Cytosolic lactate dehydrogenase catalyzes the reversible reaction of pyruvate and NADH + H+ to form lactate and NAD+. In the body under anaerobic conditions such as are found in red blood cells or rapidly exercising skeletal muscle, this reaction regenerates NAD+, allowing the continuation of glycolysis.
Lactate dehydrogenase is active as a tetramer. Two isoforms of lactate dehydrogenase, A and B, are widely expressed in human tissues, and all five tetramers - A4, A3B, A2B2, AB3, and B4 - are found. A third isoform, C, and its tetramer, C4, are found in testis.
|Reverse Reaction||(S)-Lactate + NAD+ <=> Pyruvate + NADH + H+|
|lactate dehydrogenase complex||L-lactate dehydrogenase activity (0004459)|
|11377399||Selective active site inhibitors of human lactate dehydrogenases A4, B4, and C4||Biochem Pharmacol||2001|
|11276087||Structural basis for altered activity of M- and H-isozyme forms of human lactate dehydrogenase||Proteins||2001|
|3435492||The cDNA and protein sequences of human lactate dehydrogenase B||Biochem J||1987|