Reactome: A Curated Pathway Database
Attention
The EBI data centre will be shutting down from the afternoon (BST) of Friday 26th August until the afternoon of Tuesday 30th August 2016 for essential maintenance. This might have an impact on some Reactome services and we apologize for any inconvenience.

PARP1 ADP-ribosylates SMAD3 and SMAD4

Stable Identifier
R-HSA-2187325
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

PARP1 ADP-ribosylates SMAD3 and SMAD4 in SMAD2/3:SMAD4 heterotrimer. ADP-ribosyl group is attached to glutamic acid residues E50 and E52 of SMAD3 and unknown amino acid residues of SMAD4. ADP-ribose monomer attached to SMAD3 and SMAD4 is subsequently extended to poly (ADP-ribosyl) chains (i.e. PAR chains) by PARP1, which is not shown here. ADP-ribosylation (PARylation) of SMAD3 and SMAD4 by PARP1 inhibits binding of SMAD2/3:SMAD4 heterotrimers to SMAD binding elements (SBEs) in promoters of SMAD-target genes.

Literature References
PubMed ID Title Journal Year
Participants
Participant Of
This entity is regulated by:
Title Physical Entity Activity
NAD+ ADP-ribosyltransferase activity of p-2S-SMAD2/3:SMAD4:PARP1 [nucleoplasm] p-2S-SMAD2/3:SMAD4:PARP1 [nucleoplasm] NAD+ ADP-ribosyltransferase activity (0003950)
Orthologous Events