BioPAX pathway converted from "Formation of Incision Complex in GG-NER" in the Reactome database.Formation of Incision Complex in GG-NERFormation of Incision Complex in GG-NERThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>TFIIH binds GG-NER site to form a verification complexTFIIH binds GG-NER site to form a verification complexThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 104955901nucleoplasmGO0005654TFIIH [nucleoplasm]TFIIHReactome DB_ID: 104955681UniProt:Q9VPX4Tfb4Reactomehttp://www.reactome.orgDrosophila melanogasterNCBI Taxonomy7227UniProtQ9VPX4Chain Coordinates1EQUAL308EQUALReactome DB_ID: 104955631UniProt:Q02870hayUniProtQ028701EQUAL782EQUALReactome DB_ID: 104955781UniProt:Q9VUR1mrnUniProtQ9VUR11EQUAL462EQUALReactome DB_ID: 104955581UniProt:Q7KVP9XpdUniProtQ7KVP91EQUAL760EQUALReactome DB_ID: 104955731UniProt:Q9VNP8Ssl1UniProtQ9VNP81EQUAL395EQUALReactome DB_ID: 104955881UniProt:B7Z018Tfb5UniProtB7Z0181EQUAL71EQUALReactome DB_ID: 104955531CAK [nucleoplasm]CAKReactome DB_ID: 104955411UniProt:Q24216Cdk7UniProtQ242161EQUAL346EQUALReactome DB_ID: 104955511UniProt:Q7KPG8Mat1UniProtQ7KPG81EQUAL309EQUALReactome DB_ID: 104955461UniProt:O76513CycHUniProtO765131EQUAL323EQUALReactome Database ID Release 7510495553Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10495553ReactomeR-DME-692211Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-69221.1Reactome DB_ID: 104955831UniProt:Q960E8Tfb1UniProtQ960E81EQUAL548EQUALReactome Database ID Release 7510495590Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10495590ReactomeR-DME-1096341Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-109634.1Reactome DB_ID: 105460901Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:PAR-PARP1,PAR-PARP2 [nucleoplasm]Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:PAR-PARP1,PAR-PARP2Reactome DB_ID: 105432471PAR-PARP1,PAR-PARP2 dimers [nucleoplasm]PAR-PARP1,PAR-PARP2 dimersReactome DB_ID: 105432452UniProt:P35875ParpUniProtP35875L-glutamyl-5-poly(ADP-ribose) at unknown positionL-glutamyl-5-poly(ADP-ribose)2EQUAL1014EQUALReactome Database ID Release 7510543247Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10543247ReactomeR-DME-56517091Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5651709.1Reactome DB_ID: 105460881Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB [nucleoplasm]Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDBReactome DB_ID: 56881141Distorted dsDNA [nucleoplasm]Distorted dsDNAReactome DB_ID: 105460861Ub:XPC:RAD23:CETN2 [nucleoplasm]Ub:XPC:RAD23:CETN2Converted from EntitySet in ReactomeReactome DB_ID: 105384611Homologues of CETN2 [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityCG15158 [nucleoplasm]Dmel\CG17493 [nucleoplasm]UniProtQ8T415UniProtM9PGG8Reactome DB_ID: 105460841Ub:XPC [nucleoplasm]Ub:XPCReactome DB_ID: 105460821UniProt:Q24595XpcUniProtQ24595ubiquitinylated lysine at unknown positionubiquitinylated lysine [MOD:01148]2EQUAL940EQUALConverted from EntitySet in ReactomeReactome DB_ID: 104918371Ub [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityRpL40 [nucleoplasm]UBI-P63E 3 [nucleoplasm]UBI-P63E 2 [nucleoplasm]Ubi-p5E [nucleoplasm]UBI-P63E 1 [nucleoplasm]UniProtP18101UniProtP0CG69UniProtQ9W418Reactome Database ID Release 7510546084Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546084ReactomeR-DME-67829511Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-6782951.1Converted from EntitySet in ReactomeReactome DB_ID: 105460791RAD23 [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityRad23 [nucleoplasm]UniProtQ9V3W9Reactome Database ID Release 7510546086Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546086ReactomeR-DME-67829471Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-6782947.1Reactome