BioPAX pathway converted from "Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)" in the Reactome database. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> LEFT-TO-RIGHT UPF1 binds an mRNP with a termination codon preceding an Exon Junction Complex This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> Upf1 UPF1 Q9EPU0 Reactome DB_ID: 9789317 cytosol GENE ONTOLOGY GO:0005829 UniProt:Q9EPU0 Upf1 Upf1 Rent1 FUNCTION RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:27149095). Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability (By similarity). Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA hardoring long 3'UTR by inducing the NMD machinery (PubMed:27149095).SUBUNIT Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Associates with the SGM1C complex; is phosphorylated by the complex kinase component SGM1. Interacts with UPF2. Interacts with UPF3A and UPF3B. Interacts with EST1A. Interacts with SLBP. Interacts (when hyperphosphorylated) with PNRC2. Interacts with AGO1 and AGO2. Interacts with GSPT2. Interacts with isoform 1 and isoform 5 of ADAR/ADAR1. Interacts with SMG7. Interacts with ZC3H12A; this interaction occurs in a mRNA translationally active- and termination-dependent manner and is essential for ZC3H12A-mediated degradation of target mRNAs. Interacts with CPSF6. Interacts with MOV10; the interaction is direct and RNA-dependent. Interacts with SHFL; the interaction increases in the presence of RNA. Interacts with UPF2 and DDX4; interactions are mediated by TDRD6 (PubMed:27149095).TISSUE SPECIFICITY Localizes in male germ cells.DEVELOPMENTAL STAGE Weakly expressed in neonatal testes and expression increases during the development of spermatocytes and spermatids, in the late meiotic and postmeiotic stages of spermatogenesis.DOMAIN The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.PTM Phosphorylated by SMG1; required for formation of mRNA surveillance complexes.DISRUPTION PHENOTYPE Embryos are viable in pre-implantation period, show complete loss of NMD but are resorbed shortly after implantation.SIMILARITY Belongs to the DNA2/NAM7 helicase family. Mus musculus NCBI Taxonomy 10090 UniProt Q9EPU0 1 EQUAL 1129 EQUAL Reactome Database ID Release 78 9789317 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789317 Reactome R-MMU-927734 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927734.1 Reactome http://www.reactome.org SMG1:SMG8:SMG9 Complex Reactome DB_ID: 9789390 Smg1 SMG1 Q8BKX6 Reactome DB_ID: 9789388 UniProt:Q8BKX6 Smg1 UniProt Q8BKX6 1 EQUAL 3661 EQUAL Reactome Database ID Release 78 9789388 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789388 Reactome R-MMU-927760 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927760.1 1 Smg8 SMG8 Q8VE18 Reactome DB_ID: 9789380 UniProt:Q8VE18 Smg8 UniProt Q8VE18 1 EQUAL 991 EQUAL Reactome Database ID Release 78 9789380 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789380 Reactome R-MMU-927759 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927759.1 1 Smg9 SMG9 Q9DB90 Reactome DB_ID: 9789384 UniProt:Q9DB90 Smg9 UniProt Q9DB90 2 EQUAL 520 EQUAL Reactome Database ID Release 78 9789384 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789384 Reactome R-MMU-927812 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927812.1 1 Reactome Database ID Release 78 9789390 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789390 Reactome R-MMU-927853 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927853.1 Translated mRNA Complex with Premature Termination Codon Preceding Exon Junction Reactome DB_ID: 9789376 Exon Junction:UPF2:UPF3 Complex Reactome DB_ID: 9789374 Upf2 UPF2 A2AT37 Reactome DB_ID: 9789366 UniProt:A2AT37 Upf2 Upf2 FUNCTION Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.SUBUNIT Found in a post-splicing messenger ribonucleoprotein (mRNP) complex (By similarity). Associates with the exon junction complex (EJC) (By similarity). Interacts with SMG1, EST1A, UPF3A, UPF3B, EIF4A1 and EIF1 (By similarity). Interacts with UPF1; interaction is promoted by TDRD6 (PubMed:27149095). Interacts with DDX4 (PubMed:27149095).TISSUE SPECIFICITY Localized in male germ cells.DEVELOPMENTAL STAGE Weakly expressed in neonatal testes and expression increases during the development of spermatocytes and spermatids, in the late meiotic and postmeiotic stages of spermatogenesis.DISRUPTION PHENOTYPE Knockout adult liver results in hepatosteatosis and disruption of liver homeostasis. UniProt A2AT37 1 EQUAL 1272 EQUAL Reactome Database ID Release 78 9789366 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789366 Reactome R-MMU-927799 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927799.1 1 Converted from EntitySet in Reactome UPF3 Reactome DB_ID: 9789372 Upf3a UPF3A Q3ULJ3 Reactome DB_ID: 9789370 UniProt:Q3ULJ3 Upf3a UniProt Q3ULJ3 1 EQUAL 476 EQUAL Reactome Database ID Release 78 9789370 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789370 Reactome R-MMU-927801 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927801.1 Upf3b UPF3B Q3ULL6 Reactome DB_ID: 9756707 UniProt:Q3ULL6 Upf3b UniProt Q3ULL6 1 EQUAL 483 EQUAL Reactome Database ID Release 78 9756707 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756707 Reactome R-MMU-159258 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-159258.1 Reactome Database ID Release 78 9789372 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789372 Reactome R-MMU-927833 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927833.1 1 Rnps1 RNPS1 Q99M28 Reactome DB_ID: 9756703 UniProt:Q99M28 Rnps1 UniProt Q99M28 1 EQUAL 305 EQUAL Reactome Database ID Release 78 9756703 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756703 Reactome R-MMU-159256 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-159256.1 1 Core Exon Junction Complex (Core EJC) Reactome DB_ID: 9756699 Casc3 CASC3 Q8K3W3 Reactome DB_ID: 9756693 UniProt:Q8K3W3 Casc3 UniProt Q8K3W3 1 EQUAL 703 EQUAL Reactome Database ID Release 78 9756693 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756693 Reactome R-MMU-927807 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927807.1 1 Eif4a3 EIF4A3 Q91VC3 Reactome DB_ID: 9756691 UniProt:Q91VC3 Eif4a3 UniProt Q91VC3 2 EQUAL 411 EQUAL Reactome Database ID Release 78 9756691 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756691 Reactome R-MMU-927868 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927868.1 1 Magoh-Y14 complex Reactome DB_ID: 9756697 Rbm8a RBM8A Q9CWZ3 Reactome DB_ID: 9756695 UniProt:Q9CWZ3 Rbm8a UniProt Q9CWZ3 2 EQUAL 174 EQUAL Reactome Database ID Release 78 9756695 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756695 Reactome R-MMU-162462 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-162462.1 1 Converted from EntitySet in Reactome MAGOH,MAGOHB Reactome DB_ID: 9754544 Magoh MAGOH P61327 Reactome DB_ID: 9754538 nucleoplasm GENE ONTOLOGY GO:0005654 UniProt:P61327 Magoh UniProt P61327 1 EQUAL 146 EQUAL Reactome Database ID Release 78 9754538 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754538 Reactome R-MMU-156658 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-156658.1 Magohb MAGOHB Q9CQL1 Reactome DB_ID: 9754542 UniProt:Q9CQL1 Magohb UniProt Q9CQL1 2 EQUAL 148 EQUAL Reactome Database ID Release 78 9754542 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754542 Reactome R-MMU-8867888 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8867888.1 Reactome Database ID Release 78 9754544 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754544 Reactome R-MMU-8868752 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868752.1 1 Reactome Database ID Release 78 9756697 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756697 Reactome R-MMU-162463 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-162463.1 1 Reactome Database ID Release 78 9756699 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756699 Reactome R-MMU-927869 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927869.1 1 Reactome Database ID Release 78 9789374 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789374 Reactome R-MMU-927798 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927798.1 1 80S ribosome Reactome DB_ID: 9755053 60S ribosomal complex Reactome DB_ID: 9755051 Rpl18 RPL18 P35980 Reactome DB_ID: 9754934 UniProt:P35980 Rpl18 Rpl18 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eL18 family. UniProt P35980 2 EQUAL 188 EQUAL Reactome Database ID Release 78 9754934 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754934 Reactome R-MMU-72420 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72420.1 1 Rpl12 60S ribosomal protein L12 RL12_MOUSE Reactome DB_ID: 9633470 UniProt:P35979 Rpl12 Rpl12 FUNCTION Binds directly to 26S ribosomal RNA.SIMILARITY Belongs to the universal ribosomal protein uL11 family. UniProt P35979 1 EQUAL 165 EQUAL Reactome Database ID Release 78 9633470 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633470 Reactome R-MMU-9633470 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633470.1 1 Converted from EntitySet in Reactome RPL3,RPL3L Reactome DB_ID: 9755025 Rpl3 RPL3 P27659 Reactome DB_ID: 9755019 UniProt:P27659 Rpl3 Rpl3 FUNCTION The L3 protein is a component of the large subunit of cytoplasmic ribosomes.SUBUNIT Component of the large ribosomal subunit. Interacts with METTL18. Interacts with DHX33 (PubMed:26100019).SIMILARITY Belongs to the universal ribosomal protein uL3 family. UniProt P27659 2 EQUAL 403 EQUAL Reactome Database ID Release 78 9755019 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755019 Reactome R-MMU-72448 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72448.1 Rpl3l RPL3L E9PWZ3 Reactome DB_ID: 9755023 UniProt:E9PWZ3 Rpl3l UniProt E9PWZ3 1 EQUAL 407 EQUAL Reactome Database ID Release 78 9755023 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755023 Reactome R-MMU-72450 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72450.1 Reactome Database ID Release 78 9755025 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755025 Reactome R-MMU-8868851 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868851.1 1 Rpl35 RPL35 Q6ZWV7 Reactome DB_ID: 9754961 UniProt:Q6ZWV7 Rpl35 Rpl35 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the universal ribosomal protein uL29 family. UniProt Q6ZWV7 2 EQUAL 123 EQUAL Reactome Database ID Release 78 9754961 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754961 Reactome R-MMU-72460 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72460.1 1 Rpl21 RPL21 O09167 Reactome DB_ID: 9754939 UniProt:O09167 Rpl21 Rpl21 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eL21 family. UniProt O09167 2 EQUAL 160 EQUAL Reactome Database ID Release 78 9754939 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754939 Reactome R-MMU-72426 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72426.1 1 Rplp1 60S acidic ribosomal protein P1 RLA1_MOUSE Reactome DB_ID: 9633567 UniProt:P47955 Rplp1 Rplp1 FUNCTION Plays an important role in the elongation step of protein synthesis.SUBUNIT Heterodimer with RPLP2 at the