Elongation by POLH

Stable Identifier
R-HSA-110319
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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After incorporating two dAMPs opposite the thymine-thymine cyclobutane pyrimidine dimer (TT-CPD), DNA polymerase eta (POLH) can continue translesion DNA synthesis (TLS). POLH preferentially incorporates dAMPs and dGMPs, and may introduce one error per every 18-380 nucleotides (dNMPs) added. POLH stalls after incorporation of a mispaired dNMP, which limits POLH- mediated mutagenesis, in addition to the subsequent polymerase switch (Matsuda et al. 2000, Masutani et al. 2000).

Literature References
PubMed ID Title Journal Year
10801132 Low fidelity DNA synthesis by human DNA polymerase-eta

Matsuda, T, Bebenek, K, Masutani, C, Hanaoka, F, Kunkel, TA

Nature 2000
10856253 Mechanisms of accurate translesion synthesis by human DNA polymerase eta.

Masutani, C, Kusumoto, R, Iwai, S, Hanaoka, F

EMBO J 2000
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
DNA-directed DNA polymerase activity of POLH:MonoUb:K164-PCNA:RPA:RFC:(TT-CPD:AA)-DNA Template [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created