DB_ID: 105460771PAR-UV-DDB [nucleoplasm]PAR-UV-DDBReactome DB_ID: 105432661UniProt:Q5BI50Cul4UniProtQ5BI501EQUAL759EQUALReactome DB_ID: 105460751DDB1:PAR-DDB2 [nucleoplasm]DDB1:PAR-DDB2Reactome DB_ID: 105460731Ghost homologue of PAR-DDB2 [nucleoplasm]Ghost homologue of PAR-DDB2Reactome DB_ID: 105432731UniProt:Q9XYZ5picUniProtQ9XYZ51EQUAL1140EQUALReactome Database ID Release 7510546075Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546075ReactomeR-DME-56966801Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5696680.1Reactome DB_ID: 104918571UniProt:Q9W5E1 Roc1aRoc1aRoc1aCG16982FUNCTION Core component of multiple SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Required for the specific SCF-dependent proteolysis of CI, but not that of ARM, suggesting that it also participates in the selection of substrates inside the SCF complex (PubMed:12062088). During early metamorphosis, part of the SCF-slmb complex that negatively regulates the InR/PI3K/TOR pathway to activate the pruning of unnecessary larval ddaC dendrites and mushroom body axons (PubMed:24068890). The SCF-slmb complex also regulates asymmetrical division of neuroblasts and inhibits ectopic neuroblast formation partly through SAK and Akt1 (PubMed:24413555). Also part of an SCF complex required for caspase activation during sperm differentiation (Probable).PATHWAY Protein modification; protein ubiquitination.SUBUNIT Component of SCF E3 ubiquitin-protein ligase complexes consisting of Skpa, Cul1, Roc1a and an F-box protein. In larvae neuroblast self renewal and asymmetric division, as well as ddaC dendrite and mushroom body axon pruning, the complex contains the F-box protein slmb (SCF-slmb) (PubMed:24068890, PubMed:24413555). Interacts directly with Cul1 and Slmb (PubMed:11500045). In caspase activation during sperm differentiation, the complex contains the F-box protein ntc (Probable).TISSUE SPECIFICITY Widely expressed. Expressed in embryonic, larval and adult tissues.DEVELOPMENTAL STAGE Expressed both maternally and zygotically.DOMAIN The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion (By similarity).DISRUPTION PHENOTYPE Severe pruning defect in ddaC neurons (PubMed:24068890). Also shows defects in ddaD and ddaE neuron pruning and in ddaF apoptosis (PubMed:24068890). RNAi-mediated knockdown results in apical detachment of scolopidial cells in Johnston's organ (PubMed:27331610).SIMILARITY Belongs to the RING-box family.UniProtQ9W5E12EQUAL108EQUALReactome Database ID Release 7510546077Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546077ReactomeR-DME-56966491Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5696649.1Reactome Database ID Release 7510546088Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546088ReactomeR-DME-56966591Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5696659.1Reactome Database ID Release 7510546090Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546090ReactomeR-DME-56966811Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5696681.1Reactome DB_ID: 105460921Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:TFIIH:PAR-PARP1,PAR-PARP2 [nucleoplasm]Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:TFIIH:PAR-PARP1,PAR-PARP2Reactome DB_ID: 104955901Reactome DB_ID: 105460901Reactome Database ID Release 7510546092Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546092ReactomeR-DME-56898621Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5689862.1Reactome Database ID Release 7510546725Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546725ReactomeR-DME-56910001Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5691000.1GO0006294GO biological processTranscription factor II H (TFIIH) complex is recruited to DNA damage sites after the damage is recognized by the XPC:RAD23:CETN2 complex and the UV-DDB complex (DDB1:DDB2) (Volker et al. 2001, Araujo and Wood 1999).