lateral ribosomal stalk of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein P1/P2 family. UniProt P47955 2 EQUAL 114 EQUAL Reactome Database ID Release 78 9633567 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633567 Reactome R-MMU-9633567 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633567.1 1 Rpl23 60S ribosomal protein L23 RL23_MOUSE Reactome DB_ID: 9633465 UniProt:P62830 Rpl23 Rpl23 SIMILARITY Belongs to the universal ribosomal protein uL14 family. UniProt P62830 1 EQUAL 140 EQUAL Reactome Database ID Release 78 9633465 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633465 Reactome R-MMU-9633465 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633465.1 1 Rpl36 RPL36 P47964 Reactome DB_ID: 9754965 UniProt:P47964 Rpl36 Rpl36 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eL36 family. UniProt P47964 2 EQUAL 105 EQUAL Reactome Database ID Release 78 9754965 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754965 Reactome R-MMU-72464 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72464.1 1 Rpl17 RPL17 Q9CPR4 Reactome DB_ID: 9754931 UniProt:Q9CPR4 Rpl17 Rpl17 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the universal ribosomal protein uL22 family. UniProt Q9CPR4 2 EQUAL 184 EQUAL Reactome Database ID Release 78 9754931 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754931 Reactome R-MMU-72418 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72418.1 1 Uba52 RPL40 P62984 Reactome DB_ID: 9754979 UniProt:P62984 Uba52 Uba52 Ubcep2 SUBUNIT Ribosomal protein L40 is part of the 60S ribosomal subunit. Interacts with UBQLN1 (via UBA domain).MISCELLANEOUS Ubiquitin is encoded by 4 different genes. Uba52 and Rps27a genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.SIMILARITY In the N-terminal section; belongs to the ubiquitin family.SIMILARITY In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family. UniProt P62984 77 EQUAL 128 EQUAL Reactome Database ID Release 78 9754979 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754979 Reactome R-MMU-72476 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72476.1 1 Rpl37a RPL37A P61514 Reactome DB_ID: 9754971 UniProt:P61514 Rpl37a Rpl37a SIMILARITY Belongs to the eukaryotic ribosomal protein eL43 family. UniProt P61514 2 EQUAL 92 EQUAL Reactome Database ID Release 78 9754971 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754971 Reactome R-MMU-72468 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72468.1 1 Rpl29 RPL29 P47915 Reactome DB_ID: 9754950 UniProt:P47915 Rpl29 Rpl29 Rpl43 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eL29 family. UniProt P47915 2 EQUAL 159 EQUAL Reactome Database ID Release 78 9754950 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754950 Reactome R-MMU-72446 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72446.1 1 Rplp0 60S acidic ribosomal protein P0 RLA0_MOUSE Reactome DB_ID: 9633527 UniProt:P14869 Rplp0 Rplp0 Arbp FUNCTION Ribosomal protein P0 is the functional equivalent of E.coli protein L10.SUBUNIT P0 forms a pentameric complex by interaction with dimers of P1 and P2. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APEX1. Interacts with FMR1.SIMILARITY Belongs to the universal ribosomal protein uL10 family. UniProt P14869 1 EQUAL 317 EQUAL Reactome Database ID Release 78 9633527 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633527 Reactome R-MMU-9633527 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633527.1 1 Rpl19 60S ribosomal protein L19 RL19_MOUSE Reactome DB_ID: 9633588 UniProt:P84099 Rpl19 Rpl19 PTM Citrullinated by PADI4.SIMILARITY Belongs to the eukaryotic ribosomal protein eL19 family. UniProt P84099 1 EQUAL 196 EQUAL Reactome Database ID Release 78 9633588 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633588 Reactome R-MMU-9633588 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633588.1 1 Rpl28 60S ribosomal protein L28 RL28_MOUSE Reactome DB_ID: 9633568 UniProt:P41105 Rpl28 Rpl28 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eL28 family. UniProt P41105 2 EQUAL 137 EQUAL Reactome Database ID Release 78 9633568 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633568 Reactome R-MMU-9633568 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633568.1 1 Rpl24 60S ribosomal protein L24 RL24_MOUSE Reactome DB_ID: 9633542 UniProt:Q8BP67 Rpl24 Rpl24 SIMILARITY Belongs to the eukaryotic ribosomal protein eL24 family. UniProt Q8BP67 1 EQUAL 157 EQUAL Reactome Database ID Release 78 9633542 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633542 Reactome R-MMU-9633542 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633542.1 1 Rpl37 RPL37 Q9D823 Reactome DB_ID: 9754968 UniProt:Q9D823 Rpl37 Rpl37 FUNCTION Binds to the 23S rRNA.SIMILARITY Belongs to the eukaryotic ribosomal protein eL37 family. UniProt Q9D823 2 EQUAL 97 EQUAL Reactome Database ID Release 78 9754968 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754968 Reactome R-MMU-72466 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72466.1 1 Converted from EntitySet in Reactome RPL39,RPL39L Reactome DB_ID: 9755049 Rpl39 RPL39 P62892 Reactome DB_ID: 9755043 UniProt:P62892 Rpl39 Rpl39 SUBUNIT Interacts with IMPACT (PubMed:22404850).MISCELLANEOUS This protein is the smallest and one of the most basic of the proteins in liver ribosomes.SIMILARITY Belongs to the eukaryotic ribosomal protein eL39 family. UniProt P62892 2 EQUAL 51 EQUAL Reactome Database ID Release 78 9755043 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755043 Reactome R-MMU-72472 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72472.1 Rpl39l RPL39L Q9CQD0 Reactome DB_ID: 9755047 UniProt:Q9CQD0 Rpl39l UniProt Q9CQD0 1 EQUAL 51 EQUAL Reactome Database ID Release 78 9755047 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755047 Reactome R-MMU-8868836 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868836.1 Reactome Database ID Release 78 9755049 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755049 Reactome R-MMU-8868812 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868812.1 1 Rpl27 60S ribosomal protein L27 RL27_MOUSE Reactome DB_ID: 9633491 UniProt:P61358 Rpl27 Rpl27 FUNCTION Component of the large ribosomal subunit (By similarity). Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs (By similarity).SUBUNIT Component of the large ribosomal subunit (By similarity). Interacts with RRP1B (By similarity). Interacts with DHX33 (PubMed:26100019).SIMILARITY Belongs to the eukaryotic ribosomal protein eL27 family. UniProt P61358 1 EQUAL 136 EQUAL Reactome Database ID Release 78 9633491 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633491 Reactome R-MMU-9633491 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633491.1 1 Rpl23a 60S ribosomal protein L23a RL23A_MOUSE Reactome DB_ID: 9633537 UniProt:P62751 Rpl23a Rpl23a FUNCTION Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds a specific region on the 26S rRNA (By similarity). May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination (By similarity).SUBUNIT Interacts with LYAR and GNL2. Interacts with MDM2; this interaction may promote MDM2-mediated p53/TP53 polyubiquitination.PTM N-terminus is methylated by METTL11A/NTM1.PTM Citrullinated by PADI4.SIMILARITY Belongs to the universal ribosomal protein uL23 family. UniProt P62751 2 EQUAL 156 EQUAL Reactome Database ID Release 78 9633537 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633537 Reactome R-MMU-9633537 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633537.1 1 Ghost homologue of RPL41 Reactome DB_ID: 9754981 Reactome Database ID Release 78 9754981 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754981 Reactome R-MMU-72478 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72478.1 1 Rplp2 60S acidic ribosomal protein P2 RLA2_MOUSE Reactome DB_ID: 9633556 UniProt:P99027 Rplp2 Rplp2 FUNCTION Plays an important role in the elongation step of protein synthesis.SUBUNIT Heterodimer with RPLP1 at the lateral ribosomal stalk of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein P1/P2 family. UniProt P99027 1 EQUAL 115 EQUAL Reactome Database ID Release 78 9633556 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633556 Reactome R-MMU-9633556 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633556.1 1 Rpl4 RPL4 Q9D8E6 Reactome DB_ID: 9754977 UniProt:Q9D8E6 Rpl4 Rpl4 SUBUNIT May bind IPO9 with low affinity. Interacts with RBM3 (By similarity).PTM Citrullinated by PADI4.SIMILARITY Belongs to the universal ribosomal protein uL4 family. UniProt Q9D8E6 2 EQUAL 427 EQUAL Reactome Database ID Release 78 9754977 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754977 Reactome R-MMU-72474 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72474.1 1 Rpl9 60S ribosomal protein L9 RL9_MOUSE Reactome DB_ID: 9633585 UniProt:P51410 Rpl9 Rpl9 SIMILARITY Belongs to the universal ribosomal protein uL6 family. UniProt P51410 1 EQUAL 192 EQUAL Reactome Database ID Release 78 9633585 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633585 Reactome R-MMU-9633585 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633585.1 1 Rpl11 60S ribosomal protein L11 RL11_MOUSE Reactome DB_ID: 9633593 UniProt:Q9CXW4 Rpl11 Rpl11 FUNCTION Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:21804542). Promotes nucleolar location of PML (PubMed:15195100).SUBUNIT Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11 (By similarity). Interacts with PML (PubMed:15195100). Interacts with MDM2; negatively regulates MDM2-mediated TP53 ubiquitination and degradation (PubMed:15195100, PubMed:21804542). Interacts with NOP53; retains RPL11 into the nucleolus (PubMed:21804542).SIMILARITY Belongs to the universal ribosomal protein uL5 family. UniProt Q9CXW4 2 EQUAL 178 EQUAL Reactome Database ID Release 78 9633593 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633593 Reactome R-MMU-9633593 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633593.1 1 Rpl5 60S ribosomal protein L5 RL5_MOUSE Reactome DB_ID: 9633557 UniProt:P47962 Rpl5 Rpl5 FUNCTION Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Interacts with RRP1B.SUBUNIT Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11. Interacts with NVL in an ATP-dependent manner.SIMILARITY Belongs to the universal ribosomal protein uL18 family. UniProt P47962 2 EQUAL 297 EQUAL Reactome Database ID Release 78 9633557 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633557 Reactome R-MMU-9633557 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633557.1 1 Rpl7 RPL7 P14148 Reactome DB_ID: 9754988 UniProt:P14148 Rpl7 Rpl7 FUNCTION Component of the large ribosomal subunit (By similarity). Binds to G-rich structures in 28S rRNA and in mRNAs. Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs (By similarity).SUBUNIT Component of the large ribosomal subunit. Homodimer. Interacts with DHX33 (PubMed:26100019).SIMILARITY Belongs to the universal ribosomal protein uL30 family. UniProt P14148 1 EQUAL 248 EQUAL Reactome Database ID Release 78 9754988 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754988 Reactome R-MMU-72486 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72486.1 1 Converted from EntitySet in Reactome RPL36A,RPL36AL Reactome DB_ID: 9755040 Rpl36a RPL36A P83882 Reactome DB_ID: 9755028 UniProt:P83882 Rpl36a Rpl36a Rpl44 SIMILARITY Belongs to the eukaryotic ribosomal protein eL42 family. UniProt P83882 2 EQUAL 106 EQUAL Reactome Database ID Release 78 9755028 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755028 Reactome R-MMU-72480 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72480.1 Converted from EntitySet in Reactome Homologues of RPL36AL Reactome DB_ID: 9755038 Gm6525 RPL36AL A0A0A6YW33 Reactome DB_ID: 9755032 UniProt:A0A0A6YW33 Gm6525 UniProt A0A0A6YW33 1 EQUAL 106 EQUAL Reactome Database ID Release 78 9755032 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755032 Reactome R-MMU-8868805 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868805.1 RPL36AL Rpl36a-ps1 A0A2I3BPG9 Reactome DB_ID: 9755036 UniProt:A0A2I3BPG9 Rpl36a-ps1 UniProt A0A2I3BPG9 1 EQUAL 106 EQUAL Reactome Database ID Release 78 9755036 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755036 Reactome R-MMU-8868805-2 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868805-2.1 Rpl36a 60S ribosomal protein L36a RL36A_MOUSE Reactome DB_ID: 9633517 1 EQUAL 106 EQUAL Reactome Database ID Release 78 9633517 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633517 Reactome R-MMU-9633517 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633517.1 Reactome Database ID Release 78 9755038 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755038 Reactome R-MMU-8868805-3 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868805-3.1 Reactome Database ID Release 78 9755040 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755040 Reactome R-MMU-8868819 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868819.1 1 Rpl18a 60S ribosomal protein L18a RL18A_MOUSE Reactome DB_ID: 9633555 UniProt:P62717 Rpl18a Rpl18a SUBUNIT Binds IPO9 with high affinity.SIMILARITY Belongs to the eukaryotic ribosomal protein eL20 family. UniProt P62717 1 EQUAL 176 EQUAL Reactome Database ID Release 78 9633555 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633555 Reactome R-MMU-9633555 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633555.1 1 Rpl14 RPL14 Q9CR57 Reactome DB_ID: 9754925 UniProt:Q9CR57 Rpl14 Rpl14 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eL14 family. UniProt Q9CR57 2 EQUAL 215 EQUAL Reactome Database ID Release 78 9754925 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754925 Reactome R-MMU-72414 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72414.1 1 Rpl26 60S ribosomal protein L26 RL26_MOUSE Reactome DB_ID: 9633487 UniProt:P61255 Rpl26 Rpl26 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit (Probable). Interacts with DHX33 (PubMed:26100019).INDUCTION Up-regulated in silica-treated macrophages.PTM Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets.SIMILARITY Belongs to the universal ribosomal protein uL24 family. UniProt P61255 1 EQUAL 145 EQUAL Reactome Database ID Release 78 9633487 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633487 Reactome R-MMU-9633487 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633487.1 1 Rpl13 60S ribosomal protein L13 RL13_MOUSE Reactome DB_ID: 9633507 UniProt:P47963 Rpl13 Rpl13 SIMILARITY Belongs to the eukaryotic ribosomal protein eL13 family. UniProt P47963 1 EQUAL 211 EQUAL Reactome Database ID Release 78 9633507 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633507 Reactome R-MMU-9633507 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633507.1 1 Rpl34 RPL34 Q9D1R9 Reactome DB_ID: 9754958 UniProt:Q9D1R9 Rpl34 Rpl34 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eL34 family. UniProt Q9D1R9 2 EQUAL 117 EQUAL Reactome Database ID Release 78 9754958 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754958 Reactome R-MMU-72458 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72458.1 1 Rpl35a 60S ribosomal protein L35a RL35A_MOUSE Reactome DB_ID: 9633591 UniProt:O55142 Rpl35a Rpl35a FUNCTION Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.SIMILARITY Belongs to the eukaryotic ribosomal protein eL33 family. UniProt O55142 1 EQUAL 110 EQUAL Reactome Database ID Release 78 9633591 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633591 Reactome R-MMU-9633591 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633591.1 1 Rpl10a 60S ribosomal protein L10a RL10A_MOUSE Reactome DB_ID: 9633563 UniProt:P53026 Rpl10a Rpl10a Nedd-6 Nedd6 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit.SIMILARITY Belongs to the universal ribosomal protein uL1 family. UniProt P53026 2 EQUAL 217 EQUAL Reactome Database ID Release 78 9633563 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633563 Reactome R-MMU-9633563 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633563.1 1 Rpl27a RPL27A P14115 Reactome DB_ID: 9754946 UniProt:P14115 Rpl27a Rpl27a PTM Hydroxylated on His-39 by MINA.SIMILARITY Belongs to the universal ribosomal protein uL15 family. UniProt P14115 2 EQUAL 148 EQUAL Reactome Database ID Release 78 9754946 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754946 Reactome R-MMU-72442 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72442.1 1 Rpl15 RPL15 Q9CZM2 Reactome DB_ID: 9754928 UniProt:Q9CZM2 Rpl15 Rpl15 SUBUNIT Interacts with IFIT1 (via TPR repeats 1-4).SIMILARITY Belongs to the eukaryotic ribosomal protein eL15 family. UniProt Q9CZM2 1 EQUAL 204 EQUAL Reactome Database ID Release 78 9754928 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754928 Reactome R-MMU-72416 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72416.1 1 Rpl30 60S ribosomal protein L30 RL30_MOUSE Reactome DB_ID: 9633581 UniProt:P62889 Rpl30 Rpl30 SIMILARITY Belongs to the eukaryotic ribosomal protein eL30 family. UniProt P62889 1 EQUAL 115 EQUAL Reactome Database ID Release 78 9633581 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633581 Reactome R-MMU-9633581 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633581.1 1 Rpl7a RPL7A P12970 Reactome DB_ID: 9754991 UniProt:P12970 Rpl7a Rpl7a Surf-3 Surf3 SUBUNIT Interacts with CRY1 (PubMed:19129230). Interacts with DICER1, AGO2, TARBP2, MOV10 and EIF6; they form a large RNA-induced silencing complex (RISC) (By similarity).SIMILARITY Belongs to the eukaryotic ribosomal protein eL8 family. UniProt P12970 2 EQUAL 266 EQUAL Reactome Database ID Release 78 9754991 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754991 Reactome R-MMU-72488 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72488.1 1 Rpl6 RPL6 P47911 Reactome DB_ID: 9754985 UniProt:P47911 Rpl6 Rpl6 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit (By similarity). May bind IPO9 with low affinity (PubMed:11823430).SIMILARITY Belongs to the eukaryotic ribosomal protein eL6 family. UniProt P47911 2 EQUAL 288 EQUAL Reactome Database ID Release 78 9754985 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754985 Reactome R-MMU-72484 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72484.1 1 Rpl38 RPL38 Q9JJI8 Reactome DB_ID: 9754974 UniProt:Q9JJI8 Rpl38 Rpl38 SIMILARITY Belongs to the eukaryotic ribosomal protein eL38 family. UniProt Q9JJI8 2 EQUAL 70 EQUAL Reactome Database ID Release 78 9754974 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754974 Reactome R-MMU-72470 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72470.1 1 Ghost homologue of 5.8S rRNA Reactome DB_ID: 9754999 Reactome Database ID Release 78 9754999 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754999 Reactome R-MMU-72496 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72496.1 1 Converted from EntitySet in Reactome RPL10,RPL10L Reactome DB_ID: 9755009 Rpl10 RPL10 Q6ZWV3 Reactome DB_ID: 9755004 UniProt:Q6ZWV3 Rpl10 Rpl10 Qm FUNCTION Component of the large ribosomal subunit. Plays a role in the formation of actively translating ribosomes. May play a role in the embryonic brain development.SUBUNIT Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S).PTM Citrullinated by PADI4.PTM Ufmylated by UFL1.SIMILARITY Belongs to the universal ribosomal protein uL16 family. UniProt Q6ZWV3 2 EQUAL 214 EQUAL Reactome Database ID Release 78 9755004 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755004 Reactome R-MMU-72402 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72402.1 Rpl10l RPL10L P86048 Reactome DB_ID: 9755007 UniProt:P86048 Rpl10l Rpl10l SIMILARITY Belongs to the universal ribosomal protein uL16 family. UniProt P86048 2 EQUAL 214 EQUAL Reactome Database ID Release 78 9755007 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755007 Reactome R-MMU-8868799 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868799.1 Reactome Database ID Release 78 9755009 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755009 Reactome R-MMU-8868854 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868854.1 1 Converted from EntitySet in Reactome RPL22,RPL22L1 Reactome DB_ID: 9755015 Rpl22 RPL22 P67984 Reactome DB_ID: 9755012 UniProt:P67984 Rpl22 Rpl22 MISCELLANEOUS Binds to Epstein-Barr virus small RNAs and to heparin.SIMILARITY Belongs to the eukaryotic ribosomal protein eL22 family. UniProt P67984 2 EQUAL 128 EQUAL Reactome Database ID Release 78 9755012 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755012 Reactome R-MMU-72428 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72428.1 Rpl22l1 60S ribosomal protein L22-like 1 RL22L_MOUSE Reactome DB_ID: 9633587 UniProt:Q9D7S7 Rpl22l1 Rpl22l1 SIMILARITY Belongs to the eukaryotic ribosomal protein eL22 family. UniProt Q9D7S7 1 EQUAL 122 EQUAL Reactome Database ID Release 78 9633587 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633587 Reactome R-MMU-9633587 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633587.1 Reactome Database ID Release 78 9755015 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755015 Reactome R-MMU-8868847 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868847.1 1 Rpl32 RPL32 P62911 Reactome DB_ID: 9754955 UniProt:P62911 Rpl32 Rpl32 SIMILARITY Belongs to the eukaryotic ribosomal protein eL32 family. UniProt P62911 2 EQUAL 135 EQUAL Reactome Database ID Release 78 9754955 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754955 Reactome R-MMU-72456 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72456.1 1 Rpl13a 60S ribosomal protein L13a RL13A_MOUSE Reactome DB_ID: 9633578 UniProt:P19253 Rpl13a Rpl13a P198 Tstap198-7 FUNCTION Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex.SUBUNIT Component of the 60S ribosome. Component of the GAIT complex. Interacts with EIF4G1 (By similarity).PTM Phosphorylation at Ser-77 upon interferon-gamma treatment in macrophages involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition.PTM Citrullinated by PADI4.POLYMORPHISM The antigenic allele of P198 differs from the normal allele by a single mutation. The TUM- mutation P198 generates a new epitope recognized by syngeneic T-cells.SIMILARITY Belongs to the universal ribosomal protein uL13 family. UniProt P19253 2 EQUAL 203 EQUAL Reactome Database