Cytosolic phosphoglycerate kinase (PGK) catalyzes the reaction of ADP and 1,3-bisphosphoglycerate (1,3BPG) to form D glyceraldehyde 3-phosphate (3PG) and ATP. The active form of the enzyme is a monomer and requires Mg++ (Yoshida and Watanabe 1972; Huang et al. 1980a,b). This is the first substrate level phosphorylation reaction in glycolysis. Two PGK isoforms are known: PGK1 is widely expressed in the body while PGK2 (Chen et al. 1976; McCarrey & Thomas 1987) appears to be confined to sperm cells.
|Reverse Reaction||ATP + 3-Phospho-D-glycerate <=> ADP + 1,3-bisphospho-D-glycerate|
|PGK complexes||phosphoglycerate kinase activity (0004618)|
|1278465||Characterization of phosphoglycerate kinase from human spermatozoa||Fertil. Steril.||1976|
|6771269||Complete amino acid sequence of human phosphoglycerate kinase. Isolation and amino acid sequence of tryptic peptides.||J Biol Chem||1980|
|7391027||Complete amino acid sequence of human phosphoglycerate kinase. Cyanogen bromide peptides and complete amino acid sequence.||J Biol Chem||1980|
|3453121||Human testis-specific PGK gene lacks introns and possesses characteristics of a processed gene||Nature||1987|
|5009693||Human phosphoglycerate kinase. I. Crystallization and characterization of normal enzyme||J Biol Chem||1972|