<p>TFIIH consists of ten subunits organized into a ring-like structure (Schultz et al. 2000). The TFIIH core, also forming a ring-like structure, includes a DNA helicase ERCC3 (XPB), GTF2H1 (BTF2-p62), GTF2H2 (BTF2-p44), GTF2H3 (BTF2-p34) and GTF2H4 (BTF2-p52). GTF2H4 directly interacts with ERCC3 and anchors it to the TFIIH complex (Jawhari et al. 2002). Another DNA helicase, ERCC2 (XPD) is anchored to the TFIIH complex by binding to the GTF2H2 subunit (Coin et al. 1998). The CDK-activating kinase (CAK) complex, consisting of CCNH (cyclin H), CDK7 and MNAT1 (MAT1) is included in the TFIIH complex through an interaction with ERCC2 (Reardon et al. 1996, Rossignol et al. 1997). The tenth subunit, GTF2H5 (TTDA, TFB5, BTF2-p5) is important for the stability of the TFIIH complex (Giglia-Mari et al. 2004). The TFIIH complex binds the DNA damage site after XPC:RAD23:CETN2 complex recognizes the damage (Volker et al. 2001, Riedl et al. 2003), and the ERCC3 and GTF2H1 subunits of TFIIH directly interact with XPC (Yokoi et al. 2003).11511374Pubmed2001Sequential assembly of the nucleotide excision repair factors in vivo.Volker, MMoné, MJKarmakar, Pvan Hoffen, ASchul, WVermeulen, WHoeijmakers, Jan H Jvan Driel, Rvan Zeeland, AAMullenders, LHMol Cell 8:213-249130708Pubmed1997Substrate specificity of the cdk-activating kinase (CAK) is altered upon association with TFIIHRossignol, MKolb-Cheynel, IEgly, JMEMBO J. 16:1628-3725154395Pubmed2014Sequential and ordered assembly of a large DNA repair complex on undamaged chromatinZiani, SalimNagy, ZitaAlekseev, SergeySoutoglou, EviEgly, Jean-MarcCoin, FredericJ. Cell Biol. 206:589-9815220921Pubmed2004A new, tenth subunit of TFIIH is responsible for the DNA repair syndrome trichothiodystrophy group AGiglia-Mari, GiuseppinaCoin, FredericRanish, Jeffrey AHoogstraten, DeborahTheil, ArjanWijgers, NilsJaspers, Nicolaas G JRaams, AnjaArgentini, Manuelavan der Spek, P JBotta, ElenaStefanini, MiriaEgly, Jean-MarcAebersold, RuediHoeijmakers, Jan H JVermeulen, WimNat. Genet. 36:714-911007478Pubmed2000Molecular structure of human TFIIHSchultz, PFribourg, SPoterszman, AMallouh, VMoras, DEgly, JMCell 102:599-60712080057Pubmed2002p52 Mediates XPB function within the transcription/repair factor TFIIHJawhari, AnassLainé, Jean-PhilippeDubaele, SandyLamour, VPoterszman, ArnaudCoin, FredericMoras, DinoEgly, Jean-MarcJ. Biol. Chem. 277:31761-79771713Pubmed1998Mutations in the XPD helicase gene result in XP and TTD phenotypes, preventing interaction between XPD and the p44 subunit of TFIIHCoin, FMarinoni, J CRodolfo, CFribourg, SPedrini, A MEgly, JMNat. Genet. 20:184-88692841Pubmed1996Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIHReardon, J TGe, HGibbs, ESancar, AHurwitz, JPan, ZQProc. Natl. Acad. Sci. U.S.A. 93:6482-714517266Pubmed2003The comings and goings of nucleotide excision repair factors on damaged DNARiedl, ThiloHanaoka, FumioEgly, Jean-MarcEMBO J. 22:5293-30310734143Pubmed2000The xeroderma pigmentosum group C protein complex XPC-HR23B plays an important role in the recruitment of transcription factor IIH to damaged DNAYokoi, MMasutani, CMaekawa, TSugasawa, KOhkuma, YHanaoka, FJ. Biol. Chem. 275:9870-510526214Pubmed1999Protein complexes in nucleotide excision repair.Araujo, SJWood, Richard DMutat Res 435:23-33inferred by electronic annotationIEAGOIEARecruitment of XPA and release of CAK Recruitment of XPA and release of CAK This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 105460921Reactome DB_ID: 