Reactome: A Curated Pathway Database

TRIM33 monoubiquitinates SMAD4 (R-HSA-870449)

Species Homo sapiens


TRIM33 (also known as Ecto, Ectodermin or Tif1-gamma) monoubiquitinates nuclear SMAD4 on lysine residue K519. This leads to disruption of heterotrimeric complexes composed of SMAD4 and two phosphorylated R-SMADs (SMAD2 and/or SMAD3). TRIM33 inhibits SMAD activity without affecting steady state levels of SMAD4 (Dupont et al. 2009, Dupont et al. 2005).

Locations in the PathwayBrowser
Additional Information
Compartment nucleoplasm
Catalyst Activity
PhysicalEntity Activity Active Units
p-SMAD2/3:SMAD4:TRIM33 ubiquitin-protein transferase activity (0004842)