Addition of nucleotides leads to transcript elongation

Stable Identifier
R-HSA-112385
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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High-resolution structures of free, catalytically active yeast Pol II and of an elongating form reveal that Pol II elongation complex includes features like:
- RNA-DNA hybrid, an unwound template ahead of 3'-OH terminus of growing transcript and an exit groove at the base of the CTD, possibly for dynamic interaction of processing and transcriptional factors.
- a cleft or channel created by Rpb1 and Rpb2 subunits to accommodate DNA template, extending to Mg2+ ion located deep in the enzyme core
-a 50 kDa "clamp" with open confirmation in free polymerase, allowing entry of DNA strands but closed in the processive elongation phase.
The clamp is composed of portions of Rpb1,Rpb2 and Rpb3 , five loops or "switches" that change from unfolded to well-folded structures stabilizing the elongation complex, and a long "bridging helix" that emanates from Rpb1 subunit, crossing near the Mg2+ ion. The bridging helix is thought to "bend" to push on the base pair at the 3'-end of RNA-DNA hybrid like a ratchet, translocating Pol II along the DNA (Cramer et al.,2001; Gnatt et al.,2001).In addition to its dynamic biochemical potential, Pol II possess a repertoire of functions to serve as a critical platform of recruiting and coordinating the actions of a host of additional enzyme and proteins involved in various pathways.

Literature References
PubMed ID Title Journal Year
11313499 Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution.

Gnatt, AL, Cramer, P, Fu, J, Bushnell, DA, Kornberg, RD

Science 2001
11313498 Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution.

Cramer, P, Bushnell, DA, Kornberg, RD

Science 2001
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