105460711UniProt:P28518XpacUniProtP285182EQUAL273EQUALReactome DB_ID: 105460961Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1,PAR-PARP2 [nucleoplasm]Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1,PAR-PARP2Reactome DB_ID: 105460901Reactome DB_ID: 1054607112EQUAL273EQUALReactome DB_ID: 105460941TFIIH Core [nucleoplasm]TFIIH CoreReactome DB_ID: 1049556811EQUAL308EQUALReactome DB_ID: 1049556311EQUAL782EQUALReactome DB_ID: 1049557811EQUAL462EQUALReactome DB_ID: 1049555811EQUAL760EQUALReactome DB_ID: 1049557311EQUAL395EQUALReactome DB_ID: 1049558811EQUAL71EQUALReactome DB_ID: 1049558311EQUAL548EQUALReactome Database ID Release 7510546094Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546094ReactomeR-DME-56896241Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5689624.1Reactome Database ID Release 7510546096Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546096ReactomeR-DME-56893001Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5689300.1Reactome DB_ID: 104955531Reactome Database ID Release 7510546098Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546098ReactomeR-DME-56898611Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5689861.1XPA binds the DNA damage site through interaction with the TFIIH complex subunit GTF2H5 (TTDA) (Ziani et al. 2014), and also interacts with the DDB2 subunit of the UV-DDB complex (Wakasugi et al. 2001, Wakasugi et al. 2009, Takedachi et al. 2010). PARylated PARP1 (or possibly PARP2) (King et al. 2012) facilitates XPA association with chromatin. Binding of XPA is accompanied by the release of the CAK subcomplex from the TFIIH complex (Coin et al. 2008).11278856Pubmed2001Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein AWakasugi, MShimizu, MMorioka, HLinn, SNikaido, OMatsunaga, TJ. Biol. Chem. 276:15434-4020368362Pubmed2010DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPATakedachi, AratoSaijo, MasafumiTanaka, KiyojiMol. Cell. Biol. 30:2708-2318614043Pubmed2008Nucleotide excision repair driven by the dissociation of CAK from TFIIHCoin, FredericOksenych, ValentynMocquet, VincentGroh, StefanieBlattner, ChristineEgly, Jean MarcMol. Cell 31:9-2019056823Pubmed2009Physical and functional interaction between DDB and XPA in nucleotide excision repairWakasugi, MitsuoKasashima, HiromiFukase, YukoImura, MayumiImai, RikaYamada, SakiCleaver, James EMatsunaga, TsukasaNucleic Acids Res. 37:516-2523038248Pubmed2012Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repairKing, Brenee SCooper, Karen LLiu, Ke JianHudson, Laurie GJ. Biol. Chem. 287:39824-33inferred by electronic annotationIEAGOIEAERCC2 and ERCC3 DNA helicases form an open bubble structure in damaged DNAERCC2 and ERCC3 DNA helicases form an open bubble structure in damaged DNAThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 293581ATP(4-) [ChEBI:30616]ATP(4-)Adenosine 5'-triphosphateatpATPChEBI30616Reactome DB_ID: 105467141Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1,PAR-PARP2:CHD1L [nucleoplasm]Ub:XPC:RAD23:CETN2:Distorted dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1,PAR-PARP2:CHD1LReactome DB_ID: 105460961Reactome DB_ID: 105464771Ghost homologue of CHD1L [nucleoplasm]Ghost homologue of CHD1LReactome Database ID Release 7510546714Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546714ReactomeR-DME-56966521Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5696652.1Reactome DB_ID: 105467181Ub:XPC:RAD23:CETN2:Open bubble-dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1,PAR-PARP2:CHD1L [nucleoplasm]Ub:XPC:RAD23:CETN2:Open bubble-dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1,PAR-PARP2:CHD1LReactome DB_ID: 105467161Ub:XPC:RAD23:CETN2:Open bubble-dsDNA:PAR-UV-DDB [nucleoplasm]Ub:XPC:RAD23:CETN2:Open