BioPAX pathway converted from "Exocytosis of platelet dense granule content" in the Reactome database. LEFT-TO-RIGHT Exocytosis of platelet dense granule content ADP is the most important constituent of dense granules, essential for recruiting platelets to the site of vascular injury. Other components include ATP, Ca++, GDP, GTP, Mg++, orthophospate, pyrophosphate, and serotonin. Authored: de Bono, B, Pace, N.P., Farndale, R, 2004-09-25 17:06:19 Converted from EntitySet in Reactome Platelet dense granule content Reactome DB_ID: 481002 ADP Adenosine 5'-diphosphate ADP(3-) Reactome DB_ID: 114565 platelet dense granule lumen GENE ONTOLOGY GO:0031089 ADP(3-) [ChEBI:456216] ADP(3-) ADP 5'-O-[(phosphonatooxy)phosphinato]adenosine ADP trianion ChEBI CHEBI:456216 Reactome Database ID Release 82 114565 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114565 Reactome R-ALL-114565 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114565.3 Reactome http://www.reactome.org COMPOUND C00008 additional information MI MI:0361 ATP Adenosine 5'-triphosphate ATP(4-) Reactome DB_ID: 114571 ATP(4-) [ChEBI:30616] ATP(4-) ATP atp Adenosine 5'-triphosphate ChEBI CHEBI:30616 Reactome Database ID Release 82 114571 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114571 Reactome R-ALL-114571 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114571.3 COMPOUND C00002 Ca2+ Calcium calcium(2+) Reactome DB_ID: 114582 calcium(2+) [ChEBI:29108] calcium(2+) ChEBI CHEBI:29108 Reactome Database ID Release 82 114582 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114582 Reactome R-ALL-114582 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114582.3 COMPOUND C00076 GDP Guanosine 5'-diphosphate Guanosine diphosphate Reactome DB_ID: 114622 GDP [ChEBI:17552] GDP Guanosine 5'-diphosphate Guanosine diphosphate ChEBI CHEBI:17552 Reactome Database ID Release 82 114622 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114622 Reactome R-ALL-114622 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114622.2 COMPOUND C00035 GTP Guanosine 5'-triphosphate Reactome DB_ID: 114625 GTP [ChEBI:15996] GTP Guanosine 5'-triphosphate ChEBI CHEBI:15996 Reactome Database ID Release 82 114625 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114625 Reactome R-ALL-114625 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114625.2 COMPOUND C00044 Mg2+ Magnesium magnesium(2+) Mg++ Reactome DB_ID: 114631 magnesium(2+) [ChEBI:18420] magnesium(2+) ChEBI CHEBI:18420 Reactome Database ID Release 82 114631 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114631 Reactome R-ALL-114631 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114631.3 COMPOUND C00305 Pi Orthophosphate hydrogenphosphate inorganic phosphate phosphate Reactome DB_ID: 114640 hydrogenphosphate [ChEBI:43474] hydrogenphosphate hydrogen phosphate phosphate [PO3(OH)](2-) INORGANIC PHOSPHATE GROUP HYDROGENPHOSPHATE ION HPO4(2-) [P(OH)O3](2-) ChEBI CHEBI:43474 Reactome Database ID Release 82 114640 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114640 Reactome R-ALL-114640 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114640.4 COMPOUND C00009 PPi pyrophosphate diphosphoric acid pyrophosphoric acid diphosphate Reactome DB_ID: 114654 diphosphate(3-) [ChEBI:33019] diphosphate(3-) ChEBI CHEBI:33019 Reactome Database ID Release 82 114654 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114654 Reactome R-ALL-114654 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114654.4 COMPOUND C00013 5HT 5-Hydroxytryptamine serotonin 3-(2-Aminoethyl)-1H-indol-5-ol Enteramine Reactome DB_ID: 30717 serotonin(1+) [ChEBI:350546] serotonin(1+) serotonin serotonin cation 2-(5-hydroxy-1H-indol-3-yl)ethanaminium ChEBI CHEBI:350546 Reactome Database ID Release 82 30717 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=30717 Reactome R-ALL-30717 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-30717.4 COMPOUND C00780 TIMP3 Metalloproteinase inhibitor 3 TIMP3_HUMAN Reactome DB_ID: 8848904 UniProt:P35625 TIMP3 TIMP3 