Reactome: A Curated Pathway Database
Attention
The EBI data centre will be shutting down from the afternoon (BST) of Friday 26th August until the afternoon of Tuesday 30th August 2016 for essential maintenance. This might have an impact on some Reactome services and we apologize for any inconvenience.

Addition of Nucleotides 5 through 9 on the growing Transcript

Stable Identifier
R-HSA-75873
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

Formation of the second phosphodiester bond creates a 3-nt product. This transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. At this stage pausing by RNA polymerase II may result in repeated slippage and reextension of the nascent RNA. The transcription complex still requires continued ATP-hydrolysis by TFIIH for efficient promoter escape. Basal transcription factor TFIIE dissociates from the initiation complex before position +10.

Basal transcription factor TFIIF may reassociate and can stimulate transcription elongation at multiple stages. The open region (?¢â??¬???transcription bubble?¢â??¬??) expands concomitant with the site of RNA-extension, eventually reaching an open region from -9 to +9.

Literature References
Participants
Participant Of
This entity is regulated by:
Title Physical Entity Activity
DNA-directed RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] DNA-directed RNA polymerase activity (0003899)
Orthologous Events