Oxidative demethylation of 1-meA damaged DNA by ALKBH2

Stable Identifier
R-HSA-112118
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
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Oxidative demethylation of 1-meA damaged DNA by ALKBH2
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ALKBH2 catalyzes the removal of the methyl group from 1-methyladenine (1-meA) in a reaction that depends on oxygen, alpha-ketoglutarate and Fe2+. ALKBH2 thus directly reverses alkylation damage of DNA in the form of 1-meA, releasing formaldehyde. ALKBH2 is ~4-fold more active on dsDNA containing 1-methyladenine than 3-methylcytosine (Duncan et al. 2002).

Literature References
PubMed ID Title Journal Year
12486230 Reversal of DNA alkylation damage by two human dioxygenases.

Duncan, T, Trewick, SC, Koivisto, P, Bates, PA, Lindahl, T, Sedgwick, B

Proc Natl Acad Sci U S A 2002
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hasEvent
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Catalyst Activity
Title
DNA-N1-methyladenine dioxygenase activity of ALKBH2:Fe2+:1-meA-dsDNA [nucleoplasm]
Physical Entity
ALKBH2:Fe2+:1-meA-dsDNA [nucleoplasm]
Activity
DNA-N1-methyladenine dioxygenase activity (0043734)
Authored
Pegg, AE  (2004-02-04)
Reviewed
Joshi-Tope, G  (2004-02-04)
Gillespie, ME  (2015-02-06)
Created
Joshi-Tope, G  (2004-04-09)