FUNCTION Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.SUBUNIT Interacts with EFEMP1.SIMILARITY Belongs to the protease inhibitor I35 (TIMP) family. Homo sapiens NCBI Taxonomy 9606 UniProt P35625 24 EQUAL 211 EQUAL Reactome Database ID Release 82 8848904 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848904 Reactome R-HSA-8848904 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848904.1 LGALS3BP Galectin-3-binding protein LG3BP_HUMAN Reactome DB_ID: 8848916 UniProt:Q08380 LGALS3BP LGALS3BP M2BP FUNCTION Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.SUBUNIT Homodimers and homomultimers. The multimers form ring-like structures with a diameter of 30-40 nm. Binds LGALS1 and LGALS3. Binds ITGB1, COL4A1, COL5A1, COL6A1, FN1 and NID. Interacts with the gamma-tubulin ring complex (gamma-TuRC), composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 (PubMed:29162697). The unglycosylated form interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction may connect a pericentrosomal complex, made of AKAP9, CDK5RAP2, EB1/MAPRE1 and PDE4DIP, to the gamma-tubulin ring complex (gamma-TuRC) to promote microtubule assembly and acetylation (PubMed:29162697).TISSUE SPECIFICITY Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. UniProt Q08380 19 EQUAL 585 EQUAL Reactome Database ID Release 82 8848916 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848916 Reactome R-HSA-8848916 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848916.1 RARRES2 Retinoic acid receptor responder protein 2 RARR2_HUMAN Reactome DB_ID: 8848881 UniProt:Q99969 RARRES2 RARRES2 TIG2 FUNCTION Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Acts also as a ligand for CMKLR2. Can also bind to C-C chemokine receptor-like 2 (CCRL2), but with a lower affinity than it does to CMKLR1 or CMKLR2 (PubMed:27716822). Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a pro-inflammatory adipokine, causing an increase in secretion of pro-inflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Its dual role in inflammation and metabolism might provide a link between chronic inflammation and obesity, as well as obesity-related disorders such as type 2 diabetes and cardiovascular disease. Exhibits an antimicrobial function in the skin.TISSUE SPECIFICITY Expressed at the highest levels in placenta, liver, and white adipose tissue (WAT), and to a lesser extent in many other tissues such as lung, brown adipose tissue, heart, ovary, kidney, skeletal muscle and pancreas. Within WAT, expression is enriched in adipocytes as compared to the stromal vascular fraction. Expression and secretion increases dramatically with adipogenesis. Highly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Expression is elevated in numerous metabolic and inflammatory diseases including psoriasis, obesity, type 2 diabetes, metabolic syndrome and cardiovascular disease.INDUCTION Inhibited in psoriatic lesions. Activated by tazarotene in skin rafts and in the epidermis of psoriatic lesions.PTM Secreted in an inactive precursor form, prochemerin, which is proteolytically processed by a variety of extracellular proteases to generate forms with differing levels of bioactivity. For example, the removal of six amino acids results in chemerin-157, which exhibits the highest activity, while removal of seven amino acids results in chemerin-156 which has slightly less activity. Some proteases are able to cleave at more than one site and chemerin forms may be sequentially processed by different enzymes to modulate activity levels. The coordinated expression and activity of chemerin-modifying enzymes is essential for regulating its bioactivation, inactivation and, consequently, biological function. Cathepsin G cleaves seven C-terminal amino acids from prochemerin (chemerin-156), elastase is able to cleave six (chemerin-157), eight (chemerin-155) or eleven (chemerin-152), plasmin cleaves five amino acids (chemerin-158), and tryptase cleaves five (chemerin-158) or eight (chemerin-155). Multiple cleavages might be required to fully activate chemerin, with an initial tryptase cleavage resulting in chemerin with low activity (chemerin-158), and a second cleavage by carboxypeptidase N or B producing highly active chemerin (chemerin-157). UniProt Q99969 21 EQUAL 157 EQUAL Reactome Database ID Release 82 8848881 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848881 Reactome R-HSA-8848881 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848881.1 SEPP1 Selenoprotein P SEPP1_HUMAN Reactome DB_ID: 8848897 UniProt:P49908 SELENOP SELENOP SELP SEPP1 FUNCTION Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.TISSUE SPECIFICITY Made in the liver and heart and secreted into the plasma. It is also found in the kidney.DOMAIN The C-terminus is not required for endocytic uptake in the proximal tubule epithelium.PTM Phosphorylation sites are present in the extracellular medium.SIMILARITY Belongs to the selenoprotein P family. UniProt P49908 20 EQUAL 381 EQUAL Reactome Database ID Release 82 8848897 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848897 Reactome R-HSA-8848897 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848897.1 SERPINA4 Kallistatin KAIN_HUMAN Reactome DB_ID: 8848911 UniProt:P29622 SERPINA4 SERPINA4 KST PI4 FUNCTION Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.SUBUNIT Monomer and some homodimers.TISSUE SPECIFICITY Expressed by the liver and secreted in plasma.PTM The N-terminus is blocked.MISCELLANEOUS Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.SIMILARITY Belongs to the serpin family. UniProt P29622 21 EQUAL 427 EQUAL Reactome Database ID Release 82 8848911 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848911 Reactome R-HSA-8848911 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848911.1 SPP2 Secreted phosphoprotein 24 SPP24_HUMAN Reactome DB_ID: 8848900 UniProt:Q13103 SPP2 SPP2 SPP24 FUNCTION Could coordinate an aspect of bone turnover.TISSUE SPECIFICITY Detected in liver and plasma.DEVELOPMENTAL STAGE Found in fetal liver and kidney.PTM Phosphorylation sites are present in the extracellular medium.SIMILARITY Belongs to the SPP2 family. UniProt Q13103 30 EQUAL 211 EQUAL Reactome Database ID Release 82 8848900 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848900 Reactome R-HSA-8848900 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848900.1 ITIH3 Inter-alpha-trypsin inhibitor heavy chain H3 ITIH3_HUMAN Reactome DB_ID: 8848908 UniProt:Q06033 ITIH3 ITIH3 FUNCTION May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.SUBUNIT I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (HC) and one light chain, bikunin. Pre-alpha-inhibitor (P-alpha-I) is composed of ITIH3/HC3 and bikunin.PTM Heavy chains are linked to bikunin via chondroitin 4-sulfate esterified to the alpha-carboxyl of the C-terminal aspartate after propeptide cleavage.SIMILARITY Belongs to the ITIH family. UniProt Q06033 35 EQUAL 651 EQUAL Reactome Database ID Release 82 8848908 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848908 Reactome R-HSA-8848908 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848908.1 ITIH4 Inter-alpha-trypsin inhibitor heavy chain H4 ITIH4_HUMAN Reactome DB_ID: 8848895 UniProt:Q14624 ITIH4 ITIH4 IHRP ITIHL1 PK120 PRO1851 FUNCTION Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.SUBUNIT Interacts (via C-terminus) with DNAJC1 (via SANT 2 domain); this interaction protects ITIH4 against cleavage by kallikrein in vitro.TISSUE SPECIFICITY Liver specific.INDUCTION Levels increase from 1.4 to 3-fold in acute-phase processes such as in acute ischemia stroke (AIS), unstable angina and programmed surgery. In hepatocytes, induced by IL6 but not by other cytokines such as IL1B.PTM Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.PTM N- and O-glycosylated. In urine, O-linked glycosylation on threonine residues in the region from Thr-719 to Thr-725 consists of core 1 or possibly core 8 glycans. Mainly Hex(HexNAc)(2), but also some Hex(3)(HexNAc)(3). N-glycosylated but not O-glycosylated