BioPAX pathway converted from "p-S291-PHF20 translocates to cytosol" in the Reactome database. LEFT-TO-RIGHT p-S291-PHF20 translocates to cytosol PHF20 phosphorylated at serine S291 by AKT (Park et al. 2012, Li et al. 2013) translocates to the cytosol (Park et al. 2012). AKT thus prevents PHF20-mediated stimulation of TP53 (p53) activity (Park et al. 2012, Li et al. 2013). Authored: Orlic-Milacic, Marija, 2015-10-14 Reviewed: Zaccara, Sara, 2016-02-04 Reviewed: Inga, Alberto, 2016-02-04 Edited: Orlic-Milacic, Marija, 2015-10-14 p-S291-PHF20 Reactome DB_ID: 6805776 nucleoplasm GENE ONTOLOGY GO:0005654 UniProt:Q9BVI0 PHF20 PHF20 C20orf104 GLEA2 HCA58 NZF TZP FUNCTION Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes (By similarity). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Contributes to methyllysine-dependent p53/TP53 stabilization and up-regulation after DNA damage.SUBUNIT Homodimer; disulfide-linked. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1.TISSUE SPECIFICITY Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.DOMAIN The Tudor domain 2 mediates reading of dimethyl-lysine residues.DOMAIN The Tudor domain 1 doesn't bind dimethyl-lysine residues, due to an atypical and occluded aromatic cage.MISCELLANEOUS Antibodies against PHF20 are present in sera from patients with hepatocellular carcinoma, glioblastoma and childhood medulloblastula. Homo sapiens NCBI Taxonomy 9606 UniProt Q9BVI0 291 EQUAL O-phospho-L-serine MOD MOD:00046 1 EQUAL 1012 EQUAL Reactome Database ID Release 81 6805776 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=6805776 Reactome R-HSA-6805776 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6805776.1 Reactome http://www.reactome.org p-S291-PHF20 Reactome DB_ID: 6805794 cytosol GENE ONTOLOGY GO:0005829 1 EQUAL 1012 EQUAL Reactome Database ID Release 81 6805794 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=6805794 Reactome R-HSA-6805794 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6805794.1 Reactome Database ID Release 81 6805792 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=6805792 Reactome R-HSA-6805792 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6805792.1 22975685 Pubmed 2013 PKB-mediated PHF20 phosphorylation on Ser291 is required for p53 function in DNA damage Li, Yuwen Park, Jisoo Piao, Longzhen Kong, Gyeyeong Kim, Yongbaek Park, Kyeong Ah Zhang, Tiejun Hong, Janghee Hur, Gang Min Seok, Jeong Ho Choi, Seung-Won Yoo, Byong Chul Hemmings, BA Brazil, Derek P Kim, Seon-Hwan Park, Jongsun Cell. Signal. 25:74-84 22334668 Pubmed 2012 Identification of Akt interaction protein PHF20/TZP that transcriptionally regulates p53 Park, Sungman Kim, Donghwa Dan, Han C Chen, Huihua Testa, Joseph R Cheng, Jin Q J. Biol. Chem. 287:11151-63