Reactome: A Curated Pathway Database

Addition of nucleotides 5 through 9 on the growing HIV-1 transcript

Stable Identifier
R-HSA-167136
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

Formation of the second phosphodiester bond creates a 3-nt product. This transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. At this stage pausing by RNA polymerase II may result in repeated slippage and reextension of the nascent RNA. The transcription complex still requires continued ATP-hydrolysis by TFIIH for efficient promoter escape. Basal transcription factor TFIIE dissociates from the initiation complex before position +10.

Basal transcription factor TFIIF may reassociate and can stimulate transcription elongation at multiple stages. The open region (?¢â??¬???transcription bubble?¢â??¬??) expands concomitant with the site of RNA-extension, eventually reaching an open region from -9 to +9.

Literature References
Participants
Participant Of
This entity is regulated by:
Title Physical Entity Activity
DNA-directed RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] DNA-directed RNA polymerase activity (0003899)
Inferred From
Disease
Diseases
Name Identifier Synonyms