BioPAX pathway converted from "E2F1 binds RAD51 gene promoter" in the Reactome database.E2F1 binds RAD51 gene promoterE2F1 binds RAD51 gene promoterE2F1, presumably in complex with TFDP1 (DP-1) or TFDP2 (DP-2) binds the promoter of the RAD51 gene (Bertoli et al. 2013, Wu et al. 2014).Authored: Orlic-Milacic, Marija, 2017-05-31Reviewed: Herlihy, Anna, 2017-05-12Edited: Orlic-Milacic, Marija, 2017-05-31Reactome DB_ID: 90075451nucleoplasmGO0005654ENSEMBL:ENSG00000051180 RAD51RAD51ARAD51RECAReactomehttp://www.reactome.orgHomo sapiensNCBI Taxonomy9606ENSEMBLENSG00000051180Reactome DB_ID: 90075121E2F1:(TFDP1,TFDP2) [nucleoplasm]E2F1:(TFDP1,TFDP2)Reactome DB_ID: 686391UniProt:Q01094 E2F1E2F1RBBP3E2F1FUNCTION Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Positively regulates transcription of RRP1B (PubMed:20040599).ACTIVITY REGULATION BIRC2/c-IAP1 stimulates its transcriptional activity.SUBUNIT Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB1, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity. Interacts with BIRC2/c-IAP1 (via BIR domains). The complex TFDP1:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target genes promoters and represses its transcriptional activity (PubMed:20176812). Interacts with RRP1B (PubMed:20040599). Interacts with HCFC1 (PubMed:23629655). Interacts with KMT2E; the interaction is probably indirect and is mediated via HCFC1 (PubMed:23629655).SUBUNIT (Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL123.PTM Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis.PTM Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.SIMILARITY Belongs to the E2F/DP family.UniProtQ01094Chain Coordinates1EQUAL437EQUALConverted from EntitySet in ReactomeReactome DB_ID: 90075081(TFDP1,TFDP2) [nucleoplasm]Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntityReactome Database ID Release 719007512Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9007512ReactomeR-HSA-90075121Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-9007512.1Reactome DB_ID: 90075721E2F1:(TFDP1,TFDP2):RAD51 gene [nucleoplasm]E2F1:(TFDP1,TFDP2):RAD51 geneReactome DB_ID: 90075451Reactome DB_ID: 90075121Reactome Database ID Release 719007572Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9007572ReactomeR-HSA-90075721Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-9007572.1Reactome Database ID Release 719007561Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9007561ReactomeR-HSA-90075611Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-9007561.123954429Pubmed2013Chk1 inhibits E2F6 repressor function in response to replication stress to maintain cell-cycle transcriptionBertoli, CosettaKlier, SteffiMcGowan, ClareWittenberg, Curtde Bruin, Robertus A MCurr. Biol. 23:1629-3724627085Pubmed2014Dynamic regulation of Rad51 by E2F1 and p53 in prostate cancer cells upon drug-induced DNA damage under hypoxiaWu, MinghuiWang, XueMcGregor, NataliePienta, Kenneth JZhang, JingsongMol. Pharmacol. 85:866-76