Eukaryotic centromeres are marked by a unique form of histone H3, designated CENPA in humans. In human cells newly synthesized CENPA is deposited in nucleosomes at the centromere during late telophase/early G1 phase of the cell cycle. Once deposited, nucleosomes containing CENPA remain stably associated with the centromere and are partitioned equally to daughter centromeres during S phase. A current model proposes that pre-existing CENPA at the centromere drives recruitment of new CENPA, however this has not been proved.
The deposition process requires at least 3 complexes: the Mis18 complex, HJURP complex, and the RSF complex. HJURP binds newly synthesized CENPA-H4 tetramers before deposition and brings them to the centromere for deposition in new CENPA-containing nucleosomes. The exact mechanism of deposition remains unknown.
|GO Biological Process||CENP-A containing nucleosome assembly (0034080)|
|17339380||Propagation of centromeric chromatin requires exit from mitosis||J Cell Biol||2007|
|19410544||Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP||Cell||2009|
|19410545||HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres||Cell||2009|
|20080577||HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres||Proc Natl Acad Sci U S A||2010|
|19410539||Frodos found: Behold the CENP-a "Ring" bearers||Cell||2009|
|19590885||At the right place at the right time: novel CENP-A binding proteins shed light on centromere assembly||Chromosoma||2009|
|19660450||Epigenetic specification of centromeres by CENP-A||Exp Cell Res||2009|
|19629040||Focus on the centre: the role of chromatin on the regulation of centromere identity and function||EMBO J||2009|