in plasma.MISCELLANEOUS Possible biomarker for acute ischemic stroke (PubMed:19263524). Peptides derived from the proline-rich potentially active peptide (PRO_0000016542) may be biomarkers for a variety of disease states including breast cancer (PubMed:21137033).SIMILARITY Belongs to the ITIH family. UniProt Q14624 29 EQUAL 661 EQUAL Reactome Database ID Release 82 8848895 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848895 Reactome R-HSA-8848895 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848895.1 APOH Beta-2-glycoprotein 1 APOH_HUMAN Reactome DB_ID: 8848901 UniProt:P02749 APOH APOH B2G1 FUNCTION Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.TISSUE SPECIFICITY Expressed by the liver and secreted in plasma.PTM N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. UniProt P02749 20 EQUAL 345 EQUAL Reactome Database ID Release 82 8848901 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848901 Reactome R-HSA-8848901 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848901.1 CDC37L1 Hsp90 co-chaperone Cdc37-like 1 CD37L_HUMAN Reactome DB_ID: 8848903 UniProt:Q7L3B6 CDC37L1 CDC37L1 CDC37B HARC FUNCTION Co-chaperone that binds to numerous proteins and promotes their interaction with Hsp70 and Hsp90.SUBUNIT Self-associates. Forms complexes with Hsp70 and Hsp90. Interacts with CDC37, FKBP4, PPID and STIP1.TISSUE SPECIFICITY Expressed in brain, heart, kidney, liver, placenta and skeletal muscle.SIMILARITY Belongs to the CDC37 family. UniProt Q7L3B6 1 EQUAL 337 EQUAL Reactome Database ID Release 82 8848903 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848903 Reactome R-HSA-8848903 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848903.1 CLEC3B Tetranectin TETN_HUMAN Reactome DB_ID: 8848923 UniProt:P05452 CLEC3B CLEC3B TNA FUNCTION Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.SUBUNIT Homotrimer.TISSUE SPECIFICITY Found in plasma. UniProt P05452 22 EQUAL 202 EQUAL Reactome Database ID Release 82 8848923 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848923 Reactome R-HSA-8848923 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848923.1 CTSW Cathepsin W ecNumber3.4.22.-/ecNumber CATW_HUMAN Reactome DB_ID: 8848924 UniProt:P56202 CTSW CTSW FUNCTION May have a specific function in the mechanism or regulation of T-cell cytolytic activity.FUNCTION (Microbial infection) Plays a role during influenza virus infection in lungs cells ex vivo. Acts at the level of virus entering host cytoplasm from late endosome.TISSUE SPECIFICITY Expressed predominantly in natural killer cells, and in cytotoxic T cells.SIMILARITY Belongs to the peptidase C1 family. UniProt P56202 128 EQUAL 376 EQUAL Reactome Database ID Release 82 8848924 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848924 Reactome R-HSA-8848924 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848924.1 ECM1 Extracellular matrix protein 1 ECM1_HUMAN Reactome DB_ID: 8848880 UniProt:Q16610 ECM1 ECM1 FUNCTION Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.SUBUNIT Interacts (via C-terminus) with HSPG2 (via C-terminus). Interacts with EFEMP1/FBLN3 and LAMB3. Interacts with MMP9.TISSUE SPECIFICITY Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. UniProt Q16610 20 EQUAL 540 EQUAL Reactome Database ID Release 82 8848880 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848880 Reactome R-HSA-8848880 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848880.1 FAM3C Protein FAM3C FAM3C_HUMAN Reactome DB_ID: 8848884 UniProt:Q92520 FAM3C FAM3C ILEI GS3786 FUNCTION May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.TISSUE SPECIFICITY Present in most secretory epithelia (at protein level).MISCELLANEOUS Up-regulation and/or mislocalization in breast cancer and liver carcinoma cells is strongly correlated with metastasis formation and survival.SIMILARITY Belongs to the FAM3 family. UniProt Q92520 25 EQUAL 227 EQUAL Reactome Database ID Release 82 8848884 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848884 Reactome R-HSA-8848884 