Formation of the second phosphodiester bond creates a 3-nt product. This transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. At this stage pausing by RNA polymerase II may result in repeated slippage and reextension of the nascent RNA. The transcription complex still requires continued ATP-hydrolysis by TFIIH for efficient promoter escape. Basal transcription factor TFIIE dissociates from the initiation complex before position +10.
Basal transcription factor TFIIF may reassociate and can stimulate transcription elongation at multiple stages. The open region (Ã?Â¢Ã¢â??Â¬Ã?â??transcription bubbleÃ?Â¢Ã¢â??Â¬Ã??) expands concomitant with the site of RNA-extension, eventually reaching an open region from -9 to +9.
|RNA Polymerase II (unphosphorylated):TFIIF complex||DNA-directed RNA polymerase activity (0003899)|
|Human immunodeficiency virus infectious disease||526||[HIV infection]|
|9256425||A role for TFIIH in controlling the activity of early RNA polymerase II elongation complexes.||Proc Natl Acad Sci U S A||1997|
|7601352||Recycling of the general transcription factors during RNA polymerase II transcription.||Genes Dev||1995|
|11739720||Strong natural pausing by RNA polymerase II within 10 bases of transcription start may result in repeated slippage and reextension of the nascent RNA.||Mol Cell Biol||2001|
|9405375||Three transitions in the RNA polymerase II transcription complex during initiation.||EMBO J||1998|