Reactome: A Curated Pathway Database

PDPK1 phosphorylates AKT at T308 (R-HSA-198270) [Homo sapiens]


Once AKT is localized at the plasma membrane, it is phosphorylated at two critical residues for its full activation. These residues are a threonine (T308 in AKT1) in the activation loop within the catalytic domain, and a serine (S473 in AKT1), in a hydrophobic motif (HM) within the carboxy terminal, non-catalytic region. PDPK1 (PDK1) is the activation loop kinase; this kinase can also directly phosphorylate p70S6K. The HM kinase, previously termed PDK2, has been identified as the mammalian TOR (Target Of Rapamycin; Sarbassov et al., 2005) but several other kinases are also able to phosphorylate AKT at S473. Phosphorylation of AKT at S473 by TORC2 complex is a prerequisite for PDPK1-mediated phosphorylation of AKT threonine T308 (Scheid et al. 2002, Sarabassov et al. 2005).

Locations in the PathwayBrowser
Additional Information
Compartment cytosol , plasma membrane
Components of this entry
Input entries
Output entries
Catalyst Activity
PhysicalEntity Activity Active Units
p-S-AKT:PDPK1:PIP3 protein serine/threonine kinase activity (0004674)