Binding of TFIIA and TFIIB to the pol II promoter:TFIID complex

Stable Identifier
R-HSA-109637
Type
Reaction
Species
Homo sapiens
Compartment
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The general transcription factor TFIIB is a single polypeptide of approximately 35 kDa. There is a Zn-binding domain near the N terminus of TFIIB, and the C-terminal domain encompasses two imperfect repeats; between the N and C termini is a phylogenetically conserved region. The C terminus interacts with TBP and RNA Polymerase II, whereas the N terminus interacts with factor TFIIF and RNA polymerase II. TFIIB is a sequence-specific factor, and it interacts with the BRE element within the promoter.

TFIIB interacts with the Rpb1 subunit of RNA polymerase II to define transcription strat sites. Several activators directly bind TFIIB, and stimulate transcription. The N-terminus and the C-terminus can participate in intramolecular interactions, and this can be disrupted by specific activators by causing a conformational change in TFIIB.

TFIIA also binds the preinitiation complex along with TFIIB. However, TFIIA is not required for accurate initiation, but rather functions as a coactivator of transcription.

Literature References
PubMed ID Title Journal Year
8946909 The general transcription factors of RNA polymerase II.

Orphanides, G, Lagrange, T, Reinberg, D

Genes Dev 1997
Participants
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Orthologous Events
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Created