RNA polymerase II transcription complexes are susceptible to transcriptional stalling and arrest, when extending nascent transcripts to 30-nt. This susceptibility depends on presence on down-stream DNA, the particular DNA-sequence of the template and presence of transcription factors. Transcription factor TFIIH remains associated to the RNA pol II elongation complex until position +30. At this stage transcription elongation factor TFIIS can rescue stalled transcription elongation complexes. The transcription bubble varies between 13- and 22-nt in size.
|RNA Polymerase II (unphosphorylated):TFIIF complex||DNA-directed RNA polymerase activity (0003899)|
|Human immunodeficiency virus infectious disease||526||[HIV infection]|
|11433015||Analysis of the open region of RNA polymerase II transcription complexes in the early phase of elongation.||Nucleic Acids Res||2001|
|11486021||Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence.||Mol Cell Biol||2001|
|9353262||Promoter escape by RNA polymerase II. Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter.||J Biol Chem||1997|
|7601352||Recycling of the general transcription factors during RNA polymerase II transcription.||Genes Dev||1995|