Reactome: A Curated Pathway Database

Glycolysis (R-HSA-70171)

Species Homo sapiens


The reactions of glycolysis (e.g., van Wijk and van Solinge 2005) convert glucose 6-phosphate to pyruvate. The entire process is cytosolic. Glucose 6-phosphate is reversibly isomerized to form fructose 6-phosphate. Phosphofructokinase 1 catalyzes the physiologically irreversible phosphorylation of fructose 6-phosphate to form fructose 1,6-bisphosphate. In six reversible reactions, fructose 1,6-bisphosphate is converted to two molecules of phosphoenolpyruvate and two molecules of NAD+ are reduced to NADH + H+. Each molecule of phosphoenolpyruvate reacts with ADP to form ATP and pyruvate in a physiologically irreversible reaction. Under aerobic conditions the NADH +H+ can be reoxidized to NAD+ via electron transport to yield additional ATP, while under anaerobic conditions or in cells lacking mitochondria NAD+ can be regenerated via the reduction of pyruvate to lactate.

Locations in the PathwayBrowser
Additional Information
Compartment cytosol
GO Biological Process canonical glycolysis (0061621)
Cross References
Database Identifier
BioModels Database BIOMD0000000070
Literature References
pubMedId Title Journal Year
16051738 The energy-less red blood cell is lost: erythrocyte enzyme abnormalities of glycolysis Blood 2005