REV1 inserts dCMP opposite to AP sites in DNA

Stable Identifier
R-HSA-110308
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

REV1 acts as a deoxycytidyl transferase to incorporate a single dCMP opposite a damaged DNA residue. REV1 most efficiently incorporates dCMP opposite apurinic/apyrimidinic (AP, abasic) sites. REV1 enables DNA damage bypass without repair of damaged DNA bases, but its low fidelity results in a mutagenic effect (Nelson et al. 1996, Lin et al. 1999, Gibbs et al. 2000, Zhang et al. 2002).

Literature References
PubMed ID Title Journal Year
11917024 Response of human REV1 to different DNA damage: preferential dCMP insertion opposite the lesion

Zhang, Y, Wu, X, Rechkoblit, O, Geacintov, NE, Taylor, JS, Wang, Z

Nucleic Acids Res. 2002
8751446 Deoxycytidyl transferase activity of yeast REV1 protein

Nelson, JR, Lawrence, CW, Hinkle, DC

Nature 1996
10536157 The human REV1 gene codes for a DNA template-dependent dCMP transferase.

Lin, W, Xin, H, Zhang, Y, Wu, X, Yuan, F

Nucleic Acids Res 1999
10760286 The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light.

Gibbs, PE, Wang, XD, Li, Z, McManus, TP, McGregor, WG, Lawrence, CW, Maher, VM

Proc Natl Acad Sci U S A 2000
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
deoxycytidyl transferase activity of REV1:MonoUb:K164-PCNA:RPA:RFC:AP-DNA Template [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed