phosphorylated Cdc25A Ubiquitin Mediated Degradation of Phosphorylated Cdc25A This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> 2.7.11.1 Phosphorylation of Cdc25A at Ser-123 by Chk2 Phosphorylation of Cdc25A at Ser-123 by Chk2 This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> Reactome DB_ID: 10249915 1 nucleoplasm GO 0005654 UniProt:A0A287B2P5 CDC25A Reactome http://www.reactome.org Sus scrofa NCBI Taxonomy 9823 UniProt A0A287B2P5 Chain Coordinates 1 EQUAL 524 EQUAL Reactome DB_ID: 29358 1 ATP(4-) [ChEBI:30616] ATP(4-) Adenosine 5'-triphosphate atp ATP ChEBI 30616 Reactome DB_ID: 113582 1 ADP(3-) [ChEBI:456216] ADP(3-) ADP trianion 5&apos;-O-[(phosphonatooxy)phosphinato]adenosine ADP ChEBI 456216 Reactome DB_ID: 10250068 1 O-phospho-L-serine at 123 (in Homo sapiens) 123 EQUAL O-phospho-L-serine [MOD:00046] 1 EQUAL 524 EQUAL PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 10250085 UniProt:A0A287A7U1 CHEK2 UniProt A0A287A7U1 O-phospho-L-threonine at 68 (in Homo sapiens) 68 EQUAL O-phospho-L-threonine [MOD:00047] O-phospho-L-serine at 379 (in Homo sapiens) 379 EQUAL O-phospho-L-threonine at 383 (in Homo sapiens) 383 EQUAL O-phospho-L-threonine at 387 (in Homo sapiens) 387 EQUAL 1 EQUAL 543 EQUAL GO 0004674 GO molecular function Reactome Database ID Release 82 10250086 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10250086 Reactome Database ID Release 82 10250088 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10250088 Reactome R-SSC-69608 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-69608.1 Detection of DNA damage caused by ionizing radiation results in the phosphorylation of Cdc25A at Ser-123 by Chk2 (Mailand et al. 2001). 11298456 Pubmed 2001 The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis. Mailand, N SyljuĆ„sen, RG Lukas, J Nature 410:842-7 inferred by electronic annotation IEA GO IEA Proteolytic degradation of ubiquitinated-Cdc25A Proteolytic degradation of ubiquitinated-Cdc25A This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> Reactome DB_ID: 10250064 1 cytosol GO 0005829 Ub-p-S123-CDC25A [cytosol] Ub-p-S123-CDC25A Converted from EntitySet in Reactome Reactome DB_ID: 10249581 1 Ub [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity RPS27A [cytosol] UBC [cytosol] UBA52 [cytosol] UniProt A0A287AZA7 UniProt P0CG68 UniProt P63053 Reactome DB_ID: 10250062 1 O-phospho-L-serine at 123 (in Homo sapiens) 123 EQUAL 1 EQUAL 524 EQUAL Reactome Database ID Release 82 10250064 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10250064 Reactome R-SSC-69589 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-69589.1 Converted from EntitySet in Reactome Reactome DB_ID: 10249581 1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 10249683 26S proteasome [cytosol] 26S proteasome Reactome DB_ID: 10249613 1 UniProt:A1XQU1 PSMB7 UniProt A1XQU1 44 EQUAL 277 EQUAL Reactome DB_ID: 10249619 1 UniProt:F2Z5J1 PSMC1 UniProt F2Z5J1 2 EQUAL 440 EQUAL Reactome DB_ID: 10249639 1 UniProt:F1RKW8 PSMD11 UniProt F1RKW8 2 EQUAL 422 EQUAL Reactome DB_ID: 10249663 1 UniProt:F1SGM3 PSME2 UniProt F1SGM3 2 EQUAL 239 EQUAL Reactome DB_ID: 10249603 1 UniProt:A0A5G2R6J1 PSMB2 UniProt A0A5G2R6J1 1 EQUAL 201 EQUAL Reactome DB_ID: 10249591 1 UniProt:A0A5G2QL81 PSMA4 UniProt A0A5G2QL81 1 EQUAL 261 EQUAL Reactome DB_ID: 10249601 1 UniProt:A0A287BRV0 PSMB10 UniProt A0A287BRV0 40 EQUAL 273 EQUAL Reactome DB_ID: 10249633 1 UniProt:A0A287BG14 PSMC6 UniProt A0A287BG14 1 EQUAL 389 EQUAL Reactome DB_ID: 10249661 1 UniProt:Q64L94 PSME1 UniProt Q64L94 1 EQUAL 249 EQUAL Reactome DB_ID: 10249615 1 Ghost homologue of PSMB8 [cytosol] Ghost homologue of PSMB8 Reactome DB_ID: 10249679 1 UniProt:F1S9C7 PSMB11 UniProt F1S9C7 50 EQUAL 300 EQUAL Converted from EntitySet in Reactome