BioPAX pathway converted from "Arachidonic acid is epoxidated to 8,9/11,12/14,15-EET by CYP(5)" in the Reactome database. Arachidonic acid is epoxidated to 8,9/11,12/14,15-EET by CYP(5) Arachidonic acid is epoxidated to 8,9/11,12/14,15-EET by CYP(5) Several cytochrome P450s (CYPs) convert arachidonic acid to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (8,9-, 11,12-, 14,15-EETs). The CYPs and their references are as follows: CYP1A1, CYP1A2, CYP1B1 (Choudhary et al. 2004); CYP2C8, CYP2C9 (Rifkind et al. 1995); CYP2C19 (Bylund et al. 1998, Rifkind et al. 1995); CYP2J2 (Wu et al. 1996). Authored: Williams, MG, 2012-02-24 Reviewed: Rush, MG, 2012-11-10 Edited: Williams, MG, 2012-02-24 Reactome DB_ID: 70106 1 cytosol GO 0005829 hydron [ChEBI:15378] hydron Reactome http://www.reactome.org ChEBI 15378 Reactome DB_ID: 29368 1 dioxygen [ChEBI:15379] dioxygen ChEBI 15379 Reactome DB_ID: 29364 1 NADPH(4-) [ChEBI:57783] NADPH(4-) NADPH 2'-O-phosphonatoadenosine 5'-{3-[1-(3-carbamoyl-1,4-dihydropyridin-1-yl)-1,4-anhydro-D-ribitol-5-yl] diphosphate} NADPH tetraanion ChEBI 57783 Reactome DB_ID: 29768 1 arachidonate [ChEBI:32395] arachidonate (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate (20:4n6) (5Z,8Z,11Z,14Z)-eicosatetraenoate ChEBI 32395 Converted from EntitySet in Reactome Reactome DB_ID: 2161753 1 8,9/11,12/14,15-EET [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity 11,12-EET [cytosol] 14,15-EET [cytosol] 8,9-EET [cytosol] ChEBI 34130 ChEBI 34157 ChEBI 34490 Reactome DB_ID: 29356 1 water [ChEBI:15377] water ChEBI 15377 Reactome DB_ID: 29366 1 NADP(3-) [ChEBI:58349] NADP(3-) NADP(+) 2'-O-phosphonatoadenosine 5'-{3-[1-(3-carbamoylpyridinio)-1,4-anhydro-D-ribitol-5-yl] diphosphate} NADP trianion ChEBI 58349 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Converted from EntitySet in Reactome Reactome DB_ID: 2161990 endoplasmic reticulum membrane GO 0005789 CYP(5) [endoplasmic reticulum membrane] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity CYP2C9 [endoplasmic reticulum membrane] CYP1A1 [endoplasmic reticulum membrane] CYP2C19 [endoplasmic reticulum membrane] CYP1A2 [endoplasmic reticulum membrane] CYP1B1 [endoplasmic reticulum membrane] CYP2C8 [endoplasmic reticulum membrane] CYP2J2 [endoplasmic reticulum membrane] Homo sapiens NCBI Taxonomy 9606 UniProt P11712 UniProt P04798 UniProt P33261 UniProt P05177 UniProt Q16678 UniProt P10632 UniProt P51589 GO 0004497 GO molecular function Reactome Database ID Release 82 2161882 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2161882 Reactome Database ID Release 82 2161899 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2161899 Reactome R-HSA-2161899 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-2161899.2 9866708 Pubmed 1998 Analysis of cytochrome P450 metabolites of arachidonic and linoleic acids by liquid chromatography-mass spectrometry with ion trap MS Bylund, J Ericsson, J Oliw, EH Anal Biochem 265:55-68 8631948 Pubmed 1996 Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart Wu, S Moomaw, CR Tomer, KB Falck, JR Zeldin, DC J Biol Chem 271:3460-8 7625847 Pubmed 1995 Arachidonic acid metabolism by human cytochrome P450s 2C8, 2C9, 2E1, and 1A2: regioselective oxygenation and evidence for a role for CYP2C enzymes in arachidonic acid epoxygenation in human liver microsomes Rifkind, AB Lee, C Chang, TK Waxman, DJ Arch Biochem Biophys 320:380-9 15258110 Pubmed 2004 Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1 Choudhary, D Jansson, I Stoilov, I Sarfarazi, M Schenkman, JB Drug Metab Dispos 32:840-7