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848884.1 Reactome Database ID Release 82 481002 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=481002 Reactome R-HSA-481002 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-481002.3 Converted from EntitySet in Reactome Platelet dense granule content Reactome DB_ID: 481030 ADP Adenosine 5'-diphosphate ADP(3-) Reactome DB_ID: 114564 extracellular region GENE ONTOLOGY GO:0005576 Reactome Database ID Release 82 114564 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114564 Reactome R-ALL-114564 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114564.4 COMPOUND C00008 ATP Adenosine 5'-triphosphate ATP(4-) Reactome DB_ID: 114570 Reactome Database ID Release 82 114570 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114570 Reactome R-ALL-114570 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114570.3 COMPOUND C00002 Ca2+ Calcium calcium(2+) Reactome DB_ID: 74112 Reactome Database ID Release 82 74112 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=74112 Reactome R-ALL-74112 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-74112.4 COMPOUND C00076 GDP Guanosine 5'-diphosphate Guanosine diphosphate Reactome DB_ID: 114623 GDP(3-) [ChEBI:58189] GDP(3-) 5'-O-[(phosphonatooxy)phosphinato]guanosine guanosine 5'-diphosphate(3-) GDP guanosine 5'-diphosphate trianion guanosine 5'-diphosphate GDP trianion ChEBI CHEBI:58189 Reactome Database ID Release 82 114623 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114623 Reactome R-ALL-114623 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114623.3 COMPOUND C00035 GTP Guanosine 5'-triphosphate Reactome DB_ID: 114626 GTP(4-) [ChEBI:37565] GTP(4-) GTP gtp guanosine 5'-triphosphate(4-) ChEBI CHEBI:37565 Reactome Database ID Release 82 114626 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114626 Reactome R-ALL-114626 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114626.3 COMPOUND C00044 Mg2+ Magnesium magnesium(2+) Mg++ Reactome DB_ID: 114632 Reactome Database ID Release 82 114632 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114632 Reactome R-ALL-114632 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114632.4 COMPOUND C00305 Pi Orthophosphate hydrogenphosphate inorganic phosphate phosphate Reactome DB_ID: 109277 Reactome Database ID Release 82 109277 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=109277 Reactome R-ALL-109277 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-109277.4 COMPOUND C00009 PPi pyrophosphate diphosphoric acid pyrophosphoric acid diphosphate Reactome DB_ID: 113542 Reactome Database ID Release 82 113542 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=113542 Reactome R-ALL-113542 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-113542.4 COMPOUND C00013 5HT 5-Hydroxytryptamine serotonin 3-(2-Aminoethyl)-1H-indol-5-ol Enteramine Reactome DB_ID: 114523 Reactome Database ID Release 82 114523 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=114523 Reactome R-ALL-114523 4 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-114523.4 COMPOUND C00780 SEPP1 Selenoprotein P SEPP1_HUMAN Reactome DB_ID: 8848887 20 EQUAL 381 EQUAL Reactome Database ID Release 82 8848887 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848887 Reactome R-HSA-8848887 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848887.1 SERPINA4 Kallistatin KAIN_HUMAN Reactome DB_ID: 8848886 21 EQUAL 427 EQUAL Reactome Database ID Release 82 8848886 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848886 Reactome R-HSA-8848886 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848886.1 SPP2 Secreted phosphoprotein 24 SPP24_HUMAN Reactome DB_ID: 8848914 30 EQUAL 211 EQUAL Reactome Database ID Release 82 8848914 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848914 Reactome R-HSA-8848914 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848914.1 TIMP3 Metalloproteinase inhibitor 3 TIMP3_HUMAN Reactome DB_ID: 1430774 24 EQUAL 211 EQUAL Reactome Database ID Release 82 