BioPAX pathway converted from "LC3 de-lipidation by ATG4" in the Reactome database. 3.4.22.41 3.4.22.40 3.4.22.34 3.4.22.8 3.4.22.28 3.4.22.16 3.4.22.27 3.4.22.38 3.4.22.1 3.4.22.15 3.4.22.14 LC3 de-lipidation by ATG4 LC3 de-lipidation by ATG4 LC3:PE can be cleaved by ATG4 to release free LC3 (Kirisako et al. 2000, Kabeya et al. 2004, Kumanomidou et al. 2006, Satoo et al. 2009). ATG4 activity can be regulated by reactive oxygen species (ROS) (Scherz-Shouval et al. 2007) possibily to control release of LC3 from closed autophagosomes. Authored: Jupe, Steve, 2015-02-02 Reviewed: Tooze, Sharon A, 2015-05-13 Reviewed: Klionsky, Daniel J, 2015-09-03 Edited: Jupe, Steve, 2015-05-13 Reactome DB_ID: 5682873 1 phagophore assembly site membrane GO 0034045 LC3:PE [phagophore assembly site membrane] LC3:PE Reactome DB_ID: 5681998 1 phosphatidylethanolamine zwitterion [ChEBI:57613] phosphatidylethanolamine zwitterion 1,2-diacylglycero-3-phosphoethanolamine Reactome http://www.reactome.org ChEBI 57613 Converted from EntitySet in Reactome Reactome DB_ID: 5682694 1 cytosol GO 0005829 LC3 family [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity GABARAP [cytosol] MAP1LC3C [cytosol] MAP1LC3A [cytosol] GABARAPL2 [cytosol] MAP1LC3B [cytosol] GABARAPL1 [cytosol] Homo sapiens NCBI Taxonomy 9606 UniProt O95166 UniProt Q9BXW4 UniProt Q9H492 UniProt P60520 UniProt Q9GZQ8 UniProt Q9H0R8 Reactome Database ID Release 81 5682873 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=5682873 Reactome R-HSA-5682873 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-5682873.2 Reactome DB_ID: 5681998 1 Converted from EntitySet in Reactome Reactome DB_ID: 5682694 1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Converted from EntitySet in Reactome Reactome DB_ID: 5682664 ATG4 [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity ATG4B [cytosol] UniProt Q9Y4P1 GO 0004197 GO molecular function Reactome Database ID Release 81 5682649 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=5682649 Reactome Database ID Release 81 5682377 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=5682377 Reactome R-HSA-5682377 5 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-5682377.5 15169837 Pubmed 2004 LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation Kabeya, Yukiko Mizushima, Noboru Yamamoto, A Oshitani-Okamoto, Satsuki Ohsumi, Yoshinori Yoshimori, Tamotsu J. Cell. Sci. 117:2805-12 19322194 Pubmed 2009 The structure of Atg4B-LC3 complex reveals the mechanism of LC3 processing and delipidation during autophagy Satoo, Kenji Noda, Nobuo N Kumeta, Hiroyuki Fujioka, Yuko Mizushima, Noboru Ohsumi, Yoshinori Inagaki, F EMBO J. 28:1341-50 11038174 Pubmed 2000 The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway Kirisako, T Ichimura, Y Okada, H Kabeya, Y Mizushima, N Yoshimori, T Ohsumi, M Takao, T Noda, T Ohsumi, Y J. Cell Biol. 151:263-76 17347651 Pubmed 2007 Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4 Scherz-Shouval, Ruth Shvets, Elena Fass, Ephraim Shorer, Hagai Gil, Lidor Elazar, Zvulun EMBO J. 26:1749-60 16325851 Pubmed 2006 The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers Kumanomidou, Taichi Mizushima, Tsunehiro Komatsu, Masaaki Suzuki, Atsuo Tanida, Isei Sou, Yu-shin Ueno, Takashi Kominami, Eiki Tanaka, Keiji Yamane, Takashi J. Mol. Biol. 355:612-8