Reactome DB_ID: 10249631 1 Homologues of PSMC5 [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity PSMC5 [cytosol] PSMC5 [cytosol] UniProt P62197 UniProt A0A5G2QJH1 Reactome DB_ID: 10249587 1 UniProt:A0A5G2QZH5 PSMA2 UniProt A0A5G2QZH5 2 EQUAL 234 EQUAL Reactome DB_ID: 10249599 1 UniProt:A0A287BPP9 PSMB1 UniProt A0A287BPP9 29 EQUAL 241 EQUAL Reactome DB_ID: 10249649 1 UniProt:A0A288CG47 PSMD4 UniProt A0A288CG47 1 EQUAL 377 EQUAL Reactome DB_ID: 10249605 1 UniProt:A0A286ZN52 PSMB3 UniProt A0A286ZN52 2 EQUAL 205 EQUAL Reactome DB_ID: 10249611 1 UniProt:A0A5G2R4Y9 PSMB6 UniProt A0A5G2R4Y9 35 EQUAL 239 EQUAL Reactome DB_ID: 10249645 1 Ghost homologue of PSMD2 [cytosol] Ghost homologue of PSMD2 Reactome DB_ID: 10249675 1 UniProt:F1SBA5 PSMA8 UniProt F1SBA5 1 EQUAL 256 EQUAL Reactome DB_ID: 10249671 1 UniProt:A0A287APQ3 PSME4 UniProt A0A287APQ3 1 EQUAL 1843 EQUAL Reactome DB_ID: 10249641 1 UniProt:A0A480SMR6 PSMD12 UniProt A0A480SMR6 2 EQUAL 456 EQUAL Reactome DB_ID: 10249585 1 UniProt:A0A5G2QI19 PSMA1 UniProt A0A5G2QI19 1 EQUAL 263 EQUAL Reactome DB_ID: 10249597 1 UniProt:A0A287BRR7 PSMA7 UniProt A0A287BRR7 1 EQUAL 248 EQUAL Reactome DB_ID: 10249617 1 UniProt:Q2PYM7 PSMB9 UniProt Q2PYM7 21 EQUAL 219 EQUAL Reactome DB_ID: 10249621 1 UniProt:F1SB53 PSMC2 UniProt F1SB53 2 EQUAL 433 EQUAL Reactome DB_ID: 10249659 1 UniProt:A0A286ZLP7 PSMD9 UniProt A0A286ZLP7 1 EQUAL 223 EQUAL Reactome DB_ID: 10249653 1 UniProt:F1SGF1 PSMD6 UniProt F1SGF1 1 EQUAL 389 EQUAL Reactome DB_ID: 10249595 1 Ghost homologue of PSMA6 [cytosol] Ghost homologue of PSMA6 Reactome DB_ID: 10249665 1 UniProt:P61291 PSME3 UniProt P61291 2 EQUAL 254 EQUAL Reactome DB_ID: 10249607 1 Ghost homologue of PSMB4 [cytosol] Ghost homologue of PSMB4 Reactome DB_ID: 10249609 1 UniProt:A0A286ZS34 UniProt A0A286ZS34 60 EQUAL 263 EQUAL Reactome DB_ID: 10249657 1 UniProt:A0A287B5Q7 PSMD8 UniProt A0A287B5Q7 1 EQUAL 350 EQUAL Reactome DB_ID: 10249593 1 UniProt:F2Z5K2 PSMA5 UniProt F2Z5K2 1 EQUAL 241 EQUAL Reactome DB_ID: 10249667 1 UniProt:F1S870 PSMF1 UniProt F1S870 1 EQUAL 271 EQUAL Reactome DB_ID: 10249623 1 UniProt:A0A5G2QSU8 PSMC3 UniProt A0A5G2QSU8 1 EQUAL 439 EQUAL Reactome DB_ID: 10249681 1 Ghost homologue of SEM1 [cytosol] Ghost homologue of SEM1 Reactome DB_ID: 10249589 1 UniProt:A0A5G2QWE9 PSMA3 UniProt A0A5G2QWE9 2 EQUAL 255 EQUAL Reactome DB_ID: 10249637 1 UniProt:A0A287BTN9 PSMD10 UniProt A0A287BTN9 1 EQUAL 226 EQUAL Reactome DB_ID: 10249651 1 UniProt:A0A480QIP5 PSMD5 UniProt A0A480QIP5 2 EQUAL 504 EQUAL Reactome DB_ID: 10249625 1 UniProt:A0A287B4P8 PSMC4 UniProt A0A287B4P8 1 EQUAL 418 EQUAL Reactome DB_ID: 10249643 1 UniProt:A0A5G2R6X9 PSMD13 UniProt A0A5G2R6X9 1 EQUAL 376 EQUAL Reactome DB_ID: 10249583 1 UniProt:A0A480DPN6 PSMD14 UniProt A0A480DPN6 1 EQUAL 310 EQUAL Reactome DB_ID: 10249647 1 UniProt:F1RXA7 PSMD3 UniProt F1RXA7 1 EQUAL 534 EQUAL Reactome DB_ID: 10249655 1 UniProt:A0A287AEH0 PSMD7 UniProt A0A287AEH0 1 EQUAL 324 EQUAL Reactome DB_ID: 10249635 1 UniProt:F1SMW9 PSMD1 UniProt F1SMW9 1 EQUAL 953 EQUAL Reactome Database ID Release 82 10249683 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10249683 Reactome R-SSC-68819 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-68819.1 GO 0004175 GO molecular function Reactome Database ID Release 82 10249684 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10249684 Reactome Database ID Release 82 10250066 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10250066 Reactome R-SSC-69600 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-69600.1 At the beginning of this reaction, 1 molecule of 'Ubiquitinated Phospho-Cdc25A' is present. At the end of this reaction, 1 molecule of 'Amino Acid' is present.<br><br> This reaction takes place in the 'cytosol' and is mediated by the 'endopeptidase activity' of '26S proteasome' (Bernardi et al. 2000).