Reactome: A Curated Pathway Database

Phosphorylation of histone H2AX at Serine-139 by ATM at the site of DSB (R-HSA-75242)

Species Homo sapiens


H2AX phosphorylation (producing the gamma-H2AX protein form) occurs within 1-3 minutes of DNA damage (Rogakou et al,1998) and is promoted by MDC1/NFBD1 (Stewart et al., 2003). gamma H2AX is one of the first proteins to appear at the site of damage, localizing to a region of about 2 Mbp surrounding the site of the double-strand break (Rogakou et al,1998). gamma-H2AX appears to play an essential role in recruiting other repair proteins including Rad50, Rad51 and BRCA1(Paull et al., 2000) (Stewart et al., 2003).

Locations in the PathwayBrowser
Additional Information
Compartment nucleoplasm
Components of this entry
Input entries
Output entries
Catalyst Activity
PhysicalEntity Activity Active Units
p-S1981-ATM protein serine/threonine kinase activity (0004674)
Cross References
Database Identifier
IntAct IM-11070-1
Literature References
pubMedId Title Journal Year
10959836 A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol 2000