BioPAX pathway converted from "Activation of conventional Protein Kinase C" in the Reactome database. Activation of conventional Protein Kinase C Activation of conventional Protein Kinase C The conventional Protein Kinase C (cPKC) isoforms have two membrane-targetting domains, a C1 domain which binds to the membrane lipid diacylglycerol (DAG) and a C2 domain which binds membrane phospholipids such as phosphatidylserine, in a calcium-dependent manner. Association of both domains with the plasma membrane produces a conformational change that releases an autoinhibitory pseudosubstrate segment from the substrate-binding cavity, allowing substrate binding and downstream signaling. Protein Kinase C (PKC) is positively regulated by events that increase the plasma membrane concentration of diacylglycerol (DAG). Activation of PKC requires the coordinated binding of two membrane-targeting domains. The C1 domain binds diacylglycerol, the C2 domain binds phosphatidylserine. Each can bind the membrane independently, but with insufficient affinity for membrane recruitment and activation. Reactome DB_ID: 114519 1 plasma membrane GO 0005886 1,2-diacyl-sn-glycerol [ChEBI:17815] 1,2-diacyl-sn-glycerol Reactome http://www.reactome.org ChEBI 17815 Reactome DB_ID: 74016 1 cytosol GO 0005829 calcium(2+) [ChEBI:29108] calcium(2+) ChEBI 29108 Converted from EntitySet in Reactome Reactome DB_ID: 114528 1 Protein Kinase C, conventional [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity PRKCB [cytosol] PRKCA [cytosol] PRKCG [cytosol] Homo sapiens NCBI Taxonomy 9606 UniProt P05771 UniProt P17252 UniProt P05129 Reactome DB_ID: 33625 1 3-sn-phosphatidyl-L-serine [ChEBI:11750] 3-sn-phosphatidyl-L-serine L-alpha-phosphatidylserine 3-sn-phosphatidyl-L-serines 3-L-phosphatidyl-L-serine 1-D-phosphatidyl-L-serine 1,2-diacyl-sn-glycerol 3-phospho-L-serine 3-O-sn-phosphatidyl-L-serine ChEBI 11750 Converted from EntitySet in Reactome Reactome DB_ID: 139830 1 Activated conventional protein kinase C [plasma membrane] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Reactome Database ID Release 82 114553 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114553 Reactome R-HSA-114553 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-114553.4 9780306478635 ISBN 2004 Protein Kinase C 2nd ed Dekker, L Protein Kinase C 2nd ed (Book) 9814702 Pubmed 1998 Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals Oancea, E Meyer, T Cell 95:307-18 19033211 Pubmed 2009 Lipid activation of protein kinases Newton, AC J Lipid Res 50:S266-71 9601053 Pubmed 1998 The extended protein kinase C superfamily Mellor, H Parker, PJ Biochem J 332:281-92 ACTIVATION Reactome Database ID Release 82 425851 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=425851 Reactome R-HSA-425851 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-425851.1 Reactome DB_ID: 114519