APEX1 mediates endonucleolytic cleavage at the 5' side of the AP site

Stable Identifier
R-HSA-110359
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

APEX1 (APE1, HAP1), a DNA apurinic/apyrimidinic (AP) site DNA lyase, cleaves the DNA strand sugar-phosphate backbone 5' to the AP site generated by DNA glycosylases or that forms by spontaneous loss of a base, producing a DNA strand with a 3'-terminal unsaturated sugar and a DNA strand with a terminal abasic 5'-deoxyribosephosphate (5'-dRP) as cleavage products (Wilson et al. 1995, Bennett et al. 1997, Masuda et al. 1998, Parikh et al. 1998).

Literature References
PubMed ID Title Journal Year
9207062 Interaction of human apurinic endonuclease and DNA polymerase beta in the base excision repair pathway.

Bennett, RA, Wilson, DM, Wong, D, Demple, B

Proc Natl Acad Sci U S A 1997
9724657 Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA

Parikh, SS, Mol, CD, Slupphaug, G, Bharati, S, Krokan, HE, Tainer, JA

EMBO J 1998
7608159 Incision activity of human apurinic endonuclease (Ape) at abasic site analogs in DNA

Wilson, DM, Takeshita, M, Grollman, AP, Demple, B

J. Biol. Chem. 1995
9804798 Dynamics of the interaction of human apurinic endonuclease (Ape1) with its substrate and product.

Masuda, Y, Bennett, RA, Demple, B

J Biol Chem 1998
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity of APEX1:AP-dsDNA [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed