BioPAX pathway converted from "Reactions specific to the hybrid N-glycan synthesis pathway" in the Reactome database.Reactions specific to the hybrid N-glycan synthesis pathwayThis event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>LEFT-TO-RIGHT2.4.1.144Addition of a bifurcating GlcNAc to the N-glycan by MGAT3This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>NGPN-glycoproteinN-glycanReactome DB_ID: 975910Golgi lumenGENE ONTOLOGYGO:0005796N-glycan [ChEBI:59520]N-glycanChEBICHEBI:59520Reactome Database ID Release 75975910Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=975910ReactomeR-ALL-9759103Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-975910.3Reactomehttp://www.reactome.orgUDP-GlcNAcUDP-N-acetyl-D-glucosamineUDP-N-acetyl-alpha-D-glucosamineUDP-N-acetylglucosamineReactome DB_ID: 914003UDP-N-acetyl-alpha-D-glucosamine [ChEBI:16264]UDP-N-acetyl-alpha-D-glucosamineChEBICHEBI:16264Reactome Database ID Release 75914003Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=914003ReactomeR-ALL-9140033Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-914003.3Glycoprotein with bifurcating GlcNAc in position 3Reactome DB_ID: 981613Reactome Database ID Release 75981613Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=981613ReactomeR-ALL-9816132Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-981613.2ChEBI33837additional informationMIMI:0361ACTIVATIONMGAT3mgat3bA0A0G2KRY8Reactome DB_ID: 10233586Golgi membraneGENE ONTOLOGYGO:0000139UniProt:A0A0G2KRY8mgat3bDanio rerioNCBI Taxonomy7955UniProtA0A0G2KRY81EQUAL533EQUALReactome Database ID Release 7510233586Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10233586ReactomeR-DRE-9758971Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-975897.1GENE ONTOLOGYGO:0003830gene ontology term for cellular functionMIMI:0355Same Catalyst ActivityReactome Database ID Release 7510233587Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10233587Reactome Database ID Release 7510233589Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10233589ReactomeR-DRE-9759261Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-975926.1The addition of a bisecting GlcNAc to a complex N-glycan by MGAT3 is one of the most important regulatory steps in N-glycosylation, directing the pathway toward the synthesis of complex and hybrid N-glycans. This addition changes the structure of the N-glycan and inhibits further modification by MGAT2, MGAT4, MGAT5A/B and FUT8. Defects in MGAT3 have been shown to be associated with predisposition to cancer and several developmental defects (Song et al 2010; Stanley 2002).20395209Pubmed2010The bisecting GlcNAc on N-glycans inhibits growth factor signaling and retards mammary tumor progressionSong, YAglipay, JABernstein, JDGoswami, SStanley, PCancer Res 70:3361-7112417419Pubmed2002Biological consequences of overexpressing or eliminating N-acetylglucosaminyltransferase-TIII in the mouseStanley, PBiochim Biophys Acta 1573:363-8inferred from electronic annotationEVIDENCE CODEECO:0000203Reactome Database ID Release 7510304895Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=10304895ReactomeR-DRE-9755741Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-DRE-975574.1The transfer of a bisecting GlcNAc by MGAT3 commits the pathway toward the synthesis of hybrid glycans, because MAN2 is not able to operate on bisected oligosaccharides (Schachter et al 2000, Priatel JJ et al, 1997). The expression of MGAT3 over MGAT2 in a tissue can regulate the synthesis of hybrid toward complex N-glycans. The addition of a GlcNAc between the two arms also prevents the action of MGAT4, MGAT5 and FUT8.9061364Pubmed1997Isolation, characterization and inactivation of the mouse Mgat3 gene: the bisecting N-acetylglucosamine in asparagine-linked oligosaccharides appears dispensable for viability and reproductionPriatel, JJSarkar, MSchachter, HMarth, JDGlycobiology 7:45-5611421343Pubmed2000The joys of HexNAc. The synthesis and function of N- and O-glycan branchesSchachter, HGlycoconj J 17:465-83