MHC II:Ii complexes are internalized in to the endocytic clathrin coated-pit. Dynamin, the GTPase involved in the scission of clathrin-coated vesicles from plasma membrane is observed to be involved in the effective endocytosis of MHC II:Ii complexes. Wang et al, demonstrated that overexpression of a dominant-negative mutant of the GTPase dynamin resulted in the cell surface accumulation of MHC II:Ii complex, supporting that endocytosis is required for delivery to antigen processing compartments (Wang et al, 1997). However, another study using the same dynamin mutant generated opposite conclusions (Davidson, 1999). This discrepancy may be caused by differences in experimental set-up and in the levels of expression of the dynamin mutant and MHC II chains (Dugast et al, 2005).
|Compartment||plasma membrane , cytosol , clathrin-coated endocytic vesicle membrane|
|15749704||AP2 clathrin adaptor complex, but not AP1, controls the access of the major histocompatibility complex (MHC) class II to endosomes||J Biol Chem||2005|
|9202021||Decreased endosomal delivery of major histocompatibility complex class II-invariant chain complexes in dynamin-deficient cells||J Biol Chem||1997|
|10480952||Direct transport of newly synthesized HLA-DR from the trans-Golgi network to major histocompatibility complex class II containing compartments (MIICS) demonstrated using a novel tyrosine-sulfated chimera||J Biol Chem||1999|
|8397411||Cell surface HLA-DR-invariant chain complexes are targeted to endosomes by rapid internalization||Proc Natl Acad Sci U S A||1993|