Reactome: A Curated Pathway Database

Addition of the third nucleotide on the nascent transcript

Stable Identifier
Reaction [BlackBoxEvent]
Homo sapiens
Locations in the PathwayBrowser

Formation of the second phosphodiester bond creates a 3-nt product. This short transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. The transcription complex still requires continued ATP-hydrolysis by TFIIH and remains sensitive to single-stranded oligo-nucleotide inhibition.

The open region (?transcription bubble?) expands concomitant with the site of RNA-extension. In this case this region spans positions -9 to +3.

Literature References
Participant Of
This entity is regulated by:
Title Physical Entity Activity
helicase activity of TFIIH [nucleoplasm] TFIIH [nucleoplasm] helicase activity (0004386)
DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] DNA-directed 5'-3' RNA polymerase activity (0003899)
Orthologous Events