bubble-dsDNA:PAR-UV-DDBReactome DB_ID: 1099441damaged DNA with open bubble structure [nucleoplasm]damaged DNA with open bubble structureReactome DB_ID: 105460861Reactome DB_ID: 105460771Reactome Database ID Release 7510546716Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546716ReactomeR-DME-56892971Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5689297.1Reactome DB_ID: 105432471Reactome DB_ID: 105464771Reactome DB_ID: 1054607112EQUAL273EQUALReactome DB_ID: 105460941Reactome Database ID Release 7510546718Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546718ReactomeR-DME-56910181Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5691018.1Reactome DB_ID: 1135501hydrogenphosphate [ChEBI:43474]hydrogenphosphate[PO3(OH)](2-)HYDROGENPHOSPHATE IONhydrogen phosphate[P(OH)O3](2-)HPO4(2-)phosphateINORGANIC PHOSPHATE GROUPChEBI43474Reactome DB_ID: 1135821ADP(3-) [ChEBI:456216]ADP(3-)ADP trianion5&apos;-O-[(phosphonatooxy)phosphinato]adenosineADPChEBI456216PHYSIOL-LEFT-TO-RIGHTACTIVATIONReactome DB_ID: 10546714GO0004386GO molecular functionReactome Database ID Release 7510546719Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546719Reactome Database ID Release 7510546721Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546721ReactomeR-DME-56909961Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5690996.1GO0000717GO biological processTwo DNA helicases XPB (ERCC3) and XPD (ERCC2), which are part of the TFIIH complex, unwind the distorted DNA duplex around the lesion to form an open bubble structure that exposes the damaged site. The helicase activity of the TFIIH complex is stimulated by the presence of XPA and the XPC:RAD23:CETN2 complex (Winkler et al. 2001). The 5'->3' directed helicase activity of ERCC2 (Kuper et al. 2012) is crucial for unwinding of the distorted dsDNA during nucleotide excision repair (NER) (Coin et al. 2007). The 3'->5' directed DNA helicase ERCC3 contributes to dsDNA unwinding during NER through ATP hydrolysis (Coin et al. 2007). In addition to DNA unwinding, ERCC2 and ERCC3 verify the presence of DNA damage (Oksenych et al. 2009, Mathieu et al. 2010, Mathieu et al. 2013). Verification of DNA damage also involves XPA (Camenisch et al. 2006). The binding site of XPC determines which DNA strand is selected by ERCC2 to verify the presence of lesions (Sugasawa et al. 2009).22081108Pubmed2012Functional and structural studies of the nucleotide excision repair helicase XPD suggest a polarity for DNA translocationKuper, JochenWolski, Stefanie CMichels, GudrunKisker, CarolineEMBO J. 31:494-50219713942Pubmed2009Molecular insights into the recruitment of TFIIH to sites of DNA damageOksenych, ValentynBernardes de Jesus, BrunoZhovmer, AlexanderEgly, Jean-MarcCoin, FredericEMBO J. 28:2971-8011141066Pubmed2001Novel functional interactions between nucleotide excision DNA repair proteins influencing the enzymatic activities of TFIIH, XPG, and ERCC1-XPFWinkler, G SSugasawa, KEker, A Pde Laat, W LHoeijmakers, Jan H JBiochemistry 40:160-519941824Pubmed2009Two-step recognition of DNA damage for mammalian nucleotide excision repair: Directional binding of the XPC complex and DNA strand scanningSugasawa, KaoruAkagi, Jun-IchiNishi, RyotaroIwai, ShigenoriHanaoka, FumioMol. Cell 36:642-5317466626Pubmed2007Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repairCoin, FredericOksenych, ValentynEgly, Jean-MarcMol. Cell 26:245-5620876134Pubmed2010Strand- and site-specific DNA lesion demarcation by the xeroderma pigmentosum group D helicaseMathieu, NadineKaczmarek, NinaNaegeli, HanspeterProc. Natl. Acad. Sci. U.S.A. 107:17545-5023352696Pubmed2013DNA quality control by a lesion sensor pocket of the xeroderma