Reactome: A Curated Pathway Database

DNA replication initiation (R-HSA-68952) [Homo sapiens]


DNA polymerases are not capable of de novo DNA synthesis and require synthesis of a primer, usually by a DNA-dependent RNA polymerase (primase) to begin DNA synthesis. In eukaryotic cells, the primer is synthesized by DNA polymerase alpha:primase. First, the DNA primase portion of this complex synthesizes approximately 6-10 nucleotides of RNA primer and then the DNA polymerase portion synthesizes an additional 20 nucleotides of DNA (Frick & Richardson 2002; Wang et al 1984).

Additional Information
Compartment nucleoplasm
GO Biological Process DNA replication initiation (0006270)
Literature References
pubMedId Title Journal Year
6693436 DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha. J Biol Chem 1984
11395402 DNA primases. Annu Rev Biochem 2002