Reactome: A Curated Pathway Database
Results 1 to 10 of 21
Pathways (4) Reactions (8) Proteins (1) Others (8)
Protein: UniProt:P35637 FUS (Homo sapiens)
Last changed: 2015-03-10 08:59:22

Pathway: Gene Expression (Homo sapiens)
Gene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are processed. Most annotation is centered on the generation of messenger RNAs (mRNAs) by regulated RNA polymerase II (PolII) transcription, although the activities of PolI and PolIII are also covered briefly, as are
Last changed: 2015-03-06 23:15:47

Pathway: Processing of Capped Intron-Containing Pre-mRNA (Homo sapiens)
Co-transcriptional pre-mRNA splicing is not obligatory. Pre-mRNA splicing begins co-transcriptionally and often continues post-transcriptionally. Human genes contain an average of nine introns per gene, which cannot serve as splicing substrates until both 5' and 3' ends of each intron are synthesized. Thus the time that it takes for pol II to synthesize each intron defines a minimal time and distance a
Last changed: 2015-03-06 23:15:47

Pathway: mRNA Splicing (Homo sapiens)
The process in which excision of introns from the primary transcript of messenger RNA (mRNA) is followed by ligation of the two exon termini exposed by removal of each intron, is called mRNA splicing. Most of the mRNA is spliced by the major pathway, involving the U1, U2, U4, U5 and U6 snRNPs. A minor fraction, about 1 %, of the mRNAs are spliced via the U12 dependent pathway
Last changed: 2015-03-06 23:15:47

Pathway: mRNA Splicing - Major Pathway (Homo sapiens)
The splicing of pre-mRNA occurs within a large, very dynamic complex, designated the 'spliceosome'. The 50-60S spliceosomes are estimated to be 40-60 nm in diameter, and have molecular weights in the range of 3-5 million kDa. Small nuclear RNAs (snRNAs) U1, U2, U4, U5, and U6, are some of the best characterized components of spliceosomes, and are known to play key roles not only in spliceosomal assembl
Last changed: 2015-03-06 23:15:47

Reaction: Lariat Formation and 5'-Splice Site Cleavage (Homo sapiens)
In the first catalytic step of mRNA splicing, the 2' OH group of the bulged A at the branch site performs a nucleophilic attack on the 5' splice site phosphodiester bond, resulting in cleavage of the bond between the 5' exon and the 5' end of the intron, and formation of a new bond between the 5' end of the intron and the branch site A. This results in a lariat-shaped intermediate, with the intron sti
Last changed: 2015-03-06 23:15:47

Reaction: Formation of the Spliceosomal B Complex (Homo sapiens)
The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex
Last changed: 2015-03-06 23:15:47

Reaction: Formation of the Spliceosomal A Complex (Homo sapiens)
The A complex is the first ATP-dependent complex in spliceosome assembly. U2AF recruits the U2 snRNP to bind to the branch site in the E complex in an ATP-dependent fashion, to form the A complex. The U2 snRNA base-pairs with the branch site, causing the branch-site adenosine to bulge out, which later positions it for nucleophilic attack at the 5' splice site. The A complex serves as a substrate for fo
Last changed: 2014-05-23 07:46:57

Reaction: Cleavage at the 3'-Splice Site and Exon Ligation (Homo sapiens)
The second step of the splicing reaction results in cleavage of the transcript at the 3'splice site, and results in ligation of the two exons and excision of the intron
Last changed: 2015-03-05 21:12:39

Reaction: Formation of the active Spliceosomal C complex (Homo sapiens)
The active C complex is formed due to a conformational change in the intermediate C complex. After formation of the active C complex, the splicing reactions occur very rapidly
Last changed: 2015-03-06 23:15:47

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