Reactome: A Curated Pathway Database

Addition of nucleotides between position +11 and +30

Stable Identifier
R-HSA-111264
Type
Reaction
Species
Homo sapiens
Compartment
nucleoplasm
Locations in the PathwayBrowser
Summation

RNA polymerase II transcription complexes are susceptible to transcriptional stalling and arrest, when extending nascent transcripts to 30-nt. This susceptibility depends on presence on down-stream DNA, the particular DNA-sequence of the template and presence of transcription factors. Transcription factor TFIIH remains associated to the RNA pol II elongation complex until position +30. At this stage transcription elongation factor TFIIS can rescue stalled transcription elongation complexes. The transcription bubble varies between 13- and 22-nt in size.

Literature References
PubMed ID Title Journal Year
11486021 Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence. Mol Cell Biol 2001
7601352 Recycling of the general transcription factors during RNA polymerase II transcription. Genes Dev 1995
9353262 Promoter escape by RNA polymerase II. Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter. J Biol Chem 1997
11433015 Analysis of the open region of RNA polymerase II transcription complexes in the early phase of elongation. Nucleic Acids Res 2001
Participants
input
output
Participant Of
hasEvent
This entity is regulated by:
Title Physical Entity Activity
DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] DNA-directed 5'-3' RNA polymerase activity (0003899)
Orthologous Events
 
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