ID Release 78 9633578 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633578 Reactome R-MMU-9633578 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633578.1 1 Rpl31 60S ribosomal protein L31 RL31_MOUSE Reactome DB_ID: 9633571 UniProt:P62900 Rpl31 Rpl31 SIMILARITY Belongs to the eukaryotic ribosomal protein eL31 family. UniProt P62900 1 EQUAL 125 EQUAL Reactome Database ID Release 78 9633571 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633571 Reactome R-MMU-9633571 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633571.1 1 Rpl8 RPL8 P62918 Reactome DB_ID: 9754994 UniProt:P62918 Rpl8 Rpl8 FUNCTION Component of the large ribosomal subunit.SUBUNIT Component of the large ribosomal subunit (By similarity). Interacts with CRY1 (PubMed:19129230).PTM Hydroxylated on His-216 by RIOX1. The modification is impaired by hypoxia.SIMILARITY Belongs to the universal ribosomal protein uL2 family. UniProt P62918 2 EQUAL 257 EQUAL Reactome Database ID Release 78 9754994 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754994 Reactome R-MMU-72490 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72490.1 1 Ghost homologue of 28S rRNA Reactome DB_ID: 9755001 Reactome Database ID Release 78 9755001 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755001 Reactome R-MMU-72498 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72498.1 1 Ghost homologue of 5S rRNA Reactome DB_ID: 9754997 Reactome Database ID Release 78 9754997 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754997 Reactome R-MMU-72494 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72494.1 1 Reactome Database ID Release 78 9755051 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755051 Reactome R-MMU-72499 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72499.1 1 40S ribosomal complex Reactome DB_ID: 9754743 Ghost homologue of FAU Reactome DB_ID: 9754705 Reactome Database ID Release 78 9754705 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754705 Reactome R-MMU-72371 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72371.1 1 Rps24 40S ribosomal protein S24 RS24_MOUSE Reactome DB_ID: 9633577 UniProt:P62849 Rps24 Rps24 FUNCTION Required for processing of pre-rRNA and maturation of 40S ribosomal subunits.INDUCTION Down-regulated during adipocyte differentiation and up-regulated during cellular transformation.SIMILARITY Belongs to the eukaryotic ribosomal protein eS24 family. UniProt P62849 1 EQUAL 133 EQUAL Reactome Database ID Release 78 9633577 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633577 Reactome R-MMU-9633577 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633577.1 1 Ghost homologue of RPS27A(77-156) Reactome DB_ID: 9754698 Reactome Database ID Release 78 9754698 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754698 Reactome R-MMU-72363 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72363.1 1 Rps3 40S ribosomal protein S3 ecNumber evidence="8"4.2.99.18/ecNumber RS3_MOUSE Reactome DB_ID: 9633584 UniProt:P62908 Rps3 Rps3 FUNCTION Involved in translation as a component of the 40S small ribosomal subunit (By similarity). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (By similarity). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (By similarity). Has also been shown to bind with similar affinity to intact and damaged DNA (By similarity). Stimulates the N-glycosylase activity of the base excision protein OGG1 (By similarity). Enhances the uracil excision activity of UNG1 (By similarity). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (By similarity). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (By similarity). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (By similarity). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (By similarity). Represses its own translation by binding to its cognate mRNA (By similarity). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (By similarity). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (By similarity). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (By similarity). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (By similarity).SUBUNIT Component of the 40S small ribosomal subunit. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with HNRPD. Interacts with PRMT1; the interaction methylates RPS3. Interacts with SUMO1; the interaction sumoylates RPS3. Interacts with UBC9. Interacts with CDK1; the interaction phosphorylates RPS3. Interacts with PRKCD; the interaction phosphorylates RPS3. Interacts with PKB/AKT; the interaction phosphorylates RPS3. Interacts with E2F1; the interaction occurs in the absence of nerve growth factor and increases transcription of pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5. Interacts with the base excision repair proteins APEX1 and OGG1; interaction with OGG1 increases OGG1 N-glycosylase activity. Interacts with UNG; the interaction increases the uracil excision activity of UNG1. Interacts with HSP90; the interaction prevents the ubiquitination and proteasome-dependent degradation of RPS3 and is suppressed by increased ROS levels. Interacts with TOM70; the interaction promotes translocation of RPS3 to the mitochondrion. Interacts (via N-terminus) with RELA (via N-terminus); the interaction enhances the DNA-binding activity of the NF-kappa-B p65-p50 complex. Interacts with NFKBIA; the interaction is direct and may bridge the interaction between RPS3 and RELA. Interacts with IKKB; the interaction phosphorylates RPS3 and enhances its translocation to the nucleus. Interacts (via KH domain) with MDM2 and TP53. Interacts with TRADD. Interacts with CRY1.PTM Methylation by PRMT1 is required for import into the nucleolus and for ribosome assembly.PTM Sumoylation by SUMO1 enhances protein stability through increased resistance to proteolysis. Sumoylation occurs at one or more of the three consensus sites, Lys-18, Lys-214 and Lys-230.PTM Phosphorylation at Thr-221 by CDK1 occurs mainly in G2/M phase. Phosphorylation by PRKCD occurs on a non-ribosomal-associated form which results in translocation of RPS3 to the nucleus and enhances its endonuclease activity. Phosphorylated on Ser-209 by IKKB in response to activation of the NF-kappa-B p65-p50 complex which enhances the association of RPS3 with importin-alpha and mediates the nuclear translocation of RPS3. Phosphorylation by MAPK is required for translocation to the nucleus following exposure of cells to DNA damaging agents such as hydrogen peroxide. Phosphorylation by PKB/AKT mediates RPS3 nuclear translocation, enhances RPS3 endonuclease activity and suppresses RPS3-induced neuronal apoptosis.PTM Ubiquitinated. This is prevented by interaction with HSP90 which stabilizes the protein. Monoubiquitinated at Lys-214 by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.PTM Ufmylated by UFL1.SIMILARITY Belongs to the universal ribosomal protein uS3 family. UniProt P62908 2 EQUAL 243 EQUAL Reactome Database ID Release 78 9633584 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633584 Reactome R-MMU-9633584 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633584.1 1 Rps25 40S ribosomal protein S25 RS25_MOUSE Reactome DB_ID: 9633529 UniProt:P62852 Rps25 Rps25 SIMILARITY Belongs to the eukaryotic ribosomal protein eS25 family. UniProt P62852 1 EQUAL 125 EQUAL Reactome Database ID Release 78 9633529 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633529 Reactome R-MMU-9633529 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633529.1 1 Rps15 40S ribosomal protein S15 RS15_MOUSE Reactome DB_ID: 9633500 UniProt:P62843 Rps15 Rps15 Rig SIMILARITY Belongs to the universal ribosomal protein uS19 family. UniProt P62843 2 EQUAL 145 EQUAL Reactome Database ID Release 78 9633500 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633500 Reactome R-MMU-9633500 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633500.1 1 Rps15a RPS15A P62245 Reactome DB_ID: 9754683 UniProt:P62245 Rps15a Rps15a FUNCTION Structural component of the ribosome. Required for proper erythropoiesis.SUBUNIT Component of the 40S ribosomal subunit.SIMILARITY Belongs to the universal ribosomal protein uS8 family. UniProt P62245 2 EQUAL 130 EQUAL Reactome Database ID Release 78 9754683 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754683 Reactome R-MMU-72345 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72345.1 1 Rpsa fullName evidence="3"40S ribosomal protein SA RSSA_MOUSE Reactome DB_ID: 9633498 UniProt:P14206 Rpsa Rpsa Lamr1 P40-8 FUNCTION Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. Acts as a PPP1R16B-dependent substrate of PPP1CA (By similarity). Enables malignant tumor cells to penetrate laminin tissue and vessel barriers. Activates precursor thymic anti-OFA/iLRP specific cytotoxic T-cell. May induce CD8 T-suppressor cells secreting IL-10.SUBUNIT Monomer (37LRP) and homodimer (67LR) (By similarity). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21 (By similarity). Interacts with several laminins including at least LAMB1. Interacts with MDK. Interacts with PRNP. The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B (By similarity).PTM Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.PTM Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.MISCELLANEOUS This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage.MISCELLANEOUS It is thought that in vertebrates 37/67 kDa laminin receptor acquired a dual function during evolution. It developed from the ribosomal protein SA, playing an essential role in the protein biosynthesis lacking any laminin binding activity, to a cell surface receptor with laminin binding activity.SIMILARITY Belongs to the universal ribosomal protein uS2 family. UniProt P14206 2 EQUAL 295 EQUAL Reactome Database ID Release 78 9633498 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633498 Reactome R-MMU-9633498 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633498.1 1 Converted from EntitySet in Reactome RPS27,RPS27L Reactome DB_ID: 9754726 Rps27 RPS27 Q6ZWU9 Reactome DB_ID: 9754721 UniProt:Q6ZWU9 Rps27 Rps27 FUNCTION Component of the small ribosomal subunit (By similarity). Required for proper rRNA processing and maturation of 18S rRNAs (By similarity).SUBUNIT Component of the small ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eS27 family. UniProt Q6ZWU9 2 EQUAL 84 EQUAL Reactome Database ID Release 78 9754721 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754721 Reactome R-MMU-72361 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72361.1 Rps27l RPS27L Q6ZWY3 Reactome DB_ID: 9754724 UniProt:Q6ZWY3 Rps27l Rps27l SIMILARITY Belongs to the eukaryotic ribosomal protein eS27 family. UniProt Q6ZWY3 2 EQUAL 84 EQUAL Reactome Database ID Release 78 9754724 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754724 Reactome R-MMU-8868849 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868849.1 Reactome Database ID Release 78 9754726 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754726 Reactome R-MMU-8868839 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868839.1 1 Rps23 RPS23 P62267 Reactome