<br> 10828887 Pubmed 2000 Cdc25A stability is controlled by the ubiquitin-proteasome pathway during cell cycle progression and terminal differentiation Bernardi, R Liebermann, DA Hoffman, B Oncogene 19:2447-54 inferred by electronic annotation IEA GO IEA 2.7.11.1 Phosphorylation of Cdc25A at Ser-123 by Chk1 Phosphorylation of Cdc25A at Ser-123 by Chk1 This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> Reactome DB_ID: 10249915 1 1 EQUAL 524 EQUAL Reactome DB_ID: 29358 1 Reactome DB_ID: 113582 1 Reactome DB_ID: 10250068 1 O-phospho-L-serine at 123 (in Homo sapiens) 123 EQUAL 1 EQUAL 524 EQUAL PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 10250074 UniProt:F1S774 UniProt F1S774 O-phospho-L-serine at 317 (in Homo sapiens) 317 EQUAL O-phospho-L-serine at 345 (in Homo sapiens) 345 EQUAL 1 EQUAL 476 EQUAL Reactome Database ID Release 82 10250075 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10250075 Reactome Database ID Release 82 10250077 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10250077 Reactome R-SSC-69604 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-69604.1 Detection of DNA damage caused by ionizing radiation results in the phosphorylation of Cdc25A at Ser-123 by Chk1, inhibiting Cdc25A. 12399544 Pubmed 2002 Disruption of the checkpoint kinase 1/cell division cycle 25A pathway abrogates ionizing radiation-induced S and G2 checkpoints. Zhao, H Watkins, JL Piwnica-Worms, H Proc Natl Acad Sci U S A 99:14795-800 inferred by electronic annotation IEA GO IEA Reactome Database ID Release 82 10349438 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10349438 Reactome R-SSC-69601 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-69601.1 cdc25A protein is degraded by the ubiquitin-proteasome machinery in both terminally differentiating and cycling cells (Bernardi et al. 2000). inferred by electronic annotation IEA GO IEA Reactome Database ID Release 82 10349440 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10349440 Reactome R-SSC-69610 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-69610.1 In response to DNA damage due to exposure to ultraviolet light or to ionizing radiation, Cdc25A is phosphorylated by Chk1 or Chk2. The phosphorylation of Cdc25A at ser-123, in response to DNA damage from ionizing radiation is a signal for ubiquitination and subsequent degradation of Cdc25A. The destruction of Cdc25A prevents the normal G1/S transition. Cdc25A is required for the activation of the Cyclin E:Cdk2 complexes via dephosphorylation.<p>Chk1 is activated in response to DNA damage due to uv light. However, the phosphorylation occurs at a different site. inferred by electronic annotation IEA GO IEA Reactome Database ID Release 82 10349442 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10349442 Reactome R-SSC-69613 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-SSC-69613.1 The G1 arrest induced by DNA damage has been ascribed to the transcription factor and tumor suppressor protein p53. To be effective within minutes after DNA damage, induction of the G1 block should exploit transcription and protein synthesis independent mechanisms.<p>Upon exposure to ultraviolet light (UV) or ionizing radiation (IR), the abundance and activity of a protein, Cdc25A, rapidly decreases; this DNA damage response is not dependent on p53. The rapid destruction of Cdc25A phosphatase prevents entry of a cell into S-phase, by maintaining the CyclinE:Cdk2 complexes in their T14Y15 phosphorylated form. 10827953 Pubmed 2000 Rapid destruction of human Cdc25A in response to DNA damage. Mailand, N Lukas, C SyljuĆ¢sen, RG Welcker, M Lukas, J Science 288:1425-9 inferred by electronic annotation IEA GO IEA