Reactome | Addition of nucleotides between position +11 and +30

Addition of nucleotides between position +11 and +30

Stable Identifier

R-HSA-111264

Type

Reaction

Species

Homo sapiens

Compartment

nucleoplasm

Locations in the PathwayBrowser

Summation

RNA polymerase II transcription complexes are susceptible to transcriptional stalling and arrest, when extending nascent transcripts to 30-nt. This susceptibility depends on presence on down-stream DNA, the particular DNA-sequence of the template and presence of transcription factors. Transcription factor TFIIH remains associated to the RNA pol II elongation complex until position +30. At this stage transcription elongation factor TFIIS can rescue stalled transcription elongation complexes. The transcription bubble varies between 13- and 22-nt in size.

Literature References

11433015	Analysis of the open region of RNA polymerase II transcription complexes in the early phase of elongation.	Nucleic Acids Res	2001
11486021	Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence.	Mol Cell Biol	2001
9353262	Promoter escape by RNA polymerase II. Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter.	J Biol Chem	1997
7601352	Recycling of the general transcription factors during RNA polymerase II transcription.	Genes Dev	1995

Participants

input

output

Participant Of

hasEvent

RNA Polymerase II Promoter Escape

This entity is regulated by:

Title	Physical Entity	Activity

DNA-directed RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

DNA-directed RNA polymerase activity (0003899)

Orthologous Events