DB_ID: 9754691 UniProt:P62267 Rps23 Rps23 FUNCTION Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Plays an important role in translational accuracy.SUBUNIT Component of the 40S small ribosomal subunit.PTM Hydroxylation at Pro-62 affects translation termination efficiency.SIMILARITY Belongs to the universal ribosomal protein uS12 family. UniProt P62267 2 EQUAL 143 EQUAL Reactome Database ID Release 78 9754691 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754691 Reactome R-MMU-72353 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72353.1 1 Rps10 40S ribosomal protein S10 RS10_MOUSE Reactome DB_ID: 9633546 UniProt:P63325 Rps10 Rps10 FUNCTION Component of the 40S ribosomal subunit.SUBUNIT Component of the small ribosomal subunit. Interacts with PRMT5. The methylated form interacts with NPM1 (By similarity).PTM Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation (By similarity).PTM Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.SIMILARITY Belongs to the eukaryotic ribosomal protein eS10 family. UniProt P63325 1 EQUAL 165 EQUAL Reactome Database ID Release 78 9633546 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633546 Reactome R-MMU-9633546 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633546.1 1 Rps26 RPS26 P62855 Reactome DB_ID: 9754696 UniProt:P62855 Rps26 Rps26 SUBUNIT Component of the 40S small ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eS26 family. UniProt P62855 2 EQUAL 115 EQUAL Reactome Database ID Release 78 9754696 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754696 Reactome R-MMU-72359 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72359.1 1 Rps14 RPS14 P62264 Reactome DB_ID: 9754669 UniProt:P62264 Rps14 Rps14 SIMILARITY Belongs to the universal ribosomal protein uS11 family. UniProt P62264 2 EQUAL 151 EQUAL Reactome Database ID Release 78 9754669 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754669 Reactome R-MMU-72333 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72333.1 1 Rps5 40S ribosomal protein S5 component recommendedName: 40S ribosomal protein S5, N-terminally processed /component RS5_MOUSE Reactome DB_ID: 9633547 UniProt:P97461 Rps5 Rps5 SIMILARITY Belongs to the universal ribosomal protein uS7 family. UniProt P97461 1 EQUAL 204 EQUAL Reactome Database ID Release 78 9633547 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633547 Reactome R-MMU-9633547 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633547.1 1 Rps13 RPS13 P62301 Reactome DB_ID: 9754666 UniProt:P62301 Rps13 Rps13 SIMILARITY Belongs to the universal ribosomal protein uS15 family. UniProt P62301 2 EQUAL 151 EQUAL Reactome Database ID Release 78 9754666 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754666 Reactome R-MMU-72331 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72331.1 1 Rps11 40S ribosomal protein S11 RS11_MOUSE Reactome DB_ID: 9633582 UniProt:P62281 Rps11 Rps11 PTM Citrullinated by PADI4.SIMILARITY Belongs to the universal ribosomal protein uS17 family. UniProt P62281 2 EQUAL 158 EQUAL Reactome Database ID Release 78 9633582 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633582 Reactome R-MMU-9633582 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633582.1 1 Rps16 RPS16 P14131 Reactome DB_ID: 9754673 UniProt:P14131 Rps16 Rps16 SIMILARITY Belongs to the universal ribosomal protein uS9 family. UniProt P14131 2 EQUAL 146 EQUAL Reactome Database ID Release 78 9754673 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754673 Reactome R-MMU-72337 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72337.1 1 Rps29 RPS29 P62274 Reactome DB_ID: 9754702 UniProt:P62274 Rps29 Rps29 SUBUNIT Component of the 40S small ribosomal subunit.SIMILARITY Belongs to the universal ribosomal protein uS14 family. UniProt P62274 2 EQUAL 56 EQUAL Reactome Database ID Release 78 9754702 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754702 Reactome R-MMU-72367 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72367.1 1 Rps9 RPS9 Q6ZWN5 Reactome DB_ID: 9754715 UniProt:Q6ZWN5 Rps9 Rps9 SUBUNIT Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.SIMILARITY Belongs to the universal ribosomal protein uS4 family. UniProt Q6ZWN5 2 EQUAL 194 EQUAL Reactome Database ID Release 78 9754715 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754715 Reactome R-MMU-72387 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72387.1 1 Rps18 40S ribosomal protein S18 RS18_MOUSE Reactome DB_ID: 9633468 UniProt:P62270 Rps18 Rps18 FUNCTION Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.SIMILARITY Belongs to the universal ribosomal protein uS13 family. UniProt P62270 2 EQUAL 152 EQUAL Reactome Database ID Release 78 9633468 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633468 Reactome R-MMU-9633468 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633468.1 1 Rps21 40S ribosomal protein S21 RS21_MOUSE Reactome DB_ID: 9633595 UniProt:Q9CQR2 Rps21 Rps21 SUBUNIT Component of the 40S small ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eS21 family. UniProt Q9CQR2 1 EQUAL 83 EQUAL Reactome Database ID Release 78 9633595 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633595 Reactome R-MMU-9633595 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633595.1 1 Rps3a RPS3A P97351 Reactome DB_ID: 9754708 UniProt:P97351 Rps3a Rps3a Rps3a1 FUNCTION May play a role during erythropoiesis through regulation of transcription factor DDIT3.SUBUNIT Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Binds with high affinity to IPO4. Interacts with DDIT3.PTM ADP-ribosylated at Tyr-155 by PARP1 in presence of HPF1.SIMILARITY Belongs to the eukaryotic ribosomal protein eS1 family. UniProt P97351 2 EQUAL 264 EQUAL Reactome Database ID Release 78 9754708 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754708 Reactome R-MMU-72373 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72373.1 1 Rps6 40S ribosomal protein S6 RS6_MOUSE Reactome DB_ID: 9633488 UniProt:P62754 Rps6 Rps6 FUNCTION Component of the 40S small ribosomal subunit (By similarity). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (By similarity).PTM Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the pre-initiation complex.PTM Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.SIMILARITY Belongs to the eukaryotic ribosomal protein eS6 family. UniProt P62754 1 EQUAL 249 EQUAL Reactome Database ID Release 78 9633488 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633488 Reactome R-MMU-9633488 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633488.1 1 Rps17 RPS17 P63276 Reactome DB_ID: 9754676 UniProt:P63276 Rps17 Rps17 SIMILARITY Belongs to the eukaryotic ribosomal protein eS17 family. UniProt P63276 2 EQUAL 135 EQUAL Reactome Database ID Release 78 9754676 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754676 Reactome R-MMU-72339 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72339.1 1 Rps19 RPS19 Q9CZX8 Reactome DB_ID: 9754680 UniProt:Q9CZX8 Rps19 Rps19 FUNCTION Required for pre-rRNA processing and maturation of 40S ribosomal subunits.SUBUNIT Interacts with RPS19BP1.SIMILARITY Belongs to the eukaryotic ribosomal protein eS19 family. UniProt Q9CZX8 2 EQUAL 145 EQUAL Reactome Database ID Release 78 9754680 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754680 Reactome R-MMU-72343 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72343.1 1 Rps2 RPS2 P25444 Reactome DB_ID: 9754686 UniProt:P25444 Rps2 Rps2 Llrep3 Rps4 PTM Citrullinated by PADI4 in the Arg/Gly-rich region.PTM Asymmetric arginine dimethylation by PRMT3 occurs at multiple sites in the Arg/Gly-rich region.SIMILARITY Belongs to the universal ribosomal protein uS5 family. UniProt P25444 1 EQUAL 293 EQUAL Reactome Database ID Release 78 9754686 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754686 Reactome R-MMU-72347 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72347.1 1 Rps28 40S ribosomal protein S28 RS28_MOUSE Reactome DB_ID: 9633550 UniProt:P62858 Rps28 Rps28 SUBUNIT Component of the 40S small ribosomal subunit.SIMILARITY Belongs to the eukaryotic ribosomal protein eS28 family. UniProt P62858 1 EQUAL 69 EQUAL Reactome Database ID Release 78 9633550 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633550 Reactome R-MMU-9633550 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633550.1 1 Rps8 40S ribosomal protein S8 RS8_MOUSE Reactome DB_ID: 9633574 UniProt:P62242 Rps8 Rps8 SUBUNIT Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.SIMILARITY Belongs to the eukaryotic ribosomal protein eS8 family. UniProt P62242 2 EQUAL 208 EQUAL Reactome Database ID Release 78 9633574 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633574 Reactome R-MMU-9633574 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633574.1 1 Ghost homologue of 18S rRNA Reactome DB_ID: 9754718 Reactome Database ID Release 78 9754718 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754718 Reactome R-MMU-72391 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72391.1 1 Rps7 40S ribosomal protein S7 RS7_MOUSE Reactome DB_ID: 9633471 UniProt:P62082 Rps7 Rps7 FUNCTION Required for rRNA maturation.SUBUNIT Binds IPO9 with high affinity. Interacts with NEK6. Interacts with DESI2.PTM Phosphorylated by NEK6.PTM Ubiquitinated. Deubiquitinated by DESI2, leading to its stabilization.SIMILARITY Belongs to the eukaryotic ribosomal protein eS7 family. UniProt P62082 1 EQUAL 194 EQUAL Reactome Database ID Release 78 9633471 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633471 Reactome R-MMU-9633471 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633471.1 1 Rps20 40S ribosomal protein S20 RS20_MOUSE Reactome DB_ID: 9633573 UniProt:P60867 Rps20 Rps20 SUBUNIT Component of the 40S small ribosomal subunit.PTM Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.PTM Ufmylated by UFL1.SIMILARITY Belongs to the universal ribosomal protein uS10 family. UniProt P60867 2 EQUAL 119 EQUAL Reactome Database ID Release 78 9633573 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633573 Reactome R-MMU-9633573 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633573.1 1 Converted from EntitySet in Reactome RPS4X,RPS4Y1,RPS4Y2 Reactome DB_ID: 9754741 Rps4x RPS4X P62702 Reactome DB_ID: 9754729 UniProt:P62702 Rps4x Rps4x Rps4 SUBUNIT Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.SIMILARITY Belongs to the eukaryotic ribosomal protein eS4 family. UniProt P62702 2 EQUAL 263 EQUAL Reactome Database ID Release 78 9754729 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754729 Reactome R-MMU-72375 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72375.1 Converted from EntitySet in Reactome Homologues of RPS4Y1 Reactome DB_ID: 9754735 RPS4Y1 Gm15013 V9GWY0 Reactome DB_ID: 9754733 UniProt:V9GWY0 Gm15013 UniProt V9GWY0 2 EQUAL 263 EQUAL Reactome Database ID Release 78 9754733 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754733 Reactome R-MMU-72377 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72377.1 Reactome Database ID Release 78 9754735 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754735 Reactome R-MMU-72377-2 