Reactome: A Curated Pathway Database

DNA replication initiation (R-HSA-68952)

Species Homo sapiens


DNA polymerases are not capable of de novo DNA synthesis and require synthesis of a primer, usually by a DNA-dependent RNA polymerase (primase) to begin DNA synthesis. In eukaryotic cells, the primer is synthesized by DNA polymerase alpha:primase. First, the DNA primase portion of this complex synthesizes approximately 6-10 nucleotides of RNA primer and then the DNA polymerase portion synthesizes an additional 20 nucleotides of DNA (Frick & Richardson 2002; Wang et al 1984).

Locations in the PathwayBrowser
Additional Information
Compartment nucleoplasm
GO Biological Process DNA replication initiation (0006270)
Literature References
pubMedId Title Journal Year
6693436 DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha. J Biol Chem 1984
11395402 DNA primases. Annu Rev Biochem 2002