1430774 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1430774 Reactome R-HSA-1430774 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-1430774.1 APOH Beta-2-glycoprotein 1 APOH_HUMAN Reactome DB_ID: 8848905 20 EQUAL 345 EQUAL Reactome Database ID Release 82 8848905 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848905 Reactome R-HSA-8848905 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848905.1 CDC37L1 Hsp90 co-chaperone Cdc37-like 1 CD37L_HUMAN Reactome DB_ID: 8848918 1 EQUAL 337 EQUAL Reactome Database ID Release 82 8848918 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848918 Reactome R-HSA-8848918 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848918.1 CLEC3B Tetranectin TETN_HUMAN Reactome DB_ID: 8848885 22 EQUAL 202 EQUAL Reactome Database ID Release 82 8848885 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848885 Reactome R-HSA-8848885 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848885.1 CTSW Cathepsin W ecNumber3.4.22.-/ecNumber CATW_HUMAN Reactome DB_ID: 8848921 128 EQUAL 376 EQUAL Reactome Database ID Release 82 8848921 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848921 Reactome R-HSA-8848921 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848921.1 ECM1 Extracellular matrix protein 1 ECM1_HUMAN Reactome DB_ID: 8848909 20 EQUAL 540 EQUAL Reactome Database ID Release 82 8848909 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848909 Reactome R-HSA-8848909 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848909.1 FAM3C Protein FAM3C FAM3C_HUMAN Reactome DB_ID: 8848922 25 EQUAL 227 EQUAL Reactome Database ID Release 82 8848922 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848922 Reactome R-HSA-8848922 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848922.1 ITIH3 Inter-alpha-trypsin inhibitor heavy chain H3 ITIH3_HUMAN Reactome DB_ID: 8848920 35 EQUAL 651 EQUAL Reactome Database ID Release 82 8848920 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848920 Reactome R-HSA-8848920 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848920.1 ITIH4 Inter-alpha-trypsin inhibitor heavy chain H4 ITIH4_HUMAN Reactome DB_ID: 8848892 29 EQUAL 661 EQUAL Reactome Database ID Release 82 8848892 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848892 Reactome R-HSA-8848892 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848892.1 LGALS3BP Galectin-3-binding protein LG3BP_HUMAN Reactome DB_ID: 8848882 19 EQUAL 585 EQUAL Reactome Database ID Release 82 8848882 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848882 Reactome R-HSA-8848882 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848882.1 RARRES2 Retinoic acid receptor responder protein 2 RARR2_HUMAN Reactome DB_ID: 8848915 21 EQUAL 157 EQUAL Reactome Database ID Release 82 8848915 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=8848915 Reactome R-HSA-8848915 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8848915.1 Reactome Database ID Release 82 481030 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=481030 Reactome R-HSA-481030 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-481030.3 Reactome Database ID Release 82 481009 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=481009 Reactome R-HSA-481009 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-481009.3 19073150 Pubmed 2009 RhoA downstream of G(q) and G(12/13) pathways regulates protease-activated receptor-mediated dense granule release in platelets Jin, J Mao, Y Thomas, D Kim, S Daniel, JL Kunapuli, SP Biochem Pharmacol 77:835-44 1435334 Pubmed 1992 Isolation of dense granules from human platelets Fukami, MH Methods Enzymol 215:36-42 4132863 Pubmed 1974 Calcium-containing platelet granules Martin, JH Carson, FL Race, GJ J Cell Biol 60:775-7 4809565 Pubmed 1974 Serotonin storage in platelets: estimation of storage-packet size Costa, JL Reese, TS Murphy, DL Science 183:537-8 4426930 Pubmed 1974 The localization of calcium and phosphorus in human platelets Skaer, RJ Peters, PD Emmines, JP J Cell Sci 15:679-82