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72377-2.1 Converted from EntitySet in Reactome Homologues of RPS4Y2 Reactome DB_ID: 9754739 Rps4x 40S ribosomal protein S4, X isoform RS4X_MOUSE Reactome DB_ID: 9633559 1 EQUAL 263 EQUAL Reactome Database ID Release 78 9633559 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633559 Reactome R-MMU-9633559 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633559.1 RPS4Y2 Gm15013 V9GWY0 Reactome DB_ID: 9754737 1 EQUAL 263 EQUAL Reactome Database ID Release 78 9754737 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754737 Reactome R-MMU-8868834 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868834.1 Reactome Database ID Release 78 9754739 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754739 Reactome R-MMU-8868834-2 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868834-2.1 Reactome Database ID Release 78 9754741 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754741 Reactome R-MMU-8868832 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8868832.1 1 Rps12 40S ribosomal protein S12 RS12_MOUSE Reactome DB_ID: 9633505 UniProt:P63323 Rps12 Rps12 SIMILARITY Belongs to the eukaryotic ribosomal protein eS12 family. UniProt P63323 2 EQUAL 132 EQUAL Reactome Database ID Release 78 9633505 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9633505 Reactome R-MMU-9633505 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-9633505.1 1 Reactome Database ID Release 78 9754743 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754743 Reactome R-MMU-72392 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72392.1 1 Reactome Database ID Release 78 9755053 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9755053 Reactome R-MMU-72500 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72500.1 1 Ghost homologue of tRNA Reactome DB_ID: 9789335 Reactome Database ID Release 78 9789335 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789335 Reactome R-MMU-141679 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-141679.1 1 Etf1 ETF1 Q8BWY3 Reactome DB_ID: 9789321 UniProt:Q8BWY3 Etf1 UniProt Q8BWY3 2 EQUAL 437 EQUAL Reactome Database ID Release 78 9789321 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789321 Reactome R-MMU-54433 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-54433.1 1 eRF3:GDP Reactome DB_ID: 9789333 Converted from EntitySet in Reactome eRF3 GSPT1,GSPT2 eRF3a,eRF3b Reactome DB_ID: 9789331 Gspt1 GSPT1 Q8R050 Reactome DB_ID: 9789325 UniProt:Q8R050 Gspt1 UniProt Q8R050 1 EQUAL 499 EQUAL Reactome Database ID Release 78 9789325 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789325 Reactome R-MMU-8865759 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8865759.1 Gspt2 GSPT2 Q149F3 Reactome DB_ID: 9789329 UniProt:Q149F3 Gspt2 UniProt Q149F3 1 EQUAL 628 EQUAL Reactome Database ID Release 78 9789329 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789329 Reactome R-MMU-143379 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-143379.1 Reactome Database ID Release 78 9789331 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789331 Reactome R-MMU-8865793 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8865793.1 1 GDP Guanosine 5'-diphosphate Guanosine diphosphate GDP(3-) Reactome DB_ID: 29420 GDP(3-) [ChEBI:58189] GDP(3-) 5'-O-[(phosphonatooxy)phosphinato]guanosine guanosine 5'-diphosphate(3-) GDP guanosine 5'-diphosphate trianion guanosine 5'-diphosphate GDP trianion ChEBI CHEBI:58189 Reactome Database ID Release 78 29420 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=29420 Reactome R-ALL-29420 3 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-29420.3 COMPOUND C00035 additional information MI MI:0361 1 Reactome Database ID Release 78 9789333 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789333 Reactome R-MMU-143378 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-143378.1 1 mRNA Complex with a Premature Termination Codon Preceding an Exon Junction Reactome DB_ID: 9789363 Cap Binding Complex (CBC) Reactome DB_ID: 9756675 Ncbp2 NCBP2 Q9CQ49 Reactome DB_ID: 9756671 UniProt:Q9CQ49 Ncbp2 UniProt Q9CQ49 1 EQUAL 156 EQUAL Reactome Database ID Release 78 9756671 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756671 Reactome R-MMU-162458 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-162458.1 1 Ncbp1 NCBP1 Q3UYV9 Reactome DB_ID: 9756673 UniProt:Q3UYV9 Ncbp1 UniProt Q3UYV9 1 EQUAL 790 EQUAL Reactome Database ID Release 78 9756673 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756673 Reactome R-MMU-162459 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-162459.1 1 Reactome Database ID Release 78 9756675 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9756675 Reactome R-MMU-162460 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-162460.1 1 mRNA with premature termination codon preceding exon junction Reactome DB_ID: 927796 Reactome Database ID Release 78 927796 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=927796 Reactome R-ALL-927796 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-927796.2 ChEBI 33697 1 Pabpc1 PABPC1 P29341 Reactome DB_ID: 9761035 UniProt:P29341 Pabpc1 UniProt P29341 1 EQUAL 636 EQUAL Reactome Database ID Release 78 9761035 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9761035 Reactome R-MMU-156803 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-156803.1 1 Eif4g1 EIF4G1 Q6NZJ6 Reactome DB_ID: 9754840 UniProt:Q6NZJ6 Eif4g1 UniProt Q6NZJ6 1 EQUAL 1599 EQUAL Reactome Database ID Release 78 9754840 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9754840 Reactome R-MMU-72584 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-72584.1 1 Reactome Database ID Release 78 9789363 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789363 Reactome R-MMU-927743 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927743.1 1 Reactome Database ID Release 78 9789376 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789376 Reactome R-MMU-927773 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927773.1 SMG1:UPF1:EJC:Translated mRNP Reactome DB_ID: 9789414 1 1 1 Reactome Database ID Release 78 9789414 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789414 Reactome R-MMU-927767 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927767.1 Reactome Database ID Release 78 9789416 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789416 Reactome R-MMU-927832 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927832.1 The presence of an exon junction complex (EJC) downstream of a termination codon enhances nonsense-mediated decay (NMD) but is not absolutely required for NMD. The EJC is deposited during splicing and remains bound to the mRNA until a ribosome dislodges it during the pioneer round of translation, distinguished by the presence of the cap-binding complex at the 5' end. If translation terminates at least 50-55 nucleotides 5' to an EJC during the pioneer round then termination factors (eRF1 and eRF3) and the EJC recruit UPF1 and other NMD machinery (Lykke-Andersen et al. 2001, Ishigaki et al. 2001, Le Hir et al. 2001, Gehring et al. 2003, Hosoda et al. 2005, Kashima et al. 2006, Singh et al. 2007, Chamieh et al. 2008, Ivanov et al. 2008, Buchwald et al. 2010).<br>A current model for NMD enhanced by the EJC posits recruitment of UPF1, SMG1, SMG8, and SMG9 to eRF3 at the ribosome to form the SURF complex (Kashima et al. 2006, Chang et al. 2007, Isken et al. 2008, Muhlemann et al. 2008, Stalder and Muhlemann 2008, Chamieh et al. 2009, Maquat and Gong 2009, Rebbapragada and Lykke-Andersen 2009, Hwang et al. 2010, Nicholson et al. 2010). UPF1 and SMG1 then interact with components of the EJC, activating phosphorylation of UPF1 by SMG1.<br>The model of the NMD mechanism is inferred from known protein interactions:<br>eRF1 and eRF3 interact with UPF1, the key regulator of NMD which also binds SMG1, UPF2, and UPF3 (UPF3a or UPF3b) to form the SURF complex (Kashima et al.2006, Ivanov et al. 2008, Clerici et al. 2009, Chakrabarti et al. 2011). UPF1 also interacts with CBP80 at the cap of the mRNA (Hwang et al. 2010).<br>SMG8 and SMG9 associate with SMG1 and the SURF complex and modulate the phosphorylation activity of SMG1 (Yamashita et al. 2009).<br>UPF2 and UPF3 are peripheral components of the EJC and thus may link the EJC to the SURF complex (Chamieh et al. 2008). UPF3b binds UPF1 and a composite surface formed by the Y14, MAGOH, and eIF4A3 subunits of the core EJC (Gehring et al. 2003, Kunz et al. 2006, Buchwald et al. 2010). SMG1 also interacts with the EJC (Kashima et al. 2006, Yamashita et al. 2009). UPF3a more weakly activates NMD than does UPF3b (Kunz et al. 2006) and UPF3a levels increase in response to loss of UPF3b (Chan et al. 2009).<br>The binding of UPF1 to translated RNAs may occur in two steps: Binding of the SURF complex to the terminating ribosome followed by transfer of UPF1 and SMG1 to the EJC (Kashima et al. 2006, Hwang et al. 2010).<br>The core EJC (Y14, MAGOH, eIF4A3, and BTZ) can activate NMD without UPF2, however RNPS1, another EJC subunit, requires UPF2 to activate NMD (Gehring et al. 2005). RNAs show differential dependence on RNPS1-activated NMD (Gehring et al. 2005). Also, NMD of some transcripts requires EJC component eIF4A3 but not UPF3b (Chan et al. 2007) therefore there may be more than one route to activating NMD via the EJC. 11532962 Pubmed 2001 The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay Le Hir, H Gatfield, D Izaurralde, E Moore, MJ EMBO J 20:4987-97 18066079 Pubmed 2008 NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity Chamieh, H Ballut, L Bonneau, F Le Hir, H Nat Struct Mol Biol 15:85-93 18657639 Pubmed 2008 Recognition and elimination of nonsense mRNA Muhlemann, Oliver Eberle, AB Stalder, L Zamudio Orozco, R Biochim Biophys Acta 1779:538-49 18256688 Pubmed 2008 Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways Ivanov, PV Gehring, NH Kunz, JB Hentze, MW Kulozik, AE EMBO J 27:736-47 17363904 Pubmed 2007 An alternative branch of the nonsense-mediated decay pathway Chan, WK Huang, L Gudikote, JP Chang, YF Imam, JS MacLean JA, 2nd Wilkinson, MF EMBO J 26:1820-30 12718880 Pubmed 2003 Y14 and hUpf3b form an NMD-activating complex Gehring, NH Neu-Yilik, Gabriele Schell, T Hentze, MW Kulozik, AE Mol Cell 11:939-49 21419344 Pubmed 2011 Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2 Chakrabarti, S Jayachandran, U Bonneau, F Fiorini, F Basquin, C Domcke, S Le Hir, H Conti, E Mol Cell 41:693-703 19478851 Pubmed 2009 The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay Gehring, NH Lamprinaki, S Hentze, MW Kulozik, AE PLoS Biol 7:e1000120 16209946 Pubmed 2005 Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements Gehring, NH Kunz, JB Neu-Yilik, Gabriele Breit, S Viegas, MH Hentze, MW Kulozik, AE Mol Cell 20:65-75 18423202 Pubmed 2008 Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay Isken, O Kim, YK Hosoda, N Mayeur, GL Hershey, John W Maquat, LE Cell 133:314-27 14973490 Pubmed 2004 An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay Palacios, IM