BioPAX pathway converted from "pp1a cleaves itself" in the Reactome database. LEFT-TO-RIGHT 3.4.22.41 3.4.22.40 3.4.22.34 3.4.22.8 3.4.22.28 3.4.22.16 3.4.22.27 3.4.22.38 3.4.22.1 3.4.22.15 3.4.22.14 pp1a cleaves itself The crucial step of autocleavage of pp1a involves the formation of an "intermediate" pp1a dimer which has weak protease activity. This "embedded" 3CLp liberates itself by cleaving the ends off its monomer in trans. Only after that the cleaved 3CLp forms a dimer, the most efficient form of the enzyme (Hsu et al, 2005; Chen et al, 2010; Muramatsu et al, 2016). Authored: Stephan, Ralf, 2020-04-14 Reviewed: Acencio, Marcio Luis, Mazein, Alexander, 2020-05-27 Edited: D'Eustachio, Peter, 2020-05-21 pp1a Reactome DB_ID: 9684357 cytosol GENE ONTOLOGY GO:0005829 UniProt:P0C6U8 1a 1a SIMILARITY Belongs to the coronaviruses polyprotein 1ab family.CAUTION Isolates SZ3 and SZ16 have been isolated from Paguma larvata and are described as SARS-like in literature. Human SARS coronavirus NCBI Taxonomy 694009 UniProt P0C6U8 Reactome Database ID Release 81 9684357 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684357 Reactome R-COV-9684357 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-COV-9684357.1 Reactome http://www.reactome.org H2O water Reactome DB_ID: 29356 water [ChEBI:15377] water ChEBI CHEBI:15377 Reactome Database ID Release 81 29356 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=29356 Reactome R-ALL-29356 5 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-29356.5 COMPOUND C00001 additional information MI MI:0361 2 pp1a-nsp6-11 Reactome DB_ID: 9684354 3547 EQUAL 4382 EQUAL Reactome Database ID Release 81 9684354 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684354 Reactome R-COV-9684354 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-COV-9684354.1 3CL-PRO pp1a-nsp5 3C-like proteinase 1a (3241-3546) Reactome DB_ID: 9678259 3241 EQUAL 3546 EQUAL Reactome Database ID Release 81 9678259 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9678259 Reactome R-COV-9678259 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-COV-9678259.1 pp1a-nsp1-4 Reactome DB_ID: 9684311 1 EQUAL 3240 EQUAL Reactome Database ID Release 81 9684311 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684311 Reactome R-COV-9684311 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-COV-9684311.1 ACTIVATION pp1a dimer Reactome DB_ID: 9684326 2 Reactome Database ID Release 81 9684326 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684326 Reactome R-COV-9684326 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-COV-9684326.1 GENE ONTOLOGY GO:0004197 gene ontology term for cellular function MI MI:0355 Same Catalyst Activity Reactome Database ID Release 81 9684283 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684283 Reactome Database ID Release 81 9684351 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9684351 Reactome R-HSA-9684351 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-9684351.2 21203998 Pubmed 2010 Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode Chen, Shuai Jonas, Felix Shen, Can Hilgenfeld, Rolf Higenfeld, Rolf Protein Cell 1:59-74 15788388 Pubmed 2005 Mechanism of the maturation process of SARS-CoV 3CL protease Hsu, Min-Feng Kuo, Chih-Jung Chang, Kai-Ti Chang, Hui-Chuan Chou, Chia-Cheng Ko, Tzu-Ping Shr, Hui-Lin Chang, Gu-Gang Wang, Andrew H-J Liang, Po-Huang J. Biol. Chem. 280:31257-66 27799534 Pubmed 2016 SARS-CoV 3CL protease cleaves its C-terminal autoprocessing site by novel subsite cooperativity Muramatsu, Tomonari Takemoto, Chie Kim, Yong-Tae Wang, Hongfei Nishii, Wataru Terada, Takaho Shirouzu, Mikako Yokoyama, Shigeyuki Proc. Natl. Acad. Sci. U.S.A. 113:12997-13002