5'-phosphoribosyl-5-aminoimidazole-4-carboxylate (CAIR) + L-aspartate + ATP => 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide (SAICAR) + adenosine 5'-diphosphate + orthophosphate

Stable Identifier
R-GGA-419223
Type
Reaction
Species
Gallus gallus
Compartment
Locations in the PathwayBrowser
Summation

The phosphoribosylaminoimidazolesuccinocarboxamide synthase activity of cytosolic PAICS (multifunctional protein ADE2) catalyzes the reaction of 5'-phosphoribosyl-5-aminoimidazole-4-carboxylate (CAIR), L-aspartate, and ATP to form 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide (SAICAR), ADP, and orthophosphate. Chicken PAICS protein has been purified and its catalytic properties analyzed in vitro (Firestine and Davisson 1994). The native chicken protein behaves as a large multimer on gel filtration; it is inferred to be a octamer by analogy to the human protein (Li et al. 2007). The subcellular location of the chicken enzyme is inferred from that of its better-characterized mammalian counterparts.

Literature References
PubMed ID Title Journal Year
17224163 Octameric structure of the human bifunctional enzyme PAICS in purine biosynthesis

Li, SX, Tong, YP, Xie, XC, Wang, QH, Zhou, HN, Han, Y, Zhang, ZY, Gao, W, Li, SG, Zhang, XC, Bi, RC

J Mol Biol 2007
7918410 Carboxylases in de novo purine biosynthesis. Characterization of the Gallus gallus bifunctional enzyme

Firestine, SM, Davisson, VJ

Biochemistry 1994
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
phosphoribosylaminoimidazolesuccinocarboxamide synthase activity of PAICS octamer [cytosol]
Physical Entity
Activity
Authored