Gatfield, D St Johnston, D Izaurralde, E Nature 427:753-7 20691628 Pubmed 2010 UPF1 association with the cap-binding protein, CBP80, promotes nonsense-mediated mRNA decay at two distinct steps Hwang, J Sato, H Tang, Y Matsuda, D Maquat, LE Mol Cell 39:396-409 17352659 Pubmed 2007 The nonsense-mediated decay RNA surveillance pathway Chang, YF Imam, JS Wilkinson, MF Annu Rev Biochem 76:51-74 16186820 Pubmed 2005 CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells Hosoda, N Kim, YK Lejeune, F Maquat, LE Nat Struct Mol Biol 12:893-901 17803942 Pubmed 2007 Communication with the exon-junction complex and activation of nonsense-mediated decay by human Upf proteins occur in the cytoplasm Singh, G Jakob, S Kleedehn, MG Lykke-Andersen, J Mol Cell 27:780-92 16452507 Pubmed 2006 Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay Kashima, I Yamashita, A Izumi, N Kataoka, N Morishita, R Hoshino, S Ohno, M Dreyfuss, G Ohno, S Genes Dev 20:355-67 19503078 Pubmed 2009 A UPF3-mediated regulatory switch that maintains RNA surveillance Chan, WK Bhalla, AD Le Hir, H Nguyen, LS Huang, L Gécz, J Wilkinson, MF Nat Struct Mol Biol 16:747-53 19359157 Pubmed 2009 Execution of nonsense-mediated mRNA decay: what defines a substrate? Rebbapragada, I Lykke-Andersen, J Curr Opin Cell Biol 21:394-402 20479275 Pubmed 2010 Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex Buchwald, G Ebert, J Basquin, C Sauliere, J Jayachandran, U Bono, F Le Hir, H Conti, E Proc Natl Acad Sci U S A 107:10050-5 19859661 Pubmed 2010 Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factors Nicholson, P Yepiskoposyan, H Metze, S Zamudio Orozco, R Kleinschmidt, N Muhlemann, Oliver Cell Mol Life Sci 67:677-700 19417104 Pubmed 2009 SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay Yamashita, A Izumi, N Kashima, I Ohnishi, T Saari, B Katsuhata, Y Muramatsu, R Morita, T Iwamatsu, A Hachiya, T Kurata, R Hirano, H Anderson, P Ohno, S Genes Dev 23:1091-105 15034551 Pubmed 2004 eIF4AIII binds spliced mRNA in the exon junction complex and is essential for nonsense-mediated decay Shibuya, T Tange, TØ Sonenberg, Nahum Moore, MJ Nat Struct Mol Biol 11:346-51 11546874 Pubmed 2001 Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1 Lykke-Andersen, J Shu, MD Steitz, JA Science 293:1836-9 18524595 Pubmed 2008 The meaning of nonsense Stalder, L Muhlemann, Oliver Trends Cell Biol 18:315-21 16601204 Pubmed 2006 Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation Kunz, JB Neu-Yilik, Gabriele Hentze, MW Kulozik, AE Gehring, NH RNA 12:1015-22 19909264 Pubmed 2009 Gene expression networks: competing mRNA decay pathways in mammalian cells Maquat, LE Gong, C Biochem Soc Trans 37:1287-92 19556969 Pubmed 2009 Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2 Clerici, M Mourão, A Gutsche, I Gehring, NH Hentze, MW Kulozik, A Kadlec, J Sattler, M Cusack, S EMBO J 28:2293-306 11551508 Pubmed 2001 Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20 Ishigaki, Y Li, X Serin, G Maquat, LE Cell 106:607-17 inferred from electronic annotation EVIDENCE CODE ECO:0000203 LEFT-TO-RIGHT 2.7.11.13 SMG1 phosphorylates UPF1 (enhanced by Exon Junction Complex) This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> ATP Adenosine 5'-triphosphate ATP(4-) Reactome DB_ID: 113592 ATP(4-) [ChEBI:30616] ATP(4-) ATP atp Adenosine 5'-triphosphate ChEBI CHEBI:30616 Reactome Database ID Release 78 113592 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=113592 Reactome R-ALL-113592 5 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-113592.5 COMPOUND C00002 4 SMG1:Phosphorylated UPF1:EJC:Translated mRNP Reactome DB_ID: 9789398 Q9EPU0 phospho-Upf1 p-4S-UPF1 Reactome DB_ID: 9789396 1089 EQUAL O-phospho-L-serine MOD MOD:00046 1107 EQUAL 1084 EQUAL 1127 EQUAL 1 EQUAL 1129 EQUAL Reactome Database ID Release 78 9789396 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789396 Reactome R-MMU-927856 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927856.1 1 1 1 Reactome Database ID Release 78 9789398 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789398 Reactome R-MMU-927890 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927890.1 ADP Adenosine 5'-diphosphate ADP(3-) Reactome DB_ID: 29370 ADP(3-) [ChEBI:456216] ADP(3-) ADP 5&apos;-O-[(phosphonatooxy)phosphinato]adenosine ADP trianion ChEBI CHEBI:456216 Reactome Database ID Release 78 29370 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=29370 Reactome R-ALL-29370 5 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-29370.5 COMPOUND C00008 4 ACTIVATION activeUnit: #Protein2 GENE ONTOLOGY GO:0004697 gene ontology term for cellular function MI MI:0355 Same Catalyst Activity Reactome Database ID Release 78 9789420 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789420 Reactome Database ID Release 78 9789422 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789422 Reactome R-MMU-927889 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927889.1 SMG1 phosphorylates UPF1 in vitro and in vivo (Denning et al. 2001, Yamashita et al. 2001, Kashima et al. 2006). Serines 1073, 1078, 1096, and 1116 in isoform 2 (Serines 1084, 1089, 1107, 1127 in isoform 1) are phosphorylated in vitro and phosphorylation at serines 1078 and 1096 has been confirmed in vivo (Yamashita et al. 2001, Ohnishi et al. 2003, Kashima et al. 2006). UPF1 also contains additional serine and threonine residues that could be phosphorylated. SMG8 and SMG9 associate with SMG1 and regulate the kinase activity of SMG1 (Yamashita et al. 2009). The phosphorylation reaction is rate-limiting in nonsense-mediated decay and is therefore regarded as a licensing step (reviewed in Rebbapragada and Lykke-Andersen 2009). Phosphorylation is enhanced by the exon junction complex, which can interact with UPF1 via UPF2 and/or UPF3 (Kashima et al. 2006, Ivanov et al. 2008) or via Y14:Magoh (Ivanov et al. 2008). SMG8 and SMG9 bind SMG1 and regulate its kinase activity (Yamashita et al. 2009, Fernandez et al. 2011). 11331269 Pubmed 2001 Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein Denning, G Jamieson, L Maquat, LE Thompson, EA Fields, AP J Biol Chem 276:22709-14 14636577 Pubmed 2003 Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7 Ohnishi, T Yamashita, A Kashima, I Schell, T Anders, KR Grimson, A Hachiya, T Hentze, MW Anderson, P Ohno, S Mol Cell 12:1187-200 20817927 Pubmed 2011 Characterization of SMG-9, an essential component of the nonsense-mediated mRNA decay SMG1C complex Fernández, IS Yamashita, A Arias-Palomo, E Bamba, Y Bartolomé, RA Canales, MA Teixidó, J Ohno, S Llorca, O Nucleic Acids Res 39:347-58 11544179 Pubmed 2001 Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay Yamashita, A Ohnishi, T Kashima, I Taya, Y Ohno, S Genes Dev 15:2215-28 LEFT-TO-RIGHT p-4S-UPF1 recruits SMG5, SMG7, SMG6, PNRC2, DCP1A, and PP2A This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> PP2A (Aalpha:B55alpha:Calpha) Reactome DB_ID: 9789349 Ppp2ca PPP2CA P63330 Reactome DB_ID: 9760693 UniProt:P63330 Ppp2ca UniProt P63330 1 EQUAL 309 EQUAL Reactome Database ID Release 78 9760693 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9760693 Reactome R-MMU-165971 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-165971.1 1 Q6P1F6 Ppp2r2a PPP2R2A Reactome DB_ID: 9789347 UniProt:Q6P1F6 Ppp2r2a UniProt Q6P1F6 2 EQUAL 447 EQUAL Reactome Database ID Release 78 9789347 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789347 Reactome R-MMU-377253 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-377253.1 1 PP2A A subunit Reactome DB_ID: 206067 UniProt:Q76MZ3 Ppp2r1a Ppp2r1a FUNCTION The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit (PubMed:10100624). Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (By similarity). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (By similarity).SUBUNIT PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity). Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphorylation sites (PubMed:22417654). Interacts with IPO9 (By similarity). Interacts with TP53 and SGO1 (By similarity). Interacts with PLA2G16; this interaction might decrease PP2A activity (By similarity). Interacts with CTTNBP2NL (By similarity). Interacts with GNA12; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT (By similarity).Interacts with CIP2A; this interaction stabilizes CIP2A (By similarity). Interacts with PABIR1/FAM122A (By similarity). Interacts with ADCY8; antagonizes interaction between ADCY8 and calmodulin (PubMed:16258073). Interacts with CRTC3 (when phosphorylated at 'Ser-391') (PubMed:30611118). Interacts with SPRY2 (By similarity).DOMAIN Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.SIMILARITY Belongs to the phosphatase 2A regulatory subunit A family. UniProt Q76MZ3 2 EQUAL 589 EQUAL Reactome Database ID Release 78 206067 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=206067 Reactome R-MMU-206067 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-206067.1 1 Reactome Database ID Release 78 9789349 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789349 Reactome R-MMU-377182 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-377182.1 Smg5 SMG5 Q6ZPY2 Reactome DB_ID: 9789353 UniProt:Q6ZPY2 Smg5 UniProt Q6ZPY2 1 EQUAL 1016 EQUAL Reactome Database ID Release 78 9789353 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789353 Reactome R-MMU-927867 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927867.1 Smg7 SMG7 Q5RJH6 Reactome DB_ID: 9789357 UniProt:Q5RJH6 Smg7 UniProt Q5RJH6 1 EQUAL 1137 EQUAL Reactome Database ID Release 78 9789357 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789357 Reactome R-MMU-927838 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927838.1 Smg6 SMG6 P61406 Reactome DB_ID: 9789361 UniProt:P61406 Smg6 UniProt P61406 1 EQUAL 1419 EQUAL Reactome Database ID Release 78 9789361 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789361 Reactome R-MMU-927851 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927851.1 Pnrc2 PNRC2 Q9CR73 Reactome DB_ID: 9789402 UniProt:Q9CR73 Pnrc2 UniProt Q9CR73 1 EQUAL 139 EQUAL Reactome Database ID Release 78 9789402 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789402 Reactome R-MMU-8879146 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-8879146.1 Dcp1a DCP1A Q91YD3 Reactome DB_ID: 9783210 UniProt:Q91YD3 Dcp1a UniProt Q91YD3 1 EQUAL 582 EQUAL Reactome Database ID Release 78 9783210 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9783210 Reactome R-MMU-430043 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-430043.1 Phosphorylated UPF1:SMG5:SMG7:SMG6:PP2A:Translated mRNP Reactome DB_ID: 9789404 1 1 1 1 1 1 1 Reactome Database ID Release 78 9789404 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789404 Reactome R-MMU-927854 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927854.1 Reactome Database ID Release 78 9789406 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789406 Reactome R-MMU-927813 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927813.1 SMG6, SMG5 and SMG7 contain 14-3-3 domains which are believed to bind phosphorylated SQ motifs in UPF1 (Chiu et al. 2003, Ohnishi et al. 2003, Unterholzner and Izaurralde 2004, Fukuhara et al. 2005, Durand et al. 2007). SMG7 has been shown to bind UPF1 directly, target UPF1 for dephosphorylation by PP2A, and recruit enzymes that degrade RNA (Ohnishi et al. 2003, Unterholzner and Izaurralde 2004, Fukuhara et al. 2005). UPF3AS (the small isoform of UPF3A) also associates with the complex (Ohnishi et al. 2003). SMG6 is an endoribonuclease that cleaves the mRNA bound by UPF1 and also recruits phosphatase PP2A to dephosphorylate UPF1 (Chiu et al. 2003, Glavan et al. 2006, Eberle et al. 2009). PNRC2 binds both phospo-UPF1 and the decapping enzyme DCP1A, thereby facilitating decapping of the mRNA (Cho et al. 2009, Lai et al. 2012, Cho et al. 2013).