Reactome | Mitochondrial transcription termination

Mitochondrial transcription termination

Stable Identifier

R-HSA-163316

Type

Pathway

Species

Homo sapiens

ReviewStatus

5/5

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Transcription of the heavy (H)-strand of mitochondrial DNA (mtDNA) involves two overlapping transcription units (Montoyaet al.,1982; Montoya et al., 1983). The first unit starts right upstream of the tRNAPhe gene and spans the tRNAPhe, rRNA 12S, rRNA 16S and tRNAVal genes (initiation site IH1). The other starts about 100 bp further downstream (initiation site IH2), at the boundary between tRNAPhe and rRNA12S genes, and produces a single polycistronic RNA that encompasses almost the entire length of the H-strand. The ribosomal transcription unit is transcribed at a much higher rate compared to the other transcription unit and control of its expression is exerted both at the level of initiation and termination (Gelfand and Attardi, 1981; Attardi et al., 1990). A central role in the control of termination has been attributed to the mitochondrial transcription termination factor (mTERF), a 39-kDa protein that binds to a 28-base pair region of mtDNA located within the tRNALeu(UUR) gene, at a position immediately downstream of the rRNA 16S gene (Fernandez-Silva et al.,1997; Kruse et al., 1989).

Literature References

PubMed ID	Title	Journal	Year
2276421	Regulation of mitochondrial gene expression in mammalian cells Attardi, G, King, MP, Kruse, B, Polosa, PL, Murdter, NN, Chomyn, A	Biochem Soc Trans	1990
2752429	Termination of transcription in human mitochondria: identification and purification of a DNA binding protein factor that promotes termination Attardi, G, Narasimhan, N, Kruse, B	Cell	1989
3018722	In vitro transcription of human mitochondrial DNA: accurate termination requires a region of DNA sequence that can function bidirectionally Christianson, TW	Proc Natl Acad Sci U S A	1986