pigmentosum group D helicase subunit of TFIIHMathieu, NadineKaczmarek, NinaRüthemann, PeterLuch, AndreasNaegeli, HanspeterCurr. Biol. 23:204-1216491090Pubmed2006Recognition of helical kinks by xeroderma pigmentosum group A protein triggers DNA excision repairCamenisch, UlrikeDip, RamiroSchumacher, Sylvie BriandSchuler, BenjaminNaegeli, HanspeterNat. Struct. Mol. Biol. 13:278-84inferred by electronic annotationIEAGOIEASUMOylation of XPCSUMOylation of XPCThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 105467181Converted from EntitySet in ReactomeReactome DB_ID: 105494261SUMO1,2,3:UBE2I [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityReactome DB_ID: 105494351Ub,SUMO:XPC:RAD23:CETN2:Open bubble-dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1, PAR-PARP2:CHD1L [nucleoplasm]Ub,SUMO:XPC:RAD23:CETN2:Open bubble-dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1, PAR-PARP2:CHD1LReactome DB_ID: 105494331Ub,SUMO:XPC:RAD23:CETN2 [nucleoplasm]Ub,SUMO:XPC:RAD23:CETN2Converted from EntitySet in ReactomeReactome DB_ID: 105384611Converted from EntitySet in ReactomeReactome DB_ID: 105460791Reactome DB_ID: 105494311Ub,SUMO:XPC [nucleoplasm]Ub,SUMO:XPCReactome DB_ID: 105355761UniProt:O97102smt3UniProtO971021EQUAL93EQUALReactome DB_ID: 105494291ubiquitinylated lysine at unknown positionsumoylated lysine at unknown positionsumoylated lysine2EQUAL940EQUALConverted from EntitySet in ReactomeReactome DB_ID: 104918371Reactome Database ID Release 7510549431Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10549431ReactomeR-DME-67904621Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-6790462.1Reactome Database ID Release 7510549433Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10549433ReactomeR-DME-67904611Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-6790461.1Reactome DB_ID: 1099441Reactome DB_ID: 105432471Reactome DB_ID: 105464771Reactome DB_ID: 105460771Reactome DB_ID: 1054607112EQUAL273EQUALReactome DB_ID: 105460941Reactome Database ID Release 7510549435Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10549435ReactomeR-DME-67904681Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-6790468.1Reactome DB_ID: 105356511UniProt:Q7KNM2lwrUniProtQ7KNM21EQUAL158EQUALPHYSIOL-LEFT-TO-RIGHTACTIVATIONConverted from EntitySet in ReactomeReactome DB_ID: 10549437PIAS1,3 [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityGO0019789GO molecular functionReactome Database ID Release 7510549438Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10549438Reactome Database ID Release 7510549440Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10549440ReactomeR-DME-67904541Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-6790454.1XPC undergoes SUMOylation following UV irradiation on several consensus SUMOylation sites (van Cuijk et al. 2015). SUMOylation of XPC probably succeeds the UV-DDB mediated ubiquitination of XPC, as the presence of both DDB2 and XPA is required for SUMOylation (Wang et al. 2005), but it has also been reported that SUMOylation of XPC was DDB2-independent (Akita et al. 2015). It is unclear whether XPC is modified by SUMO1 (Wang et al. 2005, Akita et al. 2015) or poly-SUMO2/3 (Poulsen et al. 2013). SUMO conjugases PIAS1 and PIAS3 both interact with XPC and may catalyze XPC SUMOylation (Akita et al. 2015).26042670Pubmed2015SUMOylation of xeroderma pigmentosum group C protein regulates DNA damage recognition during nucleotide excision repairAkita, MasakiTak, Yon-SooShimura, TsutomuMatsumoto, SyotaOkuda-Shimizu, YukiShimizu, YuichiroNishi, RyotaroSaitoh, HisatoIwai, ShigenoriMori, ToshioIkura, TsuyoshiSakai, WataruHanaoka, FumioSugasawa, KaoruSci Rep 5:1098423751493Pubmed2013RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage