Reactome: A Curated Pathway Database

(S)-Lactate + NAD+ <=> Pyruvate + NADH + H+ (R-HSA-70510)

Species Homo sapiens


Cytosolic lactate dehydrogenase catalyzes the freely reversible reaction of lactate and NAD+ to form pyruvate and NADH + H+. In liver parenchymal cells, this reaction allows lactate from red blood cells and exercising muscle to be converted to pyruvate which in turn is typically used for gluconeogenesis which also consumes the NADH from the reaction.

Lactate dehydrogenase is active as a tetramer. Two isoforms of lactate dehydrogenase, A and B, are widely expressed in human tissues, and all five tetramers - A4, A3B, A2B2, AB3, and B4 - are found. A third isoform, C, and its tetramer, C4, are found in testis.

Locations in the PathwayBrowser
Additional Information
Compartment cytosol
Reverse Reaction Pyruvate + NADH + H+ <=> (S)-Lactate + NAD+
Components of this entry
Input entries
Output entries
Catalyst Activity
PhysicalEntity Activity Active Units
lactate dehydrogenase complex L-lactate dehydrogenase activity (0004459)
Cross References
Database Identifier
Rhea 23447, 23445, 23446