Monoubiquitination of N-myristoyl GAG (P12493) protein

Stable Identifier
R-HSA-1169307
Type
Reaction [omitted]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Cytosolic N-myristoyl Gag polyprotein is conjugated with a single molecule of ubiquitin. Conjugation is typically to one of two lysine residues in the p6 domain of Gag but can be to lysine residues in the MA, CA, NC, and SP2 domains of the protein. The specific host cell E2 and E3 proteins that mediate Gag ubiquitination have not been identified. The same studies that first identified the p6 ubiquitination sites in Gag also called the biological significance of Gag ubiquitination into question by demonstrating that Gag proteins in which the p6 ubiquitination sites had been removed by mutagenesis could still assemble efficiently into infectious viral particles (Ott et al. 1998, 2000). More recent work, however, has identified additional ubiquitination sites throughout the C-terminal region of the Gag polyprotein, and when all of these sites are removed by mutagenesis, both viral assembly involving the mutant Gag polyprotein and infectivity of the resulting viral particles are sharply reduced (Gottwein et al. 2006). Note: Reactions directly involving interactions of human host proteins with foreign ones are highlighted in red.

Literature References
PubMed ID Title Journal Year
16775314 Cumulative mutations of ubiquitin acceptor sites in human immunodeficiency virus type 1 gag cause a late budding defect

Gottwein, E, Jager, S, Habermann, A, Krausslich, HG

J Virol 2006
9525617 Ubiquitin is covalently attached to the p6Gag proteins of human immunodeficiency virus type 1 and simian immunodeficiency virus and to the p12Gag protein of Moloney murine leukemia virus

Ott, DE, Coren, LV, Copeland, TD, Kane, BP, Johnson, DG, Sowder RC, 2nd, Yoshinaka, Y, Oroszlan, S, Arthur, LO, Henderson, LE

J Virol 1998
11112487 Ubiquitination of HIV-1 and MuLV Gag

Ott, DE, Coren, LV, Chertova, EN, Gagliardi, TD, Schubert, U

Virology 2000
Participants
Participant Of
This event is regulated
Negatively by
Authored
Reviewed
Created
Cite Us!