BioPAX pathway converted from "IGF2BP1 binds MAPK4 mRNA" in the Reactome database.IGF2BP1 binds MAPK4 mRNAIGF2BP1 binds MAPK4 mRNAIGF2BP1 is a cytosolic RNA-binding protein that recruits target transcripts to RNP particles for storage or transport. These RNP particles also restrict access of the translational machinery and micro-RNAs to the transcript and in this way affect rates of protein translation (reviewed in Bell et al, 2013). IGFBP1 binds to the 3' UTR of MAPK4 mRNA and inhibits its translation. This antagonizes MAPKAPK5 activation and HSBP1 phosphorylation and in this manner affects F-actin rearrangements and cell motility (Stohr et al, 2012; Kostenko et al, 2009a; reviewed in Kostenko et al, 2012).Authored: Rothfels, Karen, 2015-03-30Reviewed: Moens, Ugo, 2015-04-24Reviewed: Seternes, Ole-Morten, 2015-05-05Reviewed: Mathien, Simon, 2015-05-12Reviewed: Meloche, Sylvain, 2015-05-12Reviewed: Soulez, Mathilde, 2015-05-12Edited: Rothfels, Karen, 2015-05-13Reactome DB_ID: 56869761cytosolGO0005829ENSEMBL:ENST00000400384 MAPK4MAPK4 mRNAMAPK4Reactomehttp://www.reactome.orgHomo sapiensNCBI Taxonomy9606ENSEMBLENST00000400384Chain Coordinates1EQUAL4770EQUALReactome DB_ID: 4283661UniProt:Q9NZI8 IGF2BP1IGF2BP1ZBP1CRDBPVICKZ1IGF2BP1FUNCTION RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Plays a direct role in the transport and translation of transcripts required for axonal regeneration in adult sensory neurons (By similarity). Regulates localized beta-actin/ACTB mRNA translation, a crucial process for cell polarity, cell migration and neurite outgrowth. Co-transcriptionally associates with the ACTB mRNA in the nucleus. This binding involves a conserved 54-nucleotide element in the ACTB mRNA 3'-UTR, known as the 'zipcode'. The RNP thus formed is exported to the cytoplasm, binds to a motor protein and is transported along the cytoskeleton to the cell periphery. During transport, prevents ACTB mRNA from being translated into protein. When the RNP complex reaches its destination near the plasma membrane, IGF2BP1 is phosphorylated. This releases the mRNA, allowing ribosomal 40S and 60S subunits to assemble and initiate ACTB protein synthesis. Monomeric ACTB then assembles into the subcortical actin cytoskeleton (By similarity). During neuronal development, key regulator of neurite outgrowth, growth cone guidance and neuronal cell migration, presumably through the spatiotemporal fine tuning of protein synthesis, such as that of ACTB (By similarity). May regulate mRNA transport to activated synapses (By similarity). Binds to and stabilizes ABCB1/MDR-1 mRNA (By similarity). During interstinal wound repair, interacts with and stabilizes PTGS2 transcript. PTGS2 mRNA stabilization may be crucial for colonic mucosal wound healing (By similarity). Binds to the 3'-UTR of IGF2 mRNA by a mechanism of cooperative and sequential dimerization and regulates IGF2 mRNA subcellular localization and translation. Binds to MYC mRNA, in the coding region instability determinant (CRD) of the open reading frame (ORF), hence prevents MYC cleavage by endonucleases and possibly microRNA targeting to MYC-CRD. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to the oncofetal H19 transcript and to the neuron-specific TAU mRNA and regulates their localizations. Binds to and stabilizes BTRC/FBW1A mRNA. Binds to the adenine-rich autoregulatory sequence (ARS) located in PABPC1 mRNA and represses its translation. PABPC1 mRNA-binding is stimulated by PABPC1 protein. Prevents BTRC/FBW1A mRNA degradation by disrupting microRNA-dependent interaction with AGO2. Promotes the directed movement of tumor-derived cells by fine-tuning intracellular signaling networks. Binds to MAPK4 3'-UTR and inhibits its translation. Interacts with PTEN transcript open reading frame (ORF) and prevents mRNA decay. This combined action on MAPK4 (down-regulation) and PTEN (up-regulation) antagonizes HSPB1 phosphorylation, consequently it prevents G-actin sequestration by phosphorylated