Reactome: A Curated Pathway Database

NHLRC1 mediated ubiquitination of EPM2A and PPP1RC3 associated with glycogen-GYG1 (R-HSA-3781009) [Homo sapiens]

Black Box Event

NHLRC1 (malin) associates with the glycogen particle where it functions as a ubiquitin E3 ligase to mediate the polyubiquitination of EPM2A (laforin) and PPP1R3C (PTG). The two polyubiquitinated proteins are targeted for proteasome-mediated degradation, leaving a glycogen-GYG1 particle associated with GYS1 (Gentry et al. 2005, Worby et al. 2008). In NHLRC1 knockout mice, PPP1R3C levels are unchanged, rather than increased, suggesting that NHLRC1 does not target PPP1R3C for degradation. However, EPM2A protein levels are increased in this knockout consistent with NHLRC1's proposed role (DePaoli-Roach et al. 2010).

Additional Information
Compartment cytosol
Catalyst Activity
PhysicalEntity Activity Active Units
NHLRC1 ubiquitin-protein transferase activity (0004842)