BioPAX pathway converted from "pp1a cleaves itself" in the Reactome database. 3.4.22.41 3.4.22.40 3.4.22.34 3.4.22.8 3.4.22.28 3.4.22.16 3.4.22.27 3.4.22.38 3.4.22.1 3.4.22.15 3.4.22.14 pp1a cleaves itself pp1a cleaves itself The crucial step of autocleavage of pp1a involves the formation of an "intermediate" pp1a dimer which has weak protease activity. This "embedded" 3CLp liberates itself by cleaving the ends off its monomer in trans. Only after that the cleaved 3CLp forms a dimer, the most efficient form of the enzyme (Hsu et al, 2005; Chen et al, 2010; Muramatsu et al, 2016). Authored: Stephan, Ralf, 2020-04-14 Reviewed: Acencio, Marcio Luis, Mazein, Alexander, 2020-05-27 Edited: D'Eustachio, Peter, 2020-05-21 Reactome DB_ID: 9684357 1 cytosol GO 0005829 UniProt:P0C6U8 1a 1a 1a SIMILARITY Belongs to the coronaviruses polyprotein 1ab family.CAUTION Isolates SZ3 and SZ16 have been isolated from Paguma larvata and are described as SARS-like in literature. Reactome http://www.reactome.org Human SARS coronavirus NCBI Taxonomy 694009 UniProt P0C6U8 Reactome DB_ID: 29356 2 water [ChEBI:15377] water ChEBI 15377 Reactome DB_ID: 9684354 1 Chain Coordinates 3547 EQUAL 4382 EQUAL Reactome DB_ID: 9678259 1 3241 EQUAL 3546 EQUAL Reactome DB_ID: 9684311 1 1 EQUAL 3240 EQUAL PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 9684326 pp1a dimer [cytosol] pp1a dimer Reactome DB_ID: 9684357 2 Reactome Database ID Release 81 9684326 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684326 Reactome R-COV-9684326 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-COV-9684326.1 GO 0004197 GO molecular function Reactome Database ID Release 81 9684283 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684283 Reactome Database ID Release 81 9684351 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684351 Reactome R-HSA-9684351 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-9684351.2 21203998 Pubmed 2010 Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode Chen, Shuai Jonas, Felix Shen, Can Hilgenfeld, Rolf Higenfeld, Rolf Protein Cell 1:59-74 15788388 Pubmed 2005 Mechanism of the maturation process of SARS-CoV 3CL protease Hsu, Min-Feng Kuo, Chih-Jung Chang, Kai-Ti Chang, Hui-Chuan Chou, Chia-Cheng Ko, Tzu-Ping Shr, Hui-Lin Chang, Gu-Gang Wang, Andrew H-J Liang, Po-Huang J. Biol. Chem. 280:31257-66 27799534 Pubmed 2016 SARS-CoV 3CL protease cleaves its C-terminal autoprocessing site by novel subsite cooperativity Muramatsu, Tomonari Takemoto, Chie Kim, Yong-Tae Wang, Hongfei Nishii, Wataru Terada, Takaho Shirouzu, Mikako Yokoyama, Shigeyuki Proc. Natl. Acad. Sci. U.S.A. 113:12997-13002