HSPB1, allowing F-actin polymerization. Hence enhances the velocity of cell migration and stimulates directed cell migration by PTEN-modulated polarization. Interacts with Hepatitis C virus (HCV) 5'-UTR and 3'-UTR and specifically enhances translation at the HCV IRES, but not 5'-cap-dependent translation, possibly by recruiting eIF3. Interacts with HIV-1 GAG protein and blocks the formation of infectious HIV-1 particles. Reduces HIV-1 assembly by inhibiting viral RNA packaging, as well as assembly and processing of GAG protein on cellular membranes. During cellular stress, such as oxidative stress or heat shock, stabilizes target mRNAs that are recruited to stress granules, including CD44, IGF2, MAPK4, MYC, PTEN, RAPGEF2 and RPS6KA5 transcripts.SUBUNIT Can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. During HCV infection, identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Interacts (via the KH domains) with HIV-1 GAG (via the second zinc finger motif of NC). Associates (via the RRM domains and KH domains) with HIV-1 particles. Identified in a mRNP complex, composed of at least DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with DHX9, ELAVL2, HNRNPA2B1, HNRNPC, HNRNPH1, HNRNPU, IGF2BP2, ILF2, and YBX1. Interacts with FMR1. Component of a multisubunit autoregulatory RNP complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1/UNR. Directly interacts with PABPC1. Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP. Interacts with ELAVL4 in an RNA-dependent manner. Associates with microtubules and polysomes. Interacts with AGO1 and AGO2.TISSUE SPECIFICITY Mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus (at protein level). Also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta (at protein level). Expressed in various cancers, including testis and lung cancers (at protein level), as well as kidney, prostate and trachea cancers.INDUCTION May be up-regulated in response to CTNNB1/beta-catenin activation.DOMAIN Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for RNA-dependent homo- and heterooligomerization and for localization to stress granules. KH3 and KH4 mediate association with the cytoskeleton. Two nuclear export signals (NES) have been identified in KH2 and KH4 domains, respectively. Only KH2 NES is XPO1-dependent. Both NES may be redundant, since individual in vitro mutations do not affect subcellular location of the full-length protein. The 4 KH domains are important to suppress HIV-1 infectivity.PTM Phosphorylated. Phosphorylation may impair association with ACTB mRNA and hence abolishes translational repression (By similarity).SIMILARITY Belongs to the RRM IMP/VICKZ family.UniProtQ9NZI81EQUAL577EQUALReactome DB_ID: 56870701IGF2BP1:MAPK4 mRNA [cytosol]IGF2BP1:MAPK4 mRNAReactome DB_ID: 568697611EQUAL4770EQUALReactome DB_ID: 42836611EQUAL577EQUALReactome Database ID Release 765687070Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=5687070ReactomeR-HSA-56870701Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-5687070.1Reactome Database ID Release 765687079Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=5687079ReactomeR-HSA-56870791Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-5687079.122279049Pubmed2012IGF2BP1 promotes cell migration by regulating MK5 and PTEN signalingStöhr, NadineKöhn, MarcelLederer, MarcellGlass, MarkusReinke, ClaudiaSinger, Robert HHüttelmaier, StefanGenes Dev. 26:176-8922800433Pubmed2012Tumour promoting and suppressing roles of the atypical MAP kinase signalling pathway ERK3/4-MK5Kostenko, SergiyDumitriu, GianinaMoens, UgoJ Mol Signal 7:919166925Pubmed2009PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5Kostenko, SergiyJohannessen, MonaMoens, UgoCell. Signal. 21:712-823069990Pubmed2013Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs): post-transcriptional drivers of cancer progression?Bell, Jessica LWächter, KristinMühleck, BrittaPazaitis, NikolaosKöhn, MarcelLederer, MarcellHüttelmaier, StefanCell. Mol. Life Sci. 70:2657-75