BioPAX pathway converted from "DNA replication initiation" in the Reactome database.DNA replication initiationDNA replication initiationThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>2.7.7.6The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer at the originThe primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer at the originThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Converted from EntitySet in ReactomeReactome DB_ID: 305951nucleoplasmGO0005654NTP [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityGTP [nucleoplasm]CTP [nucleoplasm]UTP [nucleoplasm]ATP [nucleoplasm]Reactomehttp://www.reactome.orgChEBI15996ChEBI17677ChEBI15713ChEBI30616Reactome DB_ID: 104133391DNA polymerase alpha:primase:DNA polymerase alpha:origin complex [nucleoplasm]DNA polymerase alpha:primase:DNA polymerase alpha:origin complexReactome DB_ID: 104133371DNA polymerase epsilon:origin complex [nucleoplasm]DNA polymerase epsilon:origin complexReactome DB_ID: 104133351DNA polymerase epsilon [nucleoplasm]DNA polymerase epsilonReactome DB_ID: 104133291UniProt:F1P2L0Gallus gallusNCBI Taxonomy9031UniProtF1P2L0Chain Coordinates2EQUAL147EQUALReactome DB_ID: 104133251UniProt:Q5ZKQ6 POLE2POLE2POLE2RCJMB04_9k18FUNCTION Accessory component of the DNA polymerase epsilon complex (By similarity). Participates in DNA repair and in chromosomal DNA replication (By similarity).SUBUNIT Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4.MISCELLANEOUS In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.SIMILARITY Belongs to the DNA polymerase epsilon subunit B family.UniProtQ5ZKQ61EQUAL527EQUALReactome DB_ID: 104133221UniProt:E1C5P2UniProtE1C5P21EQUAL2286EQUALReactome DB_ID: 104133331UniProt:A0A1D5NY53UniProtA0A1D5NY532EQUAL117EQUALReactome Database ID Release 7510413335Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413335ReactomeR-GGA-684831Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68483.1Reactome DB_ID: 684191origin of replication [nucleoplasm]origin of replicationARSautonomously replicating sequenceoriginReactome Database ID Release 7510413337Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413337ReactomeR-GGA-684851Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68485.1Reactome DB_ID: 104133181DNA polymerase alpha:primase [nucleoplasm]DNA polymerase alpha:primaseReactome DB_ID: 104133041UniProt:A0A3Q2TZM6UniProtA0A3Q2TZM61EQUAL1462EQUALReactome DB_ID: 104133061Ghost homologue of POLA2 [nucleoplasm]Ghost homologue of POLA2Reactome DB_ID: 104133161PRIM1:PRIM2 [nucleoplasm]PRIM1:PRIM2Reactome DB_ID: 104133141UniProt:Q5ZM06PRIM2UniProtQ5ZM061EQUAL509EQUALReactome DB_ID: 104133101UniProt:A0A1D5P0T5UniProtA0A1D5P0T51EQUAL420EQUALReactome Database ID Release 7510413316Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413316ReactomeR-GGA-96688121Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-9668812.1Reactome Database ID Release 7510413318Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413318ReactomeR-GGA-685071Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68507.1Reactome Database ID Release 7510413339Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413339ReactomeR-GGA-685101Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68510.1Reactome DB_ID: 104133351Reactome DB_ID: 104133411RNA primer:origin duplex:DNA polymerase alpha:primase complex [nucleoplasm]RNA primer:origin duplex:DNA polymerase alpha:primase complexReactome DB_ID: 684221RNA primer [nucleoplasm]RNA primerReactome DB_ID: 104133181Reactome DB_ID: 684191Reactome Database ID Release 7510413341Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413341ReactomeR-GGA-684231Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68423.1PHYSIOL-LEFT-TO-RIGHTACTIVATIONReactome DB_ID: 10413339GO0003899GO molecular functionReactome Database ID Release 7510413342Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413342Reactome Database ID Release 7510413344Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413344ReactomeR-GGA-689131Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68913.1At the beginning of this reaction, 1 molecule of 'DNA polymerase alpha:primase:DNA polymerase alpha:origin complex', and 1 molecule of 'NTP' are present. At the end of this reaction, 1 molecule of 'DNA polymerase epsilon', and 1 molecule of 'RNA primer:origin duplex:DNA polymerase alpha:primase complex' are present.