Reactome | Cleavage of thymine by MBD4 glycosylase

Cleavage of thymine by MBD4 glycosylase

Stable Identifier

R-HSA-110232

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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MBD4 (MED1; methyl-CpG-binding domain protein 4) cleaves thymine mispaired with guanine at CpG islands, which is a consequence of the oxidative deamination of 5-methylcytosine (Petronzelli et al. 2000). The MBD4 catalytic site is located at the C-terminus (Wu et al. 2003). MBD4 may be involved in the maintenance of genomic stability and active DNA demethylation.

Literature References

PubMed ID	Title	Journal	Year
12456671	Mismatch repair in methylated DNA. Structure and activity of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4 Wu, P, Sohail, A, Bhagwat, AS, Zhang, X, Cheng, X, Qiu, C	J. Biol. Chem.	2003
10930409	Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase Yeung, AT, Markham, GD, Stoerker, J, Matsumoto, Y, Bellacosa, A, Riccio, A, Genuardi, M, Seeholzer, SH, Petronzelli, F	J. Biol. Chem.	2000