Reactome: A Curated Pathway Database

Insertion of correct bases opposite the lesion by POLH

Stable Identifier
R-HSA-110317
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

DNA polymerase eta (POLH) correctly incorporates two adenine deoxyribonucleotides (dAMPs) opposite a TT-CPD (thymine-thymine cyclobutane pyrimidine dimer) lesion. POLH can bypass other types of lesions, such as AP sites and cisplatin-induced intrastrand cross-linked gunanines, preferentially incorporating dAMPs and dGMPs opposite the lesion. While POLH is accurate in translesion synthesis (TLS) across thymine dimers, POLH has a low fidelity in TLS across other DNA damage types and when copying undamaged DNA. One of the protective mechanisms against POLH-induced mutagenesis may be that POLH cannot continue chain elongation after an incorrect nucleotide is incorporated (Matsuda et al. 2000, Masutani et al. 2000).

Literature References
Participants
Participant Of
This entity is regulated by:
Title Physical Entity Activity
DNA-directed DNA polymerase activity of POLH:MonoUb:K164-PCNA:RPA:RFC:TT-CPD-DNA Template [nucleoplasm] POLH:MonoUb:K164-PCNA:RPA:RFC:TT-CPD-DNA Template [nucleoplasm] DNA-directed DNA polymerase activity (0003887)
Orthologous Events