Cleavage of Procaspase‑9 to caspase‑9

Stable Identifier
R-HSA-114259
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Procaspase‑9 is processed in an ATP‑dependent manner following association with APAF1 and cytochrome c (CYCS) within the apoptosome complex (Li P et al. 1997). However, caspase‑9 (CASP9) has an unusually active zymogen that does not require proteolytic processing (Stennicke HR et al. 1999). Though dispensable for catalytic activity, CASP9 processing was suggested to serve as a "molecular timer" that can limit the proteolytic activity of this complex through displacement of bound caspase‑9 molecules (Malladi S et al. 2009). In addition, this cleavage exposes a neo‑epitope comprising the NH2‑terminal four amino acids (ATPF) of the small p12 subunit of CASP9 that has been shown to be both necessary and sufficient for binding to the baculovirus IAP repeat 3 (BIR3) domain of XIAP, leading to inhibition of CASP9 activity (Srinivasula SM et al. 2001; Shiozaki EN et al. 2003).

Literature References
PubMed ID Title Journal Year
9390557 Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.

Li, P, Nijhawan, D, Budihardjo, I, Srinivasula, SM, Alnemri, ES, Wang, X

Cell 1997
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
cysteine-type endopeptidase activity of APAF1:CYCS:ATP:CASP9(1-416)dimer [cytosol]
Physical Entity
Activity
Authored
Reviewed
Created