Reactome: A Curated Pathway Database

G alpha (s) auto-inactivates by hydrolysing GTP to GDP

Stable Identifier
Homo sapiens
Locations in the PathwayBrowser

When a ligand activates a G protein-coupled receptor, it induces a conformational change in the receptor (a change in shape) that allows the receptor to function as a guanine nucleotide exchange factor (GEF), stimulating the exchange of GDP for GTP on the G alpha subunit. In the traditional view of heterotrimeric protein activation, this exchange triggers the dissociation of the now active G alpha subunit from the beta:gamma dimer, initiating downstream signalling events. The G alpha subunit has intrinsic GTPase activity and will eventually hydrolyze the attached GTP to GDP, allowing reassociation with G beta:gamma. Additional GTPase-activating proteins (GAPs) stimulate the GTPase activity of G alpha, leading to more rapid termination of the transduced signal. In some cases the downstream effector may have GAP activity, helping to deactivate the pathway. This is the case for phospholipase C beta, which possesses GAP activity within its C-terminal region (Kleuss et al. 1994).

Literature References
Participant Of
This entity is regulated by:
Title Physical Entity Activity
GTPase activity of G-protein alpha (s):GTP [plasma membrane] G-protein alpha (s):GTP [plasma membrane] GTPase activity (0003924)
Orthologous Events