responsePoulsen, Sara LHansen, Rebecca KWagner, Sebastian Avan Cuijk, Loesvan Belle, Gijsbert JStreicher, WernerWikström, MatsChoudhary, ChunaramHoutsmuller, Adriaan BMarteijn, Jurgen ABekker-Jensen, SimonMailand, NielsJ. Cell Biol. 201:797-80726151477Pubmed2015SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repairvan Cuijk, Loesvan Belle, Gijsbert JTurkyilmaz, YaseminPoulsen, Sara LJanssens, Roel CTheil, Arjan FSabatella, MariangelaLans, HannesMailand, NielsHoutsmuller, Adriaan BVermeulen, WimMarteijn, Jurgen ANat Commun 6:749916030353Pubmed2005DNA repair factor XPC is modified by SUMO-1 and ubiquitin following UV irradiationWang, Qi-EnZhu, QianzhengWani, GulzarEl-Mahdy, Mohamed ALi, JinyouWani, Altaf ANucleic Acids Res. 33:4023-34inferred by electronic annotationIEAGOIEAFormation of ERCC1:XPF heterodimeric complexFormation of ERCC1:XPF heterodimeric complexERCC1 binds ERCC4 (XPF)This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 104981941UniProt:Q24087mei-9UniProtQ240871EQUAL916EQUALReactome DB_ID: 104981891UniProt:Q7KMG7Ercc1UniProtQ7KMG71EQUAL297EQUALReactome DB_ID: 104981961ERCC1:ERCC4 [nucleoplasm]ERCC1:ERCC4Reactome DB_ID: 1049819411EQUAL916EQUALReactome DB_ID: 1049818911EQUAL297EQUALReactome Database ID Release 7510498196Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10498196ReactomeR-DME-1099431Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-109943.1Reactome Database ID Release 7510498198Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10498198ReactomeR-DME-1099551Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-109955.1ERCC1 binds ERCC4 (XPF) to form a heterodimeric ERCC1:ERCC4 (ERCC1:XPF) complex with a DNA endonuclease activity, where ERCC4 is the catalytic subunit. Suitable substrates for the ERCC1:ERCC4 endonuclease are single strand DNA (ssDNA) and ssDNA region of a duplex DNA with an open bubble structure (Park et al. 1995). 7559382Pubmed1995Purification and characterization of the XPF-ERCC1 complex of human DNA repair excision nucleasePark, C HBessho, TMatsunaga, TSancar, AJ. Biol. Chem. 270:22657-60inferred by electronic annotationIEAGOIEABinding of ERCC1:ERCC4 (ERCC1:XPF) to pre-incision complex in GG-NERBinding of ERCC1:ERCC4 (ERCC1:XPF) to pre-incision complex in GG-NERThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>Reactome DB_ID: 105467051GG-NER pre-incision complex:Open bubble-dsDNA [nucleoplasm]GG-NER pre-incision complex:Open bubble-dsDNAReactome DB_ID: 105464751Ghost homologue of ERCC5 [nucleoplasm]Ghost homologue of ERCC5Reactome DB_ID: 104920711RPA heterotrimer [nucleoplasm]RPA heterotrimerReactome DB_ID: 104917341UniProt:Q9VIH1RPA2UniProtQ9VIH11EQUAL270EQUALReactome DB_ID: 104917271UniProt:Q9VYW3RPA3UniProtQ9VYW31EQUAL121EQUALReactome DB_ID: 104917221Ghost homologue of RPA1 [nucleoplasm]Ghost homologue of RPA1Reactome Database ID Release 7510492071Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10492071ReactomeR-DME-684621Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-68462.1Reactome DB_ID: 105467031Open bubble-dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1, PAR-PARP2:CHD1L [nucleoplasm]Open bubble-dsDNA:PAR-UV-DDB:TFIIH Core:XPA:PAR-PARP1, PAR-PARP2:CHD1LReactome DB_ID: 1099441Reactome DB_ID: 105432471Reactome DB_ID: 105464771Reactome DB_ID: 105460771Reactome DB_ID: 1054607112EQUAL273EQUALReactome DB_ID: 105460941Reactome Database ID Release 7510546703Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546703ReactomeR-DME-67905171Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-6790517.1Reactome Database ID Release 7510546705Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546705ReactomeR-DME-56910431Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5691043.1Reactome