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RNA polymerase III transcribes microbial dsDNA to dsRNA

Stable Identifier
R-HSA-1964482
Type
Reaction [BlackBoxEvent]
Species
Homo sapiens
Related Species
Human herpesvirus 1, Human gammaherpesvirus 4, Legionella pneumophila
Compartment
Locations in the PathwayBrowser
Summation

RNA polymerase III (POL III) was reported to sense and transcribe cytosolic AT-rich dsDNA into 5'-triphosphate poly(A-U) RNA in human and mouse cells. This dsRNA ligand in turn activated retinoic acid-inducible gene I (RIG-I) leading to production of type I interferon and activation of the transcription factor NF-kappaB (Chiu YH et al. 2009, Ablasser A et al. 2009). Knockdown of POL III expression by siRNA or inhibition of its enzymatic activity by specific chemical inhibitor ML-60218 prevented IFN beta induction in HEK293 cells stimulated with DNA viruses or poly(dA-dT) (Chiu YH et al. 2009, Ablasser A et al. 2009). Moreover, Pol-III inhibition blocked interferon induction by intracellular Legionella pneumophila bacteria [Chiu YH et al 2009].

This project represents cytosolic RNA polymerase III as a complex comprising 17 subunits, although the precise biochemical composition of the cytosolic holoenzyme complex which specifically recognizes AT-rich DNA is not yet known.

Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
RNA polymerase III activity of RNA Polymerase III Holoenzyme [cytosol]
Physical Entity
Activity