Addition of the third nucleotide on the nascent HIV-1 transcript

Stable Identifier
R-HSA-167121
Type
Reaction [BlackBoxEvent]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
Locations in the PathwayBrowser
Summation

Formation of the second phosphodiester bond creates a 3-nt product. This short transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. The transcription complex still requires continued ATP-hydrolysis by TFIIH and remains sensitive to single-stranded oligo-nucleotide inhibition.

The open region (?transcription bubble?) expands concomitant with the site of RNA-extension. In this case this region spans positions -9 to +3.

Literature References
PubMed ID Title Journal Year
9405375 Three transitions in the RNA polymerase II transcription complex during initiation.

Holstege, FC, Fiedler, U, Timmers, HT

EMBO J 1998
11784853 Translocation after synthesis of a four-nucleotide RNA commits RNA polymerase II to promoter escape.

Kugel, JF, Goodrich, JA

Mol Cell Biol 2002
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]
Physical Entity
Activity
Inferred From
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease 526 HIV infection
Authored