PARP1 ADP-ribosylates SMAD3 and SMAD4

Stable Identifier
R-HSA-2187325
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

PARP1 ADP-ribosylates SMAD3 and SMAD4 in SMAD2/3:SMAD4 heterotrimer. ADP-ribosyl group is attached to glutamic acid residues E50 and E52 of SMAD3 and unknown amino acid residues of SMAD4. ADP-ribose monomer attached to SMAD3 and SMAD4 is subsequently extended to poly (ADP-ribosyl) chains (i.e. PAR chains) by PARP1, which is not shown here. ADP-ribosylation (PARylation) of SMAD3 and SMAD4 by PARP1 inhibits binding of SMAD2/3:SMAD4 heterotrimers to SMAD binding elements (SBEs) in promoters of SMAD-target genes.

Literature References
PubMed ID Title Journal Year
21095583 PARP-1 attenuates Smad-mediated transcription Mol Cell 2010
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
NAD+ ADP-ribosyltransferase activity of p-2S-SMAD2/3:SMAD4:PARP1 [nucleoplasm]
Physical Entity
Activity
Orthologous Events