<br><br> This reaction takes place in the 'nucleus' and is mediated by the 'DNA-directed RNA polymerase activity' of 'DNA polymerase alpha:primase'.<br>6693436Pubmed1984DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha.Wang, TSHu, SZKorn, DJ Biol Chem 259:1854-65inferred by electronic annotationIEAGOIEA2.7.7.7The polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer at the originThe polymerase component of DNA polymerase alpha:primase synthesizes a 20-nucleotide primer at the originThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 296041dATP [ChEBI:16284]dATP2'-deoxyadenosine 5'-triphosphateDeoxyadenosine triphosphateDeoxyadenosine 5'-triphosphateChEBI16284Reactome DB_ID: 301851dCTP [ChEBI:16311]dCTP2'-Deoxycytidine 5'-triphosphateDeoxycytidine triphosphatedeoxycytidine 5'-triphosphateChEBI16311Reactome DB_ID: 298921dGTP [ChEBI:16497]dGTPDeoxyguanosine 5'-triphosphate2'-deoxyguanosine 5'-triphosphateDeoxyguanosine triphosphateChEBI16497Reactome DB_ID: 301871dTTP [ChEBI:18077]dTTPDeoxythymidine triphosphateTTPDeoxythymidine 5'-triphosphateChEBI18077Reactome DB_ID: 104133411Reactome DB_ID: 104134841RNA primer-DNA primer:origin duplex [nucleoplasm]RNA primer-DNA primer:origin duplexReactome DB_ID: 104133411Reactome DB_ID: 684241DNA primer [nucleoplasm]DNA primerReactome Database ID Release 7510413484Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413484ReactomeR-GGA-684251Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68425.1PHYSIOL-LEFT-TO-RIGHTACTIVATIONReactome DB_ID: 10413318GO0003887GO molecular functionReactome Database ID Release 7510413485Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413485Reactome Database ID Release 7510413487Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10413487ReactomeR-GGA-689501Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68950.1At the beginning of this reaction, 1 molecule of 'dTTP', 1 molecule of 'dGTP', 1 molecule of 'dATP', 1 molecule of 'RNA primer:origin duplex:DNA polymerase alpha:primase complex', and 1 molecule of 'dCTP' are present. At the end of this reaction, 1 molecule of 'RNA primer-DNA primer:origin duplex' is present.<br><br> This reaction takes place in the 'nucleus' and is mediated by the 'DNA-directed DNA polymerase activity' of 'DNA polymerase alpha:primase'.<br>6094569Pubmed1984Purification of a DNA polymerase-DNA primase complex from calf thymus glandsChang, L MRafter, EAugl, CBollum, F JJ. Biol. Chem. 259:14679-87inferred by electronic annotationIEAGOIEAReactome Database ID Release 7510487790Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10487790ReactomeR-GGA-689521Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-GGA-68952.1GO0006270GO biological processDNA polymerases are not capable of de novo DNA synthesis and require synthesis of a primer, usually by a DNA-dependent RNA polymerase (primase) to begin DNA synthesis. In eukaryotic cells, the primer is synthesized by DNA polymerase alpha:primase. First, the DNA primase portion of this complex synthesizes approximately 6-10 nucleotides of RNA primer and then the DNA polymerase portion synthesizes an additional 20 nucleotides of DNA (Frick & Richardson 2002; Wang et al 1984).11395402Pubmed2002DNA primases.Frick, DNRichardson, CCAnnu Rev Biochem 70:39-80inferred by electronic annotationIEAGOIEA