CERT (ceramide transfer protein), an isoform of COL4A3BP, mediates the translocation of ceramides from the endoplasmic reticulum (ER) membrane to the membrane of the Golgi apparatus. Immunoprecipitation experiments suggest that CERT is associated with the ER membrane as part of a complex with PPM1L (protein phosphatase 1-like) (Saito et al. 2008) and VAPA or VAPB (VAMP-associated proteins A or B) (Kawano et al. 2006). The carboxyterminal START domain of CERT protein specifically binds ceramides (Hanada et al. 2003; Kudo et al. 2008).
|Compartment||endoplasmic reticulum membrane|
|18165232||Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes||J Biol Chem||2008|
|14685229||Molecular machinery for non-vesicular trafficking of ceramide||Nature||2003|
|16895911||Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT||J Biol Chem||2006|
|18184806||Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide||Proc Natl Acad Sci U S A||2008|