<br>Though immunofluorescence in vivo indicates that SMG5 and SMG7 exist in separate complexes from SMG6 (Unterholzner and Izaurralde 2004) immunoprecipitation shows that SMG6 is present in complexes that also contain SMG5, SMG7, UPF1, UPF2, Y14, Magoh, and PABP (Kashima et al. 2010). SMG5, SMG6, and SMG7 are therefore represented here together in the same RNP complex. It is possible that some complexes contain only SMG6 or SMG5:SMG7 (reviewed in Nicholson et al. 2010, Muhlemann and Lykke-Andersen 2010). Note that "Smg5/7a" in Chiu et al. 2003 actually refers to SMG6.<br>Phosphorylated UPF1 also inhibits translation initiation by inhibiting conversion of 40S:tRNAmet:mRNA to 80S:tRNAmet:mRNA complexes (Isken et al. 2008) 19060897 Pubmed 2009 SMG6 promotes endonucleolytic cleavage of nonsense mRNA in human cells Eberle, AB Lykke-Andersen, S Muhlemann, Oliver Jensen, TH Nat Struct Mol Biol 16:49-55 20023408 Pubmed 2010 How and where are nonsense mRNAs degraded in mammalian cells? Muhlemann, Oliver Lykke-Andersen, J RNA Biol 7:28-32 23234702 Pubmed 2013 SMG5-PNRC2 is functionally dominant compared with SMG5-SMG7 in mammalian nonsense-mediated mRNA decay Cho, Hana Han, Sisu Choe, Junho Park, Seung Gu Choi, Sun Shim Kim, Yoon Ki Nucleic Acids Res. 41:1319-28 23085078 Pubmed 2012 Structural basis of the PNRC2-mediated link between mrna surveillance and decapping Lai, Tingfeng Cho, Hana Liu, Zhou Bowler, Matthew W Piao, Shunfu Parker, Roy Kim, Yoon Ki Song, Haiwei Structure 20:2025-37 17053788 Pubmed 2006 Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillance complex Glavan, F Behm-Ansmant, I Izaurralde, E Conti, E EMBO J 25:5117-25 15721257 Pubmed 2005 SMG7 is a 14-3-3-like adaptor in the nonsense-mediated mRNA decay pathway Fukuhara, N Ebert, J Unterholzner, L Lindner, D Izaurralde, E Conti, E Mol Cell 17:537-47 15546618 Pubmed 2004 SMG7 acts as a molecular link between mRNA surveillance and mRNA decay Unterholzner, L Izaurralde, E Mol Cell 16:587-96 20930030 Pubmed 2010 SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay Kashima, I Jonas, S Jayachandran, U Buchwald, G Conti, E Lupas, AN Izaurralde, E Genes Dev 24:2440-50 17893241 Pubmed 2007 Inhibition of nonsense-mediated mRNA decay (NMD) by a new chemical molecule reveals the dynamic of NMD factors in P-bodies Durand, S Cougot, N Mahuteau-Betzer, F Nguyen, CH Grierson, DS Bertrand, E Tazi, J Lejeune, F J Cell Biol 178:1145-60 12554878 Pubmed 2003 Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1 Chiu, SY Serin, G Ohara, O Maquat, LE RNA 9:77-87 19150429 Pubmed 2009 Human proline-rich nuclear receptor coregulatory protein 2 mediates an interaction between mRNA surveillance machinery and decapping complex Cho, Hana Kim, Kyoung Mi Kim, Yoon Ki Mol. Cell 33:75-86 LEFT-TO-RIGHT SMG6 hydrolyzes mRNA with premature termination codon This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> mRNA Cleaved by SMG6 Reactome DB_ID: 9789408 1 1 1 1 1 1 1 3' Fragment of Cleaved mRNA Reactome DB_ID: 927738 Reactome Database ID Release 78 927738 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=927738 Reactome R-ALL-927738 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-927738.2 ChEBI 33697 1 1 5' Fragment of Cleaved mRNA Reactome DB_ID: 927835 Reactome Database ID Release 78 927835 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=927835 Reactome R-ALL-927835 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-927835.2 ChEBI 33697 1 Reactome Database ID Release 78 9789408 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789408 Reactome R-MMU-927845 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927845.1 ACTIVATION activeUnit: #Protein125 GENE ONTOLOGY GO:0004521 Reactome Database ID Release 78 9789417 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789417 Reactome Database ID Release 78 9789419 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789419 Reactome R-MMU-927836 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927836.1 SMG6 is an endoribonuclease which cleaves the mRNA bound by UPF1 near the premature termination codon (Glavan et al. 2006, Eberle et al. 2009). 18974281 Pubmed 2008 SMG6 is the catalytic endonuclease that cleaves mRNAs containing nonsense codons in metazoan Huntzinger, E Kashima, I Fauser, M Saulière, J Izaurralde, E RNA 14:2609-17 LEFT-TO-RIGHT Decay of mRNA in SMG6:SMG5:SMG7:mRNA complex This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a> Reactome Database ID Release 78 9789410 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789410 Reactome R-MMU-927830 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-927830.1 SMG6 endonucleolytically cleaves an mRNA it is believed that the resulting fragments are degraded by exonucleases, possibly XRN1, a 5'-to-3' nuclease, and the exosome complex, a 3'-to-5' nuclease (Huntzinger et al. 2008, Eberle et al. 2009). Inhibition of XRN1 is observed to cause accumulation of SMG6-cleaved intermediates therefore XRN1 is postulated to act downstream of SMG6 (Huntzinger et al. 2008).<br>In general, during Nonsense-Mediated Decay mRNAs are observed to be deadenlyated (implicating the PAN2 complex, PARN complex, and CCR4 complex), decapped (implicating the DCP1:DCP2 complex), and exoribonucleolytically digested (implicating the XRN1 5'-to-3' exonuclease and exosome 3'-to-5' exonuclease) (Lykke-Andersen 2002, Chen et al. 2003, Lejeune et al. 2003, Couttet and Grange 2004, Unterholzner and Izaurralde 2004, Yamashita et al. 2005). UPF1 is observed to associate with the decapping enzymes DCP1a and DCP2, however the specific decay reactions that occur after SMG6, SMG5 and SMG7 have associated with an mRNA are unknown (Lykke-Andersen et al. 2002). Likewise, SMG6 may be present in complexes separate from SMG5 and SMG7 and these complexes may have different routes of decay (reviewed in Nicholson et al. 2010, Muhlemann and Lykke-Andersen 2010).<br>ATPase activity of UPF1 is necessary for NMD and may reflect ATP-dependent helicase activity that disassembles the mRNA-protein complex (Franks et al. 2010). UPF1 must be dephosphorylated by PP2A for NMD to continue (Ohnishi et al. 2003, Chiu et al. 2003). Presumably the dephosphoryation recycles UPF1 for interaction with other mRNA complexes. 21145460 Pubmed 2010 Upf1 ATPase-dependent mRNP disassembly is required for completion of nonsense- mediated mRNA decay Franks, TM Singh, G Lykke-Andersen, J Cell 143:938-50 16284618 Pubmed 2005 Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover Yamashita, A Chang, TC Yamashita, Y Zhu, W Zhong, Z Chen, CY Shyu, AB Nat Struct Mol Biol 12:1054-63 12832468 Pubmed 2003 Rapid deadenylation triggered by a nonsense codon precedes decay of the RNA body in a mammalian cytoplasmic nonsense-mediated decay pathway Chen, CY Shyu, AB Mol Cell Biol 23:4805-13 12417715 Pubmed 2002 Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay Lykke-Andersen, J Mol Cell Biol 22:8114-21 14742663 Pubmed 2004 Premature termination codons enhance mRNA decapping in human cells Couttet, P Grange, T Nucleic Acids Res 32:488-94 14527413 Pubmed 2003 Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities Lejeune, F Li, X Maquat, LE Mol Cell 12:675-87 ACTIVATION Reactome Database ID Release 78 9789412 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789412 ACTIVATION Reactome Database ID Release 78 9789411 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9789411 Reactome Database ID Release 78 9862006 Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=9862006 Reactome R-MMU-975957 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=R-MMU-975957.1 During normal translation termination eRF3 associates with the ribosome and then interacts with PABP bound to the polyadenylate tail of the mRNA to release the ribosome and allow a new round of translation to commence. Nonsense-mediated decay (NMD) is triggered if eRF3 at the ribosome interacts with UPF1, which may compete with PABP (reviewed in Isken and Maquat 2007, Chang et al. 2007, Behm-Ansmant et al. 2007, Rebbapragada and Lykke-Andersen 2009, Bhuvanagiri et al. 2010, Nicholson et al. 2010, Durand and Lykke-Andersen 2011). An exon junction located 50-55 nt downstream of a termination codon is observed to enhance NMD.<br> Exon-junction complexes (EJCs) are deposited on the mRNA during splicing in the nucleus, remain on mRNAs after transport to the cytosol, and are dislodged by the ribosome as it progresses along the mRNA during the pioneer round of translation (Gehring et al. 2009). EJCs contain the core factors eIF4A-III, Magoh-Y14, and CASC3 as well as the peripheral factors RNPS1, UPF2, and UPF3. UPF2 and UPF3 recruit UPF1 to eRF3 at the terminating ribosome. Thus an EJC downstream of a termination codon will not have been dislodged during translation and will recruit UPF1, triggering NMD.<br>UPF1 is believed to form a complex containing SMG1, SMG8, and SMG9. In the key regulatory step of NMD SMG1 phosphorylates UPF1. The phosphorylated UPF1 then recruits either SMG6 or SMG5 and SMG7. SMG6 is itself an endoribonuclease that cleaves the mRNA. SMG5 and SMG7 do not have endoribonuclease activty, but are thought to recruit ribonucleases. Nonsense-mediated decay has been observed to involve deadenlyation, decapping, and both 5' to 3' and 3' to 5' exonuclease activities, but the exact degradative pathways taken by a given mRNA are not yet known.<br>UPF1 also plays roles in Staufen-mediated decay, histone mRNA decay, telomere maintenance, genome integrity, and may play a role in normal termination of translation. 17531985 Pubmed 2007 mRNA quality control: an ancient machinery recognizes and degrades mRNAs with nonsense codons Behm-Ansmant, I Kashima, I Rehwinkel, J Saulière, J Wittkopp, N Izaurralde, E FEBS Lett 581:2845-53 21496649 Pubmed 2011 SnapShot: Nonsense-Mediated mRNA Decay Durand, S Lykke-Andersen, J Cell 145:324-324.e2 17671086 Pubmed 2007 Quality control of eukaryotic mRNA: safeguarding cells from abnormal mRNA function Isken, O Maquat, LE Genes Dev 21:1833-56 20795950 Pubmed 2010 NMD: RNA biology meets human genetic medicine Bhuvanagiri, M Schlitter, AM Hentze, MW Kulozik, AE Biochem J 430:365-77 19410547 Pubmed 2009 Disassembly of exon junction complexes by PYM Gehring, NH Lamprinaki, S Kulozik, AE Hentze, MW Cell 137:536-48