Reactome: A Curated Pathway Database
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Phosphorylation of TBSMs in LAT

Stable Identifier
R-HSA-202245
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

The adaptor molecule LAT (Linker for the Activation of T cells) is a membrane protein that links the TCR signal to the cell activation. It has a total 10 (Y36, Y45, Y64, Y110, Y156, Y161, Y200, Y220, and Y255) conserved TBSMs (tyrosine based signaling motifs) in its cytoplasmic region. These tyrosine residues are phosphorylated by the activated ZAP-70 upon TCR/CD3 complex engagement. Phosphorylation of LAT creates binding sites for the Src homology 2 (SH2) domains of other proteins, including PLC-gamma1, Grb2 and Gads, and indirectly binds SOS, Vav, SLP-76, and Itk. The residues Y200, Y220 and Y255 are responsible for Grb2 binding, Y200 and Y220 but not Y255, are necessary for Gads binding and Y161 for the PLC-gamma1 binding (numbering based on Uniprot isoform 1).

Literature References
Participants
Participant Of
This entity is regulated by:
Title Physical Entity Activity
protein tyrosine kinase activity of Activated ZAP-70 [plasma membrane] Activated ZAP-70 [plasma membrane] protein tyrosine kinase activity (0004713)
Orthologous Events