DB_ID: 104981961Reactome DB_ID: 105467071GG-NER incision complex:Open bubble dsDNA [nucleoplasm]GG-NER incision complex:Open bubble dsDNAReactome DB_ID: 105467051Reactome DB_ID: 104981961Reactome Database ID Release 7510546707Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546707ReactomeR-DME-56910461Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5691046.1Reactome Database ID Release 7510546712Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10546712ReactomeR-DME-56909911Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5690991.1GO0006293GO biological processERCC1:ERCC4 (ERCC1:XPF) DNA endonuclease complex binds 5' to the DNA damage at global genome nucleotide excision repair (GG-NER) sites to form the incision complex. Binding of ERCC5 (XPG) to the NER site precedes the recruitment of ERCC1:ERCC4 (Riedl et al. 2003). ERCC1 directly interacts with XPA, and this interaction is necessary for the loading of ERCC1:ERCC4 to the open bubble structure in damaged dsDNA and the progression of GG-NER (Tsodikov et al. 2007, Orelli et al. 2010).17948053Pubmed2007Structural basis for the recruitment of ERCC1-XPF to nucleotide excision repair complexes by XPATsodikov, Oleg VIvanov, DmitriOrelli, BarbaraStaresincic, LidijaShoshani, IlanaOberman, RobertSchärer, Orlando DWagner, GerhardEllenberger, TomEMBO J. 26:4768-7619940136Pubmed2010The XPA-binding domain of ERCC1 is required for nucleotide excision repair but not other DNA repair pathwaysOrelli, BarbaraMcClendon, T BrookeTsodikov, Oleg VEllenberger, TomNiedernhofer, Laura JSchärer, Orlando DJ. Biol. Chem. 285:3705-12inferred by electronic annotationIEAGOIEAReactome Database ID Release 7510567621Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10567621ReactomeR-DME-56963951Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DME-5696395.1After the XPC complex and the UV-DDB complex bind damaged DNA, a basal transcription factor TFIIH is recruited to the nucleotide excision repair (NER) site (Volker et al. 2001, Riedl et al. 2003). DNA helicases ERCC2 (XPD) and ERCC3 (XPB) are subunits of the TFIIH complex. ERCC2 unwinds the DNA around the damage in concert with the ATPase activity of ERCC3, creating an open bubble (Coin et al. 2007). Simultaneously, the presence of the damage is verified by XPA (Camenisch et al. 2006). The recruitment of XPA is partially regulated by PARP1 and/or PARP2 (King et al. 2012).<p>Two DNA endonucleases, ERCC5 (XPG) and the complex of ERCC1 and ERCC4 (XPF), are recruited to the open bubble structure to form the incision complex that will excise the damaged oligonucleotide from the affected DNA strand (Dunand-Sauthier et al. 2005, Zotter et al. 2006, Riedl et al. 2003, Tsodikov et al. 2007, Orelli et al. 2010). The RPA heterotrimer coats the undamaged DNA strand, thus protecting it from the endonucleolytic attack (De Laat et al. 1998).15590680Pubmed2005The spacer region of XPG mediates recruitment to nucleotide excision repair complexes and determines substrate specificityDunand-Sauthier, IsabelleHohl, MarcelThorel, FabrizioJaquier-Gubler, PascaleClarkson, Stuart GSchärer, Orlando DJ. Biol. Chem. 280:7030-79716411Pubmed1998DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repairde Laat, W LAppeldoorn, ESugasawa, KWeterings, EJaspers, N GHoeijmakers, Jan H JGenes Dev. 12:2598-60917000769Pubmed2006Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIHZotter, AngelikaLuijsterburg, Martijn SWarmerdam, Daniël OIbrahim, ShehuNigg, Alexvan Cappellen, Wiggert AHoeijmakers, Jan H Jvan Driel, RoelVermeulen, WimHoutsmuller, Adriaan BMol. Cell. Biol. 26:8868-79inferred by electronic annotationIEAGOIEA