Reactome | Addition of nucleotides between position +11 and +30

Reactome will be unavailable due to host maintenance from Friday, April 27 at 6pm to Sunday, April 29 at 11:55pm.

During the outage, you may wish to use our mirror site at reactome.ncpsb.org.

Addition of nucleotides between position +11 and +30

Stable Identifier

R-HSA-111264

Type

Reaction

Species

Homo sapiens

Compartment

nucleoplasm

Locations in the PathwayBrowser

Expand all

Summation

RNA polymerase II transcription complexes are susceptible to transcriptional stalling and arrest, when extending nascent transcripts to 30-nt. This susceptibility depends on presence on down-stream DNA, the particular DNA-sequence of the template and presence of transcription factors. Transcription factor TFIIH remains associated to the RNA pol II elongation complex until position +30. At this stage transcription elongation factor TFIIS can rescue stalled transcription elongation complexes. The transcription bubble varies between 13- and 22-nt in size.

Literature References

PubMed ID	Title	Journal	Year
11486021	Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence.	Mol Cell Biol	2001
7601352	Recycling of the general transcription factors during RNA polymerase II transcription.	Genes Dev	1995
9353262	Promoter escape by RNA polymerase II. Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter.	J Biol Chem	1997
11433015	Analysis of the open region of RNA polymerase II transcription complexes in the early